藍綠菌有許多生理機制使他們容易成為水中的優勢種，當藻華發生時，藍綠菌大量繁殖同時也會伴隨著二次代謝物的產生，包含藻毒及藻臭，本研究主要針對2-MIB進行實驗。
具有土霉味的2-MIB與土臭味的Geosmin被視為世界各地飲用水源中主要的臭味物質(T&O compounds)，這些氣味對人體並沒有危害，但容易引起民眾對飲用水品質的疑慮。由於不同的藍綠菌有相對應的處理程序，因此若能了解產生毒素及臭味的主要藍綠菌便能增加水源管理效率。
目前對於2-MIB的監測，主要是以氣相層析質譜儀(Gas chromatography- Mass Spectrometry, GC-MS)檢測2-MIB的濃度，另外也會搭配DNA萃取及即時定量聚合酶連鎖反應（qPCR）來分析水中產2-MIB基因的濃度，但以上兩種方式都只能定量其濃度而不能得知組成比例，若是以顯微鏡檢測，雖然可以得知每一種藍綠菌的數量，但是許多藻的外型十分相像，像是顫藻目下不同的屬就無法以肉眼分辨，另外並非每一株藻都具有產毒或產臭的功能基因，因此我們無法以藻數來斷定功能基因的數量。
本研究使用第三代基因定序(Oxford Nanopore Minion)對水體中的2-MIB進行基因定序及分析，研究場址則以台灣本島、金門及馬祖的水庫為主，希望能透過基因定序及基因相似度比對來得到水體中產2-MIB的藍綠菌組成，並提供結果給水處理單位，幫助他們可以快速解決2-MIB的主要生產者。
此外，本研究也建立底泥中2-MIB的萃取方法及檢測方法，並透過GC-MS及分子生物技術（qPCR、ddPCR）了解2-MIB在底泥與水中的濃度分布，並由此了解該區域2-MIB主要產生源是水還是底泥，才能採取相對應的應變措施。

Cyanobacteria possess diverse physiological mechanisms that grant them dominance in aquatic environments. During cyanobacterial blooms, the significant proliferation of cyanobacteria is often accompanied by the synthesis of secondary metabolites, including toxins and odorous compounds. This study is centered around one particular odorous compound produced by cyanobacteria—2-methylisoborneol (2-MIB).
2-MIB, characterized by its musty odor, along with geosmin, which imparts an earthy scent, are renowned as the most frequently detected taste and odor (T&O) compounds in global drinking water sources. Although these T&O compounds are not detrimental to human health, they can elicit concerns about water quality among the public. The identification of primary cyanobacteria responsible for toxin and odor production can significantly enhance water management efficiency, given that distinct cyanobacteria necessitate specific treatment approaches.
This study employs third-generation gene sequencing (Oxford Nanopore Minion) to sequence and analyze genes associated with 2-MIB production within water bodies. By sequencing genes and comparing their similarities, we can ascertain the cyanobacteria composition responsible for 2-MIB production in water. Sharing these findings with water treatment units can aid in swiftly addressing the major contributors to 2-MIB production.
Moreover, this research establishes an extraction method and detection technique for 2-MIB in sediment. We utilize GC-MS and molecular biology techniques (qPCR and ddPCR) to assess the distribution of 2-MIB concentrations in both sediment and water bodies. This enables the determination of whether the principal source of 2-MIB in each area is water or sediment, thereby facilitating the implementation of suitable contingency measures.

Alster, A., Kaplan-Levy, R. N., Sukenik, A., & Zohary, T. (2010). Morphology and phylogeny of a non-toxic invasive Cylindrospermopsis raciborskii from a Mediterranean Lake. Hydrobiologia, 639, 115-128.
Arya, M., Shergill, I. S., Williamson, M., Gommersall, L., Arya, N., & Patel, H. R. (2005). Basic principles of real-time quantitative PCR. Expert review of molecular diagnostics, 5(2), 209-219.
Bahnwart, M., Hübener, T., & Schubert, H. (1998). Downstream changes in phytoplankton composition and biomass in a lowland river–lake system (Warnow River, Germany). Hydrobiologia, 391, 99-111.
