桑黃菇是一種傳統中草藥，被發現有顯著的抗腫瘤功能。本著強而有力的療效，桑黃在韓國成了癌症輔助治療的核可藥物。其多醣體被發現有多種免疫調節功能。例如，一項腫瘤小鼠的體內實驗顯示，桑黃分離出的醣蛋白，可以讓助手型T淋巴細胞分化趨向第一型的免疫型式，並且抑制MCA-102癌細胞的生長。不幸地，桑黃子實體在野外生長緩慢。因此，以液態培養出桑黃菌絲體，再濃縮其中有效的分離物，會是較為迅速的研究方法。所以，我們想知道此種液態培養的桑黃，是否能造成如前人所說的腫瘤抑制及免疫調節反應。桑黃樣本被分為菌絲體水煮液（PLw）、水煮液中的多醣體（PLPS）及桑黃發酵過的培養液（PLm）。一開始先以C57BL/6j小鼠做28日藥物毒性測試，最高劑量組給予1000毫克/公斤體重．日之下，可看出桑黃的安全性。在C57BL/6j鼠接種小鼠肺癌細胞LL/2腫瘤模型中，PLm+PLw及PLw有抑制腫瘤效果，但PLPS則否。PLm+PLw在200及400毫克/公斤體重．日的服用下，35天後對腫瘤體積抑制率分別為38﹪及36﹪；PLw在200及400毫克/公斤體重．日的服用下，36天後對腫瘤體積抑制率分別為61﹪及67﹪。然而，體外實驗顯示了PLm及PLw對LL/2及人類肺癌細胞A549的活性抑制效果，以及Sub-G1值的提升。脾臟中細胞的CD4/CD8比值，及血清中IL-12值沒有明顯改變。以PLPS刺激小鼠巨噬細胞RAW 264.7可以提升其吞噬能力。因此，我們的結論認為液態培養的桑黃在體外有抑制癌細胞及免疫調節功能，但是在動物模型的效果則需要進一步釐清。

Phellinus linteus (PL) is a traditional Chinese herbal medicine and found to have remarkably tumor-inhibiting functions. With powerful therapeutic effects, PL becomes an approved medicine in Korea for the co-treatment of cancer. Its polysaccharides (PS) are found to have immune-modulate functions. An in vivo mice tumor model shows that the proteoglycan isolated from PL may lead type-I helper T cell-dominant immune state and inhibit the growth of MCA-102 cells. Unfortunately, the PL fruit body grows slowly on the wild field. Therefore, liquid culture of the PL mycelia and then concentration of the functional fractions is a more rapid method to study on its effects. Thus, we want to know whether the liquid cultured PL causes tumor-inhibiting and immune-modulating reactions as the previous studies show. PL samples were separated into water extractions (PLw), polysaccharides in PLw (PLPS), and PL broth medium (PLm). A 28-days drug toxicity test was performed in the beginning by treating C57BL/6j mouse with the highest dosage of 1000 mg/kg．day and showed the safety of PL. PLm+PLw and PLw have tumor-inhibiting functions to LL/2 tumor bearing C57BL/6j male mice, but PLPS doesn’t. The tumor inhibition rates came to 38﹪ and 36﹪ under 35 days treatment of 200 and 400 mg/kg．day of PLm+PLw； 61﹪ and 67﹪ under 36 days treatment of 200 and 400 mg/kg．day of PLw. However, the in vitro assay shows the inhibitory effects of PLm and PLw to LL/2 and human lung carcinoma A549 and the up-regulation of their Sub-G1 values. The ratios of CD4/CD8 of spleenocytes and the level of IL-12 in serum had no obvious change. Stimulation of RAW 264.7 cells by PLPS may enhance the abilities of its phagocytosis. Therefore, we conclude liquid culture system of PL has tumor-inhibiting and immune-modulating functions in vitro, but the effects to the animal model should be further elucidated.

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