石斑魚為世界高經濟價值之養殖魚種，而點帶石斑魚 (Epinephelus coioides) 在臺灣更是經濟主要魚種之一。然而因爲飼養方式過高密度，易引起疫病的暴發，尤其像野田病毒 (nodavirus) 的侵害，造成大量幼魚死亡。先前本實驗室的研究顯示，病毒感染后石斑魚體内會誘導第一型干擾素 (interferon, IFN) 相關基因大量表現。干擾素為分泌型細胞激素，受到誘導後表現，會分泌至細胞外 ，結合鄰近細胞上的干擾素受器 (interferon receptor, IFNR)，活化傳遞功能引導下游免疫基因啓動機制。過去哺乳類研究報導在病毒感染下干擾素與其接受器結合后，活化下游免疫基因轉錄動作，如 Mx。因此，在本研究中想籍由觀察點帶石斑魚干擾素與其接受器結合後續的反應與其免疫角色，首先選殖出點帶石斑魚第一型干擾素接受器 1 (orange-spotted grouper type I IFNR1) 之序列，並以感染野田病毒的點帶石斑魚作爲試驗材料，觀察在病毒感染的不同時間點下 IFNR1 的表現量，最後建立表現與純化 IFNR1-EX 重組蛋白平臺。研究結果將對於往後點帶石斑魚在野田病毒防治上具參考價值。

Grouper has been known as a high commercial value fish species in the world, in Taiwan, orange-spotted grouper is known as one of the major economy income population. However, due to improper cultivation method, disease like nodavirus infection breakout easily among the species. Previous studies indicated induction of type I interferon and related genes in infected grouper species, interferon is known as secreted protein that can be activated upon challenge, and released to neighboring cells, interferon receptor on neighboring cell would interact with interferon and transduce signal to downstream activity for activate antiviral mechanism. In mammalian studies, when host undergo infection, it would activate downstream antiviral activity such as Mx production. Thus in this study, through studying interferon system of orange-spotted grouper that been proved as an antiviral cytokine in fight against viral infection, what is the interaction of cytokine to cytokine receptor and relationship correspond to immune response. Firstly, by cloning of full length sequence of osgIFNR1, analyze mRNA expression level of osgIFNR1 with different immune stimulants and viral infection. Overexpression of IFNR1 is carried out to observe localization in cell line to provide evidences indicating response of IFNR1 in normal and infected condition. Further, constructed plasmid expressing recombinant protein of IFNR1 in orange-spotted grouper is established for in-depth functional analysis. All of these results would contribute to the prevention of nodavirus infection in orange-spotted grouper for future days.

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