急性肝胰腺壞死症 (Acute hepatopancreatic necrosis disease AHPND)為造成亞洲蝦類水產養殖產業嚴重衝擊的細菌性疾病 導致 AHPND 的致病性細菌為帶有二源毒素 PirAB VP 基因質體 pVA 1 的副溶血弧菌Vibrio parahaemolyticus VP) 。現今對於 AHPND 的致病機轉與宿主反應尚未明確 因此 本實驗利用系統生物學的方法，結合代謝組與轉錄組找出感染 AHPND 後的蝦胃有哪些特定的代謝路徑變化以深入了解 AHPND的致病機轉。 利用 U HPLC QTOF MS based 代謝體學分析，在正離子與負離子模式下 獲取 503 以及 634 種感染 AHPND 後之蝦胃所表現的代謝物，隨後將 所得之代謝物結合實驗室轉錄組資料庫分析感染 AHPND 後的蝦胃代謝路徑藍圖。特定脂質代謝路徑於蝦隻感染 AHPND 後的胃部發生改變 為更深入了解有哪些基因參與在這些路 徑 中，建立候選基因的共表現網絡並以即時聚合酶連鎖反應進行驗證。 從這些結果中發現，cytosolic phospholipase A2 以及 JHE like carboxylesterase 在感染後的蝦胃中基因表 現顯著上升。這些發現有助於更進一步了解 AHPND 的致病機轉並建立對蝦類養殖產業有效的生物安全對策。

Acute hepatopancreatic necrosis disease (AHPND) has caused huge losses in Asia’s shrimp aquaculture industry. Although the etiology is an infective bacteria Vibrio parahaemolyticus (VP) harboring a distinct virulence plasmid (pVA1) containing a binary toxin PirABVP, host responses and molecular pathogenesis are not fully elucidated. Therefore, a systems biology approach was used to pick out critical pathway[s] in the transcriptome and metabolome of the stomach of shrimp infected with AHPND. By using UHPLC-QTOF-MS-based metabolomics profiling, metabolites which differentially expressed were collected from positive and negative ion modes, respectively. Metabolites were integrated with our in-house transcriptome database to assess comprehensive host responses in stomach of shrimp during AHPND infection. Using this strategy, we confirmed that some lipid metabolism pathways were dysregulated in the stomach of AHPND-infected shrimp. Furthermore, a gene-to-gene correlation network was constructed to find candidate gene, with gene expression established using real-time PCR. In those studies, JHE-like carboxylesterase (JHE-L CE) and cytosolic phospholipase A2 (cPLA2) were considerably expressed in the stomach of shrimp infected with AHPND. These findings may help in understand the AHPND pathogenesis and should improve biosecurity and reduce impact of AHPND in shrimp aquaculture.

張哲輊，利用轉錄體學探討蝦感染急性肝胰腺壞死病之宿主反應，國立成功大學生物科技研究所碩士論文，2016。

葉民德，探討蝦類Toll及IMD路徑在急性肝胰腺壞死症致病過程中所扮演的角色，國立成功大學生物科技研究所碩士論文，2015。

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