Beer. (1852). Bestimmung der Absorption des rothen Lichts in farbigen Flüssigkeiten. Annalen der Physik, 162(5), 78-88.
Bhat, S., Curach, N., Mostyn, T., Bains, G. S., Griffiths, K. R., & Emslie, K. R. (2010). Comparison of methods for accurate quantification of DNA mass concentration with traceability to the international system of units. Analytical chemistry, 82(17), 7185-7192.
Breitbach, S., Tug, S., Helmig, S., Zahn, D., Kubiak, T., Michal, M., . . . Simon, P. (2014). Direct quantification of cell-free, circulating DNA from unpurified plasma. PLoS One, 9(3), e87838.
Butler Sr, B. R. (1999). Examination of the effects of barley straw (Hordeum vulgare) on freshwater algae in the field and laboratory. Hood College,
Callahan, B. J., McMurdie, P. J., & Holmes, S. P. (2017). Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. The ISME journal, 11(12), 2639-2643.
Cao, Y., Sivaganesan, M., Kinzelman, J., Blackwood, A. D., Noble, R. T., Haugland, R. A., . . . Weisberg, S. B. (2013). Effect of platform, reference material, and quantification model on enumeration of Enterococcus by quantitative PCR methods. Water Research, 47(1), 233-241.
Carey, C. C., Ibelings, B. W., Hoffmann, E. P., Hamilton, D. P., & Brookes, J. D. (2012). Eco-physiological adaptations that favour freshwater cyanobacteria in a changing climate. Water Research, 46(5), 1394-1407.
Carpenter-Boggs, L., Loynachan, T., & Stahl, P. (1995). Spore germination of Gigaspora margarita stimulated by volatiles of soil-isolated actinomycetes. Soil biology and biochemistry, 27(11), 1445-1451.
Carter, M. C., Weber Jr, W. J., & Olmstead, K. P. (1992). Effects of background dissolved organic matter on TCE adsorption by GAC. Journal‐American Water Works Association, 84(8), 81-91.
Catherine, Q., Susanna, W., Isidora, E.-S., Mark, H., Aurélie, V., & Jean-François, H. (2013). A review of current knowledge on toxic benthic freshwater cyanobacteria–ecology, toxin production and risk management. Water Research, 47(15), 5464-5479.
CCALA, Culture collection of autotrophic organisms. Retrieved from https://ccala.butbn.cas.cz/en
Chen, G., Dussert, B., & Suffet, I. (1997). Evaluation of granular activated carbons for removal of methylisoborneol to below odor threshold concentration in drinking water. Water Research, 31(5), 1155-1163.
Chiu, Y.-T., Yen, H.-K., & Lin, T.-F. (2016). An alternative method to quantify 2-MIB producing cyanobacteria in drinking water reservoirs: Method development and field applications. Environmental Research, 151, 618-627.
Chu, Z., Jin, X., Iwami, N., & Inamori, Y. (2007). The effect of temperature on growth characteristics and competitions of Microcystis aeruginosa and Oscillatoria mougeotii in a shallow, eutrophic lake simulator system. Hydrobiologia, 581, 217-223.
Cook, D., Newcombe, G., & Sztajnbok, P. (2001). The application of powdered activated carbon for MIB and geosmin removal: predicting PAC doses in four raw waters. Water Research, 35(5), 1325-1333.
Corinaldesi, C., Danovaro, R., & Dell'Anno, A. (2005). Simultaneous recovery of extracellular and intracellular DNA suitable for molecular studies from marine sediments. Applied and environmental microbiology, 71(1), 46-50.
Cyanophyceae: Nostocales: Oscillatoriaceae Retrieved from http://protist.i.hosei.ac.jp/PDB/Images/Prokaryotes/Oscillatoriaceae/Lyngbya/Lyngbya_3.html
Czerny, J., Barcelos e Ramos, J., & Riebesell, U. (2009). Influence of elevated CO 2 concentrations on cell division and nitrogen fixation rates in the bloom-forming cyanobacterium Nodularia spumigena. Biogeosciences, 6(9), 1865-1875.
Deere, D., Porter, J., Pickup, R., & Edwards, C. (1996). Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms. Journal of Applied Bacteriology, 81(3), 309-318.
