顳葉癲癇為一種常見的慢性腦部神經疾病，病患中有將近三成無法由藥物有效控制，其中適用切除手術的病患又佔少數，因此有必要發展新的治療方法以抑制癲癇之發作。本研究目的為利用光基因刺激發展即時閉迴路控制系統以抑制癲癇發作。首先，在ChR2光基因轉殖鼠上施打鋰鹽與毛果芸香鹼以誘導急性與慢性癲癇，應用此動物模型模擬人類顳葉癲癇，由立體定位手術在小鼠海馬迴腦部雙側植入紀錄電極及光纖，並使用樣本熵法來做為辨識癲癇的指標。接著，以擷取的深腦電訊號作為回饋訊號設計光刺激的控制器，再將辨識指標與控制器結合，以樣本熵法辨識癲癇狀態，並以分段線性比例的控制法則調整光強度，建構出閉迴路光刺激控制系統。由10隻小鼠實驗結果顯示本研究發展的控制系統在急性癲癇發作期間辨識率高達95%以上，而在慢性期辨識率約在85%~90%之間，樣本熵可有效的分辨出正常狀態與癲癇發作，而在抑制成效上急性與慢性期間光刺激皆能快速且有效的抑制癲癇發作，兩者抑制成功率皆高於80%，在慢性期間最高可達98.3%。總之，樣本熵與光刺激技術可對未來顳葉癲癇治療提供神經刺激裝置新的發展方向。

Temporal lobe epilepsy is the common form of partial epilepsy. Although antiepileptic drugs have been found, there are still 30% of patients who cannot benefit from these treatments. Only a minority of patients are suitable for resection of lesion sites, so new method needs to be developed to inhibit seizure. The purpose of this study is to develop a control system to suppress chronic seizure by using photic stimulation. To simulate human seizure in the animal experiment, the Thy1-ChR2-YPF transgenic mice were injected with lithium and pilocarpine to induce acute and chronic seizure. Then, two electrodes and an optical fiber were implanted into the hippocampus of the mice. Depth-EEG measured from mice is used to design the detection and control system. The sample entropy calculated from contralateral depth-EEG was utilized to recognize epileptics state and to adjust the intensity of photic stimulation by using a piecewise linear proportional law in the control system. The results from 10 subjects showed seizure recognition rate can rise up to 95% in the acute stage and 85 to 90% in the chronic stage. The photic stimulation can swiftly and effectively inhibit seizure for both acute and chronic stages, and the highest suppression rate achieved was 98.3% in the chronic stage. In conclusion, sample entropy method and photic stimulation technology may provide a new direction of development on treating temporal lobe epilepsy in the future.

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