在一般的生醫材料中，鈦合金是廣泛受歡迎的基材之一，而表面改質方法種類繁多，例如機械研磨法、陽極處理法、化學處理法、生物分子黏附法等。其中，實驗中各種影響因子對表面改質後的結果或植入細胞後的生物特性，有不同程度的影響。因此，本研究運用因子設計分析之實驗方法，主要目的分別為探討經表面研磨處理後，藉由紫外光照射鈦合金表面親疏水性之變化；以及藉由電化學之陽極氧化處理建構氧化鈦奈米管柱與測試人類胎兒骨母細胞增殖之反應模式。
本研究提出二個有關紫外光照射鈦合金表面親疏水性之變化與藉由電化學之陽極氧化處理建構氧化鈦奈米管柱與測試人類胎兒骨母細胞增殖之反應模式的結論：(一)、紫外光照射鈦合金表面親疏水性有顯著動態變化；(二)、氧化鈦奈米管柱孔徑大小會影響人類胎兒骨母細胞之增殖反應。

In general, titanium is widely popular one substrate of biomedical materials. There is a wide variety of surface modification methods on titanium alloy, including Grinding, Anodization, Chemical Treatment, Biomolecular Adhesion etc. The functions of surface modification are changed to improve the functionality of the original materials by these methods, thus increasing the biological characteristics of implanted cells. Drawing on factorial design of experiments, the main purposes of this study are including as bellow: (1) the wettability variation on surface of biomedical Ti6Al4V by UV irradiation after grinding. (2) the proliferation model of human fetal osteoblast (hFOB) cells was determined by methylthiazoletetrazolium assay (MTT assay) on TiO2 nanotube arrays.
There are two study reslts as follows. First, the wettabillty on the surface of biomedical Ti6Al4V has significant implications and dynamic changes by UV irradiation. Second, the pore sizes of titanium dioxide nano-column can affect the hFOB cell proliferation.

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