Devi, A., Chiu, Y.-T., Hsueh, H.-T., & Lin, T.-F. (2021). Quantitative PCR based detection system for cyanobacterial geosmin/2-methylisoborneol (2-MIB) events in drinking water sources: Current status and challenges. Water Research, 188, 116478.
Dong, P., Wang, H., Fang, T., Wang, Y., & Ye, Q. (2019). Assessment of extracellular antibiotic resistance genes (eARGs) in typical environmental samples and the transforming ability of eARG. Environment international, 125, 90-96.
Doucette, W. J. (2003). Quantitative structure‐activity relationships for predicting soil‐sediment sorption coefficients for organic chemicals. Environmental Toxicology and Chemistry: An International Journal, 22(8), 1771-1788.
Dvořák, P., Poulíčková, A., Hašler, P., Belli, M., Casamatta, D. A., & Papini, A. (2015). Species concepts and speciation factors in cyanobacteria, with connection to the problems of diversity and classification. Biodiversity and Conservation, 24, 739-757.
Edgar, R. C. (2018). Updating the 97% identity threshold for 16S ribosomal RNA OTUs. Bioinformatics, 34(14), 2371-2375.
Faluweki, M. K., & Goehring, L. (2022). Structural mechanics of filamentous cyanobacteria. Journal of the Royal Society Interface, 19(192), 20220268.
Fastner, J., Erhard, M., & von Döhren, H. (2001). Determination of oligopeptide diversity within a natural population of Microcystis spp.(Cyanobacteria) by typing single colonies by matrix-assisted laser desorption ionization–time of flight mass spectrometry. Applied and environmental microbiology, 67(11), 5069-5076.
Ferguson, S., McLay, T., Andrew, R. L., Bruhl, J. J., Schwessinger, B., Borevitz, J., & Jones, A. (2022). Species-specific basecallers improve actual accuracy of nanopore sequencing in plants. Plant Methods, 18(1), 1-11.
Freshwater Algae Culture Collection at the Institute of Hydrobiology. Retrieved from http://algae.ihb.ac.cn/english/
Frostegård, Å., Courtois, S., Ramisse, V., Clerc, S., Bernillon, D., Le Gall, F., . . . Simonet, P. (1999). Quantification of bias related to the extraction of DNA directly from soils. Applied and environmental microbiology, 65(12), 5409-5420.
Gao, J., Zhu, J., Wang, M., & Dong, W. (2018). Dominance and growth factors of Pseudanabaena sp. in drinking water source reservoirs, Southern China. Sustainability, 10(11), 3936.
Georgiou, C. D., & Papapostolou, I. (2006). Assay for the quantification of intact/fragmented genomic DNA. Analytical biochemistry, 358(2), 247-256.
Gerber, N. N. (1969). A volatile metabolite of actinomycetes, 2-methylisoborneol. The Journal of antibiotics, 22(10), 508-509.
Gomes, A. M. d. A., & Lürling, M. (2015). Temperature effect on exploitation and interference competition among Microcystis aeruginosa, Planktothrix agardhii and, Cyclotella meneghiniana. The Scientific World Journal, 2015.
Guo, W., Jiang, L., Bhasin, S., Khan, S. M., & Swerdlow, R. H. (2009). DNA extraction procedures meaningfully influence qPCR-based mtDNA copy number determination. Mitochondrion, 9(4), 261-265.
Hayden, R., Gu, Z., Ingersoll, J., Abdul-Ali, D., Shi, L., Pounds, S., & Caliendo, A. (2013). Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus. Journal of clinical microbiology, 51(2), 540-546.
Hindson, C. M., Chevillet, J. R., Briggs, H. A., Gallichotte, E. N., Ruf, I. K., Hindson, B. J., . . . Tewari, M. (2013). Absolute quantification by droplet digital PCR versus analog real-time PCR. Nature methods, 10(10), 1003-1005.
Hoson, T. (1992). Growth characteristics of the musty odor producing alga, Oscillatoria tenuis. Water Science and Technology, 25(2), 177-184.
