肌酸酐 (Creatinine) 是肌肉中肌酸的代謝物，為診斷人體腎功能的指標性物質，因此人體血液及尿液中的肌酸酐含量，是相當重要的生理檢測項目。本研究乃是合成功能性單體以製備肌酸酐模版高分子膜披覆電極，以電阻抗式分析感測肌酸酐。
功能性單體之製備是以 2-amino-5-bromo-3-nitropyridine (ABNP) 為反應物，以乙烯基取代溴，合成功能性單體 2-amino-3-nitro-5-vinylpyridine (ANVP)，以 1H-NMR 進行分子結構之鑑定，確認單體的合成無誤。
接著以肌酸酐為模版分子，以 ANVP 為功能性單體，以 EGDMA (ethylene glycol dimethacrylate) 為交聯劑，在 AIBN (2,2′-azobisisobutyronitrile) 起始劑的作用下，於金電極表面進行聚合，如是製備之分子模版高分子膜，在有效萃洗出模印的肌酸酐分子後，該高分子膜即可留下具有肌酸酐專一性結合之基座。由 SEM 微影觀察高分子膜之表面結構，由 FT-IR 分析高分子膜萃洗前後特徵鍵之差異，即可確認是否有肌酸酐模印分子被移除。
此模版高分子膜電極將以交流阻抗式進行肌酸酐濃度之感測，以探討阻抗式感測肌酸酐濃度的可行性，並可得知此膜電極的模印表現。由實驗結果得知，當預聚合溶液體積為 7.0 μL，模版分子：功能性單體：交聯劑之莫耳比為 1: 3: 24 時，膜電極有較佳的模印表現，其感測靈敏度為 0.74 ± 0.03 ((Ω – Ωo)/ Ωo)/ (mg/dL)，模印指數為 2.94 ± 0.18。
分子模版高分子膜電極在肌酸共存時對肌酸酐的辨識效果，亦將於本研究中進行討論，由實驗得知，MIP 膜電極對肌酸酐之選擇率為 6.42 ± 0.62。在血清中對肌酸酐的感測效果亦將進行探討，由實驗得知，在血清的干擾下，MIP 膜電極所得之靈敏度為 0.11 ± 0.01 ((Ω – Ωo)/ Ωo)/ (mg/dL)。
綜合前述，確認以模版材料批覆之電極，搭配電阻抗方式的訊號分析，對於肌酸酐之臨床檢測有其可行性。

Creatinine, the metabolite in muscle from creatine, is an important marker for the diagnosis of kidney function. Therefore, human blood and urine creatinine levels are the very important physiological test items. In this study, the functional monomer, 2-amino-3-nitro-5-vinylpyridine (ANVP) was synthesized to assist the production of the creatinine-imprinted polymer film creatinine sensing by AC impedance. ANVP as the monomer, ethylene glycol dimethacrylate (EGDMA) as the crosslinker, and 2, 2′-azobisisobutyronitrile (AIBN) as the initiator, were mixed together in the presence of creatinine as the template for the molecular imprinted polymeric film polymerization on the surface of Au electrode. After the template extracted, the MIP film have the ability to recognize the creatinine by its specific cavity. The most appropriate synthesized ratio is 1:3:24, and the volume of pre-polymerization solution is 7.0 μL. The sensitivity of MIP film electrode is 0.74 ± 0.03 ((Ω – Ωo)/ Ωo)/ (mg/dL), and the imprinting factor is 2.94 ± 0.18. The recognizable of MIP film electrode in the coexistence of creatinine and creatine solution would be confirmed in this study. The selectivity of MIP film electrode toward creatinine is 6.42 ± 0.62. Whether it has the function toward creatinine sensing in the serum based environment was also confirmed.

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