本研究以自製之無針式注射系統進行(內視鏡之噴注性能)測試與體外豬結腸之組織抬升實驗。無針式注射乃採用兩段式噴注方法，第一階段為高壓穿透噴注過程，此階段為噴注穿透豬結腸組織黏膜層；第二段為組織抬升噴注過程乃將抬升液體注入豬結腸組織次黏膜層以產生組織抬升之效果。所採用的抬升液體包括生理食鹽水、甘油溶液及褐藻膠溶液等，噴注性能測試包括流體在長管與短管噴注器中之流率、流速、噴注功率等資料，以作為進行活體實驗之依據。實驗結果顯示褐藻膠溶液在相同黏度下具有較高之噴注功率，亦即可以較低之注射壓力達成所需要的注射效果與注射量，此為採用高流變性液體進行注射的優點。組織穿透實驗結果顯示當第一階段噴注功率在0.5 W以上均可穿透豬結腸之黏膜層，第二階段組織抬升過程，當噴注功率達0.1 W以上時，均可成功地將抬升液體注入組織次黏膜層。組織抬升實驗結果顯示，褐藻膠溶液、甘油溶液與食鹽水溶液之組織抬升高度消散半衰期分別為60分鐘，30分鐘及18分鐘，顯示褐藻膠溶液及甘油溶液具有較長之組織抬升時間，因為內視鏡切除術所需時間為20~30分鐘，故褐藻膠溶液及甘油溶液均符合組織抬升之需要。結果亦顯示，至於抬升液體注射量多寡對於組織抬升時間之消散半衰期並無很大之影響。此組織抬升技術運用於組織冷凍實驗結果顯示，豬結腸若以褐藻膠溶液進行組織抬升，能有效將噴附於結腸表面之冷凍治療物質(二氧化碳雪花)加以阻隔，使下方正常之組織維持正常之溫度，避免冷凍治療物質之低溫對正常組織造成傷害。

This paper investigates the performance of the needle-free injection system with the endoscope for the tissue lift of the porcine colon. Needle-free injection system is two sections of step. The tissue is first penetrated into the mucosal layer and lifted up due to the second-step injection of the working fluids into the tissue. It is then recovered due to the diffusion of the working medium to the neighboring tissue. The working fluids include normal saline glycerin and alginate solutions. Results show that the alginate solution with higher rheology property has the advantage to supply higher flow rate under the same condition. Results also show that when using normal saline as the injection fluid at the first stage. The best injection parameter is injection is injection power of 0.5 W and above. Injection of power more than 0.5 W is ensured to penetrate into the mucosal layer. The second stage jet injection power of 0.1 W and above. Injection of power more than 0.1 W is ensured to carry out tissue elevation. The descending time of alginate and glycerin solution with viscosity of 5cp reduced by 50 % (define as the half-life time of the tissue elevation) is exceeding 60 and 30 minutes. Normal saline has the shortest half-life time of about 18 minutes. The porcine colon used alginate solution to tissue elevation can separate effectively the carbon dioxide snow. The normal tissue part is not harmed by the low temperature of the carbon dioxide snow.

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