Huisman, J., Codd, G. A., Paerl, H. W., Ibelings, B. W., Verspagen, J. M., & Visser, P. M. (2018). Cyanobacterial blooms. Nature Reviews Microbiology, 16(8), 471-483.
Jorgensen, B., Cohen, Y., & Des Marais, D. (1987). Photosynthetic action spectra and adaptation to spectral light distribution in a benthic cyanobacterial mat. Applied and environmental microbiology, 53(4), 879-886.
Juttner, F., & Watson, S. B. (2007). Biochemical and ecological control of geosmin and 2-methylisoborneol in source waters. Applied and environmental microbiology, 73(14), 4395-4406.
Köhler, J. (1993). Growth, production and losses of phytoplankton in the lowland River Spree. I. Population dynamics. Journal of plankton research, 15(3), 335-349.
Kemp, B. M., Winters, M., Monroe, C., & Barta, J. L. (2014). How much DNA is lost? Measuring DNA loss of short-tandem-repeat length fragments targeted by the PowerPlex 16® system using the Qiagen MinElute purification kit. Human biology, 86(4), 313-329.
Kim, Gwan, T., Jeong, S.-Y., & Cho, K.-S. (2014). Comparison of droplet digital PCR and quantitative real-time PCR for examining population dynamics of bacteria in soil. Applied microbiology and biotechnology, 98, 6105-6113.
Kim, C., Lee, S. I., Hwang, S., Cho, M., Kim, H.-S., & Noh, S. H. (2014). Removal of geosmin and 2-methylisoboneol (2-MIB) by membrane system combined with powdered activated carbon (PAC) for drinking water treatment. Journal of Water Process Engineering, 4, 91-98.
Kim, T.-K., Moon, B.-R., Kim, T., Kim, M.-K., & Zoh, K.-D. (2016). Degradation mechanisms of geosmin and 2-MIB during UV photolysis and UV/chlorine reactions. Chemosphere, 162, 157-164.
Klymus, K. E., Merkes, C. M., Allison, M. J., Goldberg, C. S., Helbing, C. C., Hunter, M. E., . . . Monroe, E. M. (2020). Reporting the limits of detection and quantification for environmental DNA assays. Environmental DNA, 2(3), 271-282.
Koepfli, C., Nguitragool, W., Hofmann, N. E., Robinson, L. J., Ome-Kaius, M., Sattabongkot, J., . . . Mueller, I. (2016). Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR). Scientific reports, 6(1), 39183.
Kojabad, A. A., Farzanehpour, M., Galeh, H. E. G., Dorostkar, R., Jafarpour, A., Bolandian, M., & Nodooshan, M. M. (2021). Droplet digital PCR of viral‎ DNA/RNA, current progress, challenges, and future perspectives. Journal of Medical Virology, 93(7), 4182-4197.
Konopka, A. (1981). Influence of temperature, oxygen, and pH on a metalimnetic population of Oscillatoria rubescens. Applied and environmental microbiology, 42(1), 102-108.
Kromkamp, J., Botterweg, J., & Mur, L. R. (1988). Buoyancy regulation in Microcystis aeruginosa grown at different temperatures. FEMS Microbiology Ecology, 4(3-4), 231-237.
Kujbida, P., Hatanaka, E., Campa, A., Colepicolo, P., & Pinto, E. (2006). Effects of microcystins on human polymorphonuclear leukocytes. Biochemical and Biophysical Research Communications, 341(1), 273-277.
Kurmayer, R., Dittmann, E., Fastner, J., & Chorus, I. (2002). Diversity of microcystin genes within a population of the toxic cyanobacterium Microcystis spp. in Lake Wannsee (Berlin, Germany). Microbial ecology, 107-118.
Lürling, M. (2006). Effects of a surfactant (FFD-6) on Scenedesmus morphology and growth under different nutrient conditions. Chemosphere, 62(8), 1351-1358.
Lürling, M., Eshetu, F., Faassen, E. J., Kosten, S., & Huszar, V. L. (2013). Comparison of cyanobacterial and green algal growth rates at different temperatures. Freshwater Biology, 58(3), 552-559.
Lalezary, S., Pirbazari, M., & McGuire, M. J. (1986). Oxidation of five earthy‐musty taste and odor compounds. Journal‐American Water Works Association, 78(3), 62-69.
