本研究利用微測壓管方法與eDNA分子生物學技術，希望能夠了解伏流水層其上湧、下滲特性對於eDNA運輸的機制的影響，假設在下滲區域因其下滲特性會將eDNA分子帶進伏流水層，所以eDNA濃度相對來說應該會比較高，反之，上湧區域因其上湧特性將水流從伏流水層流入溪流會使顆粒不易沉降，所以eDNA濃度應該會比較低；eDNA技術可用來監測水域生態系統中生物，但是eDNA在運輸過程中會受到許多因素影響，例如：光照、pH值等環境因子、河床底質以及流態變化，所以了解影響流動水中eDNA運輸過程的物理和生物變量至關重要。
釋放實驗的結果佐證了eDNA的特性，其異質性與多分散性，會導致eDNA在水中的動力學像是顆粒的表現，在水中並非呈現均勻分佈的情形，運輸過程中因為其尺寸範圍跨越大，所以不會表現出一致的運輸模式。表面水的eDNA濃度變化大，雖然研究區域的範圍小以及其流量並非太大，但是其地形與底質造成的流態變化強烈影響eDNA在表面水的運輸，所以預先了解流態的變化，應該能增進對於理解eDNA在溪流中的運輸機制。從結果得知伏流水與表面水的eDNA濃度比值約為0 ~ 20 %，若是不考慮再懸浮，那應該可以設定為每個時間段eDNA濃度會被保留的比例；另一方面，從結果得知上湧、下滲區域特性對於進入伏流水層eDNA濃度，與原假設相反，反而在上湧區域的伏流水eDNA濃度皆大於下滲區域的eDNA濃度，但最大的原因可能是其流態的變化影響。

In this study, we tried to understand the influence of the upwelling and downwelling in the hyporheic zone on the transport of eDNA. It was assumed that eDNA molecules would infiltrate into the hyporheic zone due to downwelling, so eDNA concentration in the hyporheic zone should be relatively high. On the contrary, the water in upwelling zone would bring up water from the hyporheic zone into the stream, therefore, eDNA particles would not be easily deposited on the riverbed and the concentration of eDNA should be relatively low compared to the downwelling site. The results showed that the characteristics of eDNA concentration in the upwelling and downwelling regions were opposite to the original hypothesis that the eDNA concentration would be high in the hyporheic zone. The concentration of eDNA in the upwelling region was greater than in the downwelling region. The concentration of eDNA in the stream was also compared to that in the hyporheic zone. The ratio of eDNA concentration in hyporheic zone to that in the stream was about 0 to 20%. Changes in flow pattern might be caused by the topography, and the substrate strongly influence the transport of eDNA on surface water. Changes in flow pattern should be known in advance to prevent the uncertainity in the understanding of eDNA transport in the stream.

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