本研究利用PIC法及Navier-Stokes方程式與絕對遷移率，數值模擬帶電溶質離子在毛細管電泳晶片內之遷移現象，並從傳統十字型微管道的毛細管電泳晶片改變為具擴張式分離管道的外形。再分別由分離端總長在L3/h=250和L3/h=1000兩個情況之下，藉由 (i)擴張段與十字交會處的距離不同 (ii)擴張段的長度不同 (iii)分離管道兩端之管徑不同 (iv)微管道內雷諾數( Reynolds number, Re )的不同，四個方向來對具有擴張式分離管道的毛細管電泳晶片內不同電性之溶質離子遷移現象進行討論。且由以上討論之參數，找出對具擴張式分離管道外形之帶電離子遷移現象較為顯著的影響，並觀察其分離檢測過程時之電荷密度。
由結果顯示，在擴張段與十字交會處的距離不同時，對帶電離子在毛細管電泳微管道內之遷移現象會有較為顯著的影響。故再針對其分離檢測過程作電荷密度之分析。可發現在L3/h=1000時，帶正電離子之電荷密度較高，且其負離子之分離檢測效果也比在L3/h=250時好。並可知帶正負電溶質離子會隨著分離時間的增加而分離的愈明顯。

This study employed the particle-in-cell (PIC) method, Navier-Stokes equation, and absolute mobility to simulate charged solute ionic migration in capillary zone electrophoresis (CZE) chip, especially in an expansive separation-channel CZE system. Under the condition of L3/h=250 and L3/h=1000, the present study describes the migration of differently charged solute ions in an expansive separation-channel CZE system in terms of (i) different distance between expansion section and intersection, (ii) different length of expansion section, (iii) different width of both-end of separation channel, and (iv) different Reynolds number (Re) in microchannel. Furthermore, based on the four parameters above-mentioned, this study is to find out the significant effect on the migration of charged ions in an expansive separation-channel CZE system, and to observe the charge density in the detection process.
The results reveal that when the distance of expansion section and intersection differs, the expansive channel shows a significant effect on the migration of charged ions in CZE system. Besides, according to the analysis of charge density in the separation detect process, it shows that the charge density of positive ions is higher, and the detection effect of negative ions are better when L3/h=1000. Furthermore, the positive and negative charged ions are separation apparent that when separation detect time increase.

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