Lee, S.-Y., Bollinger, J., Bezdicek, D., & Ogram, A. (1996). Estimation of the abundance of an uncultured soil bacterial strain by a competitive quantitative PCR method. Applied and environmental microbiology, 62(10), 3787-3793.
Lee, S., Jang, M. H., Kim, H. S., Yoon, B. D., & Oh, H. M. (2000). Variation of microcystin content of Microcystis aeruginosa relative to medium N: P ratio and growth stage. Journal of applied microbiology, 89(2), 323-329.
Li, Z., Hobson, P., An, W., Burch, M. D., House, J., & Yang, M. (2012). Earthy odor compounds production and loss in three cyanobacterial cultures. Water Research, 46(16), 5165-5173.
Lin, B., Hui, J., & Mao, H. (2021). Nanopore technology and its applications in gene sequencing. Biosensors, 11(7), 214.
Lin, T.-F., Wong, J.-Y., & Kao, H.-P. (2002). Correlation of musty odor and 2-MIB in two drinking water treatment plants in South Taiwan. Science of the total environment, 289(1-3), 225-235.
Lloyd, S. W., Lea, J. M., Zimba, P. V., & Grimm, C. C. (1998). Rapid analysis of geosmin and 2-methylisoborneol in water using solid phase micro extraction procedures. Water Research, 32(7), 2140-2146.
Lorenz, M. G., & Wackernagel, W. (1994). Bacterial gene transfer by natural genetic transformation in the environment. Microbiological reviews, 58(3), 563-602.
Ma, L., Wang, C., Li, H., Peng, F., & Yang, Z. (2018). Degradation of geosmin and 2-methylisoborneol in water with UV/chlorine: influencing factors, reactive species, and possible pathways. Chemosphere, 211, 1166-1175.
Maheshwari, Y., Selvaraj, V., Hajeri, S., & Yokomi, R. (2017). Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR. PLoS One, 12(9), e0184751.
Mao, D., Luo, Y., Mathieu, J., Wang, Q., Feng, L., Mu, Q., . . . Alvarez, P. (2014). Persistence of extracellular DNA in river sediment facilitates antibiotic resistance gene propagation. Environmental science & technology, 48(1), 71-78.
Martins, A., & Vasconcelos, V. (2011). Use of qPCR for the study of hepatotoxic cyanobacteria population dynamics. Archives of microbiology, 193, 615-627.
Mez, K., Hanselmann, K., & Preisig, H. R. (1998). Environmental conditions in high mountain lakes containing toxic benthic cyanobacteria. Hydrobiologia, 368(1-3), 1-15.
Mohanty, B., Majedi, S. M., Pavagadhi, S., Te, S. H., Boo, C. Y., Gin, K. Y.-H., & Swarup, S. (2022). Effects of Light and Temperature on the Metabolic Profiling of Two Habitat-Dependent Bloom-Forming Cyanobacteria. Metabolites, 12(5), 406.
Mur, R., Skulberg, O. M., & Utkilen, H. (1999). CYANOBACTERIA IN THE ENVIRONMENT.
Nagler, M., Insam, H., Pietramellara, G., & Ascher-Jenull, J. (2018). Extracellular DNA in natural environments: features, relevance and applications. Applied microbiology and biotechnology, 102, 6343-6356.
Nanopore, O. (2012). Oxford Nanopore announcement sets sequencing sector abuzz. Nature biotechnology, 30(4), 295.
Neilan, B. A., Pearson, L. A., Muenchhoff, J., Moffitt, M. C., & Dittmann, E. (2013). Environmental conditions that influence toxin biosynthesis in cyanobacteria. Environmental microbiology, 15(5), 1239-1253.
Ni, Y., Liu, X., Simeneh, Z. M., Yang, M., & Li, R. (2023). Benchmarking of Nanopore R10. 4 and R9. 4.1 flow cells in single-cell whole-genome amplification and whole-genome shotgun sequencing. Computational and Structural Biotechnology Journal, 21, 2352-2364.
