本研究主要在實驗室半連續泥漿環境中，了解現地族群菌種微生物是否能被馴養以甲苯為基質共代謝生物降解三氯乙烯(TCE)；另觀察在同樣條件下植入甲苯分解菌T1菌時，對於三氯乙烯的生物降解共代謝行為，以作為當現地菌無法成功馴養的另一選擇。
半連續馴養操作簡單，可迅速評估生物復育之可行性，適用在土壤地下水污染場址整治方法之初期篩選。
就菌數而言，多落在10^5~10^6 cells/ml 之間；營養鹽純氧曝氣至32ppm以上，溶氧消耗(包括實驗過程非生物性損耗)多在25~30ppm，因此溶氧充足；根據單一培養時程實驗結果，幾乎沒有中間產物的產生，僅發現在約10小時前後產生Vinyl Chloride等中間產物且濃度很低，顯示本實驗處於穩定的好氧環境。根據現地組II與現地組I的三氯乙烯移除率比較，趨勢結果相似，亦顯示操作過程對實驗結果的變異性很小，操作穩定。
在三氯乙烯濃度約640ppb, 甲苯濃度約9500ppb 的情況下，植種組三氯乙烯共代謝去除率達65.4+-6.8 %，兩次現地組各達67.5+-9.9 %及73.5+-12.2 %。穩態下三氯乙烯共代謝去除率達76.5+-7.4 %。
建議未來進一步執行連續流試驗研究；再經現地模廠試驗驗證現地之生物共代謝降解效率；最後實際應用在台灣本土三氯乙烯之污染場址。

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