燃香拜拜是國人傳統活動行為，但拜香燃燒時產生的懸浮微粒可能附著PAHs及Nitro-PAHs，影響人體健康，一般寺廟中的工作人員、寺廟附近的居民或家中長期燃香的民眾都會接觸這些污染物質而影響健康。

　　本研究為探討不同種類之拜香燃煙所產生之PAHs與Nitro-PAHs，選用了市面上常見九種不同配方和產地的拜香。由於考慮到國人使用拜香的習慣為一次使用三根，所以，本研究中也一次點燃三根拜香，並利用自行設計的採樣器進行採樣，採樣後之濾紙經過萃取、層析、濃縮後以GC-MS分析其中之16種PAHs以及6種Nitro-PAHs，並以元素分析儀得知拜香本身之碳、氫、氧、氮元素含量，最後以Ames Test之沙門桿菌變異種TA98與TA100進行毒性測試比較各種拜香所可能產生的基因毒性。

　　結果顯示拜香燃煙中並不含有Nitro-PAHs，而每種拜香燃燒後所產生的PAHs其特徵差別不大，主要都是3環以上的PAHs為主；其中又以Pa、FL、Pry、CHR為主要(71.9 % ～ 50.4 %)。進行毒性試驗後，結果不添加S9皆無反應，但是，添加S9為皆呈現正反應，顯示不同拜香燃煙的萃取物皆會對人體產生基因毒性。而其毒性大小，以TA98而言，毒性大小為新山香(台)>新山香(中)>老山香(台)>料香(台)>沉香(台)>沉香(中)>檜香(中)>老山香(中)>料香(中)。TA100而言，毒性大小為新山香(台)>新山香(中)>老山香(台)>沉香(台)>老山香(中)>沉香(中)>料香(台)>料香(中)> 檜香(中)。由上述可以得知同一種香而言台灣產的拜香毒性皆大於中國製。因子分析中可以發現，影響拜香燃煙所可能產生毒性的主要因子為燃燒時間。元素組成雖然對於毒性大小並無明顯的相關性，但是同一類拜香中含碳量越高者其排放燃煙之毒性也會越高。

　In this study the Ames Test, which offers reduced variability among experiments, requires smaller quantities of test chemicals and eliminate interactive systematic effects, was used to assess genotoxicity of the incense smoke.

　Nine different types of commercially available incenses were selected for this study. During the sampling period, three sticks were burned at the same time and the suspended particulates collected by a custom-designed sampler. Each sample filter was solvent extracted, fractionated, concentrated, and then analyzed by a GC/MS for PAHs and Nitro-PAHs. Elemental analyses on selected elements, including C, H, O, and N, were also performed for each sample. The concentrated extract from each sample was then tested with Ames Test for its genotoxicity. And the genotoxicity of PAHs and Nitro-PAHs from different incenses were measured.

　The results show that nitro-PAHs were not detected in any incense sample. And the composing of PAHs is alike, 3- or higher rings are major compounds. Especially, Pa, FL, Pry, CHR had the highest contributions (71.9 % ~ 50.4 %). The results of Ames test show that all incences exhibited mutagenic reaction only after adding the S9 liver homogenate. The relative genotoxicity of different incences is Hsing Shan (tw)> Hsing Shan (cn)> Lao Shan (tw)> Liao (tw)> Chen (tw)> Chen (cn)> Gui (cn)> Lao Shan (cn)> Liao (cn) as TA98, and Hsing Shan (tw)> Hsing Shan (cn)> Lao Shan (tw)> Chen (tw)> Lao Shan (cn)> Chen (cn)> Liao (tw)> Liao (cn)> Gui (cn) as TA100. For incenses of similar raw materials, Taiwanese incense had higher toxicity then the corresponding Chinese incense.
　
　Major factors affecting toxicity among different incenses include carbon content and combustion duration. It is imortant to note that incenses with higher carbon content generate smoke with higher genotoxicity.

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