Nielsen, K. M., Bones, A. M., Smalla, K., & van Elsas, J. D. (1998). Horizontal gene transfer from transgenic plants to terrestrial bacteria–a rare event? FEMS microbiology reviews, 22(2), 79-103.
Nielsen, K. M., Johnsen, P. J., Bensasson, D., & Daffonchio, D. (2007). Release and persistence of extracellular DNA in the environment. Environmental biosafety research, 6(1-2), 37-53.
Ogram, A., Sayler, G. S., & Barkay, T. (1987). The extraction and purification of microbial DNA from sediments. Journal of microbiological methods, 7(2-3), 57-66.
Ogram, A. V., Mathot, M. L., Harsh, J. B., Boyle, J., & Pettigrew Jr, C. A. (1994). Effects of DNA polymer length on its adsorption to soils. Applied and environmental microbiology, 60(2), 393-396.
Paerl, H. W., & Huisman, J. (2008). Blooms like it hot. Science, 320(5872), 57-58.
Paerl, H. W., & Otten, T. G. (2013). Harmful cyanobacterial blooms: causes, consequences, and controls. Microbial ecology, 65, 995-1010.
Paul, V. J. (2008). Global warming and cyanobacterial harmful algal blooms. Cyanobacterial harmful algal blooms: state of the science and research needs, 239-257.
Pendleton, P., Wong, S., Schumann, R., Levay, G., Denoyel, R., & Rouquero, J. (1997). Properties of activated carbon controlling 2-methylisoborneol adsorption. Carbon, 35(8), 1141-1149.
Perkins, R., Slavin, E., Andrade, T., Blenkinsopp, C., Pearson, P., Froggatt, T., . . . Luckwell, R. (2019). Managing taste and odour metabolite production in drinking water reservoirs: The importance of ammonium as a key nutrient trigger. Journal of environmental management, 244, 276-284.
Persson, P.-E. (1988). Odorous algal cultures in culture collections. Water Science and Technology, 20(8-9), 211-213.
Pietramellara, G., Ascher, J., Borgogni, F., Ceccherini, M., Guerri, G., & Nannipieri, P. (2009). Extracellular DNA in soil and sediment: fate and ecological relevance. Biology and Fertility of Soils, 45, 219-235.
Poly, F., Chenu, C., Simonet, P., Rouiller, J., & Jocteur Monrozier, L. (2000). Differences between linear chromosomal and supercoiled plasmid DNA in their mechanisms and extent of adsorption on clay minerals. Langmuir, 16(3), 1233-1238.
Prakash, S. K., LeMaire, S. A., Guo, D.-C., Russell, L., Regalado, E. S., Golabbakhsh, H., . . . Coselli, J. S. (2010). Rare copy number variants disrupt genes regulating vascular smooth muscle cell adhesion and contractility in sporadic thoracic aortic aneurysms and dissections. The American Journal of Human Genetics, 87(6), 743-756.
Reynolds, C. S. (2006). The ecology of phytoplankton: Cambridge University Press.
Reynolds, C. S., Jaworski, G., Cmiech, H., & Leedale, G. (1981). On the annual cycle of the blue-green alga Microcystis aeruginosa Kütz. emend. Elenkin. Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 293(1068), 419-477.
Romo, S., Soria, J., Fernández, F., Ouahid, Y., & BARÓN‐SOLÁ, Á. (2013). Water residence time and the dynamics of toxic cyanobacteria. Freshwater Biology, 58(3), 513-522.
Sanders, R., Huggett, J. F., Bushell, C. A., Cowen, S., Scott, D. J., & Foy, C. A. (2011). Evaluation of digital PCR for absolute DNA quantification. Analytical chemistry, 83(17), 6474-6484.
Schindler, D. W. (1977). Evolution of phosphorus limitation in lakes: natural mechanisms compensate for deficiencies of nitrogen and carbon in eutrophied lakes. Science, 195(4275), 260-262.
Sedlak, R. H., Kuypers, J., & Jerome, K. R. (2014). A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples. Diagnostic microbiology and infectious disease, 80(4), 285-286.
Shanks, O., Sivaganesan, M., Peed, L., Kelty, C., Blackwood, A., Greene, M., . . . Kinzelman, J. (2012). Inter-laboratory general fecal indicator quantitative real-time PCR methods comparison study. Environ Sci Technol, 46(2), 945-953.
