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研究生： 黎氏賢
Hien, Le, Thi
論文名稱： 同軸電紡幾丁聚醣-海藻酸鹽芯鞘型奈米纖維及其表面固定銀奈米粒子之抗菌應用
Coaxial Electrospinning of Chitosan/Alginate Core-Sheath Nanofibers with Surface-Immobilized Silver Nanoparticles for Antibacterial Applications
指導教授： 郭昌恕
Kuo, Chang-Shu
學位類別： 碩士
Master
系所名稱： 工學院 - 材料科學及工程學系
Department of Materials Science and Engineering
論文出版年： 2016
畢業學年度： 104
語文別： 英文
論文頁數： 74
中文關鍵詞： 同軸靜電紡絲 、芯鞘型奈米纖維 、幾丁聚醣 、海藻酸鹽 、電漿處理 、銀奈米顆粒 、抗菌測試
外文關鍵詞： coaxial electrospinning, core-sheath nanofibers, chitosan, sodium alginate, plasma treatment, silver nanoparticles, antibacterial test
相關次數： 點閱：227  下載：0 
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    • 本研究利用同軸電紡絲技術製作海藻酸鹽(SA)包覆幾丁聚醣(CS)之芯鞘型奈米纖維，透過添加微量聚氧化乙烯(PEO)在這兩種生物高分子溶液中，幫助其電紡絲製程中形成奈米纖維。將硝酸銀加入外鞘的海藻酸鹽中，再進行氬電漿處理，可以將銀奈米顆粒還原生成並固定在幾丁聚醣奈米纖維表面。這種帶有銀奈米顆粒的芯鞘型奈米纖維對於大腸桿菌(E. coli)具有良好的抗菌能力。
    將海藻酸鹽添加入外鞘溶液中，可以提高基丁聚醣奈米纖維的抗水性，達到強化的效果；因為基丁聚醣表面的胺基會與海藻酸鹽上的羧基形成離子交聯，覆蓋並保護基丁聚醣核心。將基丁聚醣奈米纖維浸泡在酸鹼值為4的水溶液中8小時，其結構仍可以維持穩定，並以掃描式電子顯微鏡實證其芯鞘型奈米纖維形貌。透過紫外光-可見光光譜和穿透式電子顯微鏡分析以電漿合成的銀奈米顆粒之特徵。透過其他分析技術，包含傅立葉轉換紅外光譜、X光光電子能譜，可以獲得更多關於芯鞘型奈米纖維的材料資訊。

    In this research work, coaxial electrospinning technique was used to fabricate the chitosan (CS)/sodium alginate (SA) core-sheath nanofibers from the solutions of these biopolymers and the addition of polyethylene oxide (PEO). Chitosan-based nanofibers with surface-immobilized silver nanoparticles were achieved by the preloaded silver nitrate in the SA sheath, followed by the argon plasma synthesis. The core-sheath nanofibers with Ag NPs demonstrated the promising antibacterial properties against Escherichia coli (E.coli) bacteria. The addition of SA into sheath solution reinforced the chitosan nanofibers in term of their water resistance because of the ionic crosslinking between amine groups of chitosan and carboxyl groups of sodium alginate covering and protecting chitosan cores. Chitosan nanofiber structure remained stable even after 8-hr washing in a pH4 aqueous solution. The morphologies of these core-sheath nanofibers were evidenced by Scanning Electron Microscopy (SEM). The plasma-synthesized Ag NPs were also characterized by ultraviolet-visible spectroscopy (UV-vis) and Transmission Electron Microscopy (TEM). Other characterization techniques, including infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), provided other material information to these unique core-sheath nanofibers.

    Abstract I 摘要 II Acknowledgement III Table of contents IV List of illustration VII List of table X Chapter 1 Introduction 12 1.1 Introduction of Nanofibers 12 1.2 Introduction of electrospinning method 13 1.2.1 Single electrospinning 13 1.2.2 Co-axial electrospinning 14 1.3 Chitosan and electrospinning Chitosan 17 1.4 Sodium Alginate and Electrospinning Sodium Alginate 19 1.5 Ag NPs and Antibacterial properties of Ag NPs 20 Chapter 2 Motivation 22 Chapter 3 Experimental Section 24 3.1 General preview 24 3.2 Materials and Experimental Instruments 25 3.3 Fabrication of Coaxial Spinneret 25 3.4 Preparation of the Electrospinning Solution 27 3.4.1 Electrospinning solution used for single electrospinning 27 3.4.2 Electrospinning solution used for coaxial electrospinning 27 3.2.2.1 Core solution 27 3.2.2.2 Sheath solution 28 3.5 Electrospinning 29 3.5.1 Single electrospinning 29 3.5.2 Coaxial electrospinning 29 3.6 Synthesis of Ag Nanoparticles by Plasma Treatment 30 3.7 Nanofibers Characterization 30 3.7.1 Scanning electron microscopy (SEM) 30 3.7.2 Transmission electron microscope (TEM) 31 3.7.3 Ultraviolet-visible spectroscopy (UV-vis) 32 3.7.4 Fourier transform infrared spectroscopy (FT-IR) 32 3.7.5 X-ray photoelectron spectroscopy (XPS) 33 3.7.6 Antibacterial test 33 Chapter 4 Results and Discussion 38 4.1 Material fabrications 38 4.1.1 Chitosan Single Nanofibers and Strength in Water Environment 38 4.1.2 Chitosan Coaxial Nanofibers 40 4.1.2.1 As-spun Chitosan-Sodium Alginate (CS-SA) Coaxial Nanofibers 40 4.1.2.2 As-spun Chitosan-Sodium Alginate-Silver (CS-SA-Ag) Coaxial Nanofibers 42 4.2 Core-Sheath Structure 44 4.2.1 TEM 44 4.2.2 Diameter Comparison Before and After Outer Layer Removal 45 4.2.3 Controlling the Outer Layer Thickness 47 4.3 Ar-Plasma Treatment 50 4.3.1 Diameter reduction and plasma etching rate 50 4.3.2 Ag NPs formation 52 4.3.2.2 TEM image of core-shell nanofibers before and after plasma treatment 54 4.3.4 XPS analysis 55 4.3.3 Surface immobility of Ag NPs on nanofibers 60 4.4 Crosslinking between Chitosan and Sodium Alginate 62 4.4.1 Water resistance test 62 4.4.2 Effects of plasma treatment on crosslinking 64 4.4.3 FTIR analysis 66 4.5 Antibacterial Test 68 Chapter 5 Conclusions 70 Chapter 6 Reference 71

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