Shen, Q., Shimizu, K., Miao, H., Tsukino, S., Utsumi, M., Lei, Z., . . . Fujimoto, N. (2021). Effects of elevated nitrogen on the growth and geosmin productivity of Dolichospermum smithii. Environmental Science and Pollution Research, 28, 177-184.
Singh, R., Varshney, K., & Rani, S. (1985). Adsorption thermodynamics of carbofuran on sandy clay loam and silt loam soils. Ecotoxicology and environmental safety, 10(3), 309-313.
Sivaganesan, M., Siefring, S., Varma, M., & Haugland, R. A. (2011). MPN estimation of qPCR target sequence recoveries from whole cell calibrator samples. Journal of microbiological methods, 87(3), 343-349.
Stackebrandt, E., & Goebel, B. M. (1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International journal of systematic and evolutionary microbiology, 44(4), 846-849.
Stewart, W. (1980). Some aspects of structure and function in n fixing cyanobacteria. Annual Reviews in Microbiology, 34(1), 497-536.
Su, M., Suruzzaman, M., Zhu, Y., Lu, J., Yu, J., Zhang, Y., & Yang, M. (2021). Ecological niche and in-situ control of MIB producers in source water. Journal of Environmental Sciences, 110, 119-128.
Tabachek, J.-A. L., & Yurkowski, M. (1976). Isolation and identification of blue-green algae producing muddy odor metabolites, geosmin, and 2-methylisoborneol, in saline lakes in Manitoba. Journal of the Fisheries Board of Canada, 33(1), 25-35.
Taylor, S. C., Laperriere, G., & Germain, H. (2017). Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data. Scientific reports, 7(1), 2409.
Tonk, L., Visser, P. M., Christiansen, G., Dittmann, E., Snelder, E. O., Wiedner, C., . . . Huisman, J. (2005). The microcystin composition of the cyanobacterium Planktothrix agardhii changes toward a more toxic variant with increasing light intensity. Applied and environmental microbiology, 71(9), 5177-5181.
Torti, A., Jørgensen, B. B., & Lever, M. A. (2018). Preservation of microbial DNA in marine sediments: insights from extracellular DNA pools. Environmental microbiology, 20(12), 4526-4542.
Tsai, H. L., Wong, Y. G., & Ho, W. H. (2004). Determination of Aqueous Butachlor Using Solid‐phase Microextraction and Gas Chromatography. Journal of the Chinese Chemical Society, 51(5A), 955-961.
Tuji, A., & Niiyama, Y. (2018). Two new Pseudanabaena (Cyanobacteria, Synechococcales) species from Japan, Pseudanabaena cinerea and Pseudanabaena yagii, which produce 2‐methylisoborneol. Phycological Research, 66(4), 291-299.
Uherkovich, G. (1966). Das Leben der Tisza. XXVII. Zur Frage der Potamolimnologie und des Potamoplanktons. Acta Biologica Szeged, 12, 55-66.
Van der Westhuizen, A., & Eloff, J. (1983). Effect of culture age and pH of culture medium on the growth and toxicity of the blue-green alga Microcystis aeruginosa. Zeitschrift für Pflanzenphysiologie, 110(2), 157-163.
Van Liere, L., & Mur, L. R. (1980). Occurrence of Oscillatoria agardhii and some related species, a survey. Paper presented at the Hypertrophic Ecosystems: SIL Workshop on Hypertrophic Ecosystems held at Växjö, September 10–14, 1979.
Vuillemin, A., Horn, F., Alawi, M., Henny, C., Wagner, D., Crowe, S. A., & Kallmeyer, J. (2017). Preservation and significance of extracellular DNA in ferruginous sediments from Lake Towuti, Indonesia. Frontiers in Microbiology, 8, 1440.
Wagner, C., & Adrian, R. (2009). Cyanobacteria dominance: quantifying the effects of climate change. Limnology and Oceanography, 54(6part2), 2460-2468.
Wang, Y., Zhao, Y., Bollas, A., Wang, Y., & Au, K. F. (2021). Nanopore sequencing technology, bioinformatics and applications. Nature biotechnology, 39(11), 1348-1365.
Wang, Z., & Li, R. (2015). Effects of light and temperature on the odor production of 2-methylisoborneol-producing Pseudanabaena sp. and geosmin-producing Anabaena ucrainica (cyanobacteria). Biochemical Systematics and Ecology, 58, 219-226.
Whipple, G. M. (1927). How to study effectively (Vol. 10): Public School Publishing Company.
Wilson, A. E., Sarnelle, O., & Tillmanns, A. R. (2006). Effects of cyanobacterial toxicity and morphology on the population growth of freshwater zooplankton: Meta‐analyses of laboratory experiments. Limnology and Oceanography, 51(4), 1915-1924.
Wood, S. A., Heath, M. W., Holland, P. T., Munday, R., McGregor, G. B., & Ryan, K. G. (2010). Identification of a benthic microcystin-producing filamentous cyanobacterium (Oscillatoriales) associated with a dog poisoning in New Zealand. Toxicon, 55(4), 897-903.
Yang, J., Zhang, N., Lv, J., Zhu, P., Pan, X., Hu, J., . . . Li, H. (2020). Comparing the performance of conventional PCR, RTQ-PCR, and droplet digital PCR assays in detection of Shigella. Molecular and cellular probes, 51, 101531.
Yang, R., Abraham, S., Gardner, G., Ryan, U., & Jacobson, C. (2017). Prevalence and pathogen load of Campylobacter spp., Salmonella enterica and Escherichia coli O157/O145 serogroup in sheep faeces collected at sale yards and in abattoir effluent in Western Australia. Australian Veterinary Journal, 95(5), 143-148.
Yuan, K., Wang, X., Chen, X., Zhao, Z., Fang, L., Chen, B., . . . Chen, B. (2019). Occurrence of antibiotic resistance genes in extracellular and intracellular DNA from sediments collected from two types of aquaculture farms. Chemosphere, 234, 520-527.
Zhang, T., Zheng, L., Li, L., & Song, L. (2016). 2-Methylisoborneol production characteristics of Pseudanabaena sp. FACHB 1277 isolated from Xionghe Reservoir, China. Journal of Applied Phycology, 28, 3353-3362.
Zhao, C., Shao, N., Yang, S., Ren, H., Ge, Y., Feng, P., . . . Zhao, Y. (2019). Predicting cyanobacteria bloom occurrence in lakes and reservoirs before blooms occur. Science of the total environment, 670, 837-848.
Zhou, S., Shao, Y., Gao, N., Zhu, S., Li, L., Deng, J., & Zhu, M. (2014). Removal of Microcystis aeruginosa by potassium ferrate (VI): Impacts on cells integrity, intracellular organic matter release and disinfection by-products formation. Chemical Engineering Journal, 251, 304-309.
中華日報. (2019). 運河盲段水質不良 尋改善良策.
今日新聞. (2023). 水情持續告急！曾文、阿公店水庫見底.
自由時報. (2013). 愛河泛紅 初判藻類或底泥造成. 自由時報.
李, 思., & 莊以光. (2010). DNA 定序技術之演進與發展. J Biomed Lab Sci, 22(2), 49.
李, 紹. (2017). 利用生物降解水中 MIB 可行性之研究: 降解菌分離及降解效率比較.
林, 財., 顏宏愷, 林秀蓮, 邱宜亭, & 李紹鈺. (2016). 公共給水有害藻類及代謝物監測與緊急應變處理技術之研究.
邱, 宜., 黃良銘, & 林財富. (2020). 分子生物監測技術在有害藻類及水質監測管理的應用. 中國土木水利工程學刊, 32(3), 303-309.
姚, 若. (2021). 兩種分子生物方法監測水源中產2-MIB藍綠菌之開發及應用.
張, 艳., 李德祥, 张凤枰, & 邹昌健. (2021). 固相微萃取-气质联用法测定鱼肉中土臭味素和 2-甲基异莰醇. 渔业科学进展, 42(5), 167-175.
陳, 怡. (2019). 以主成份分析評估台灣亞熱帶德基水庫水質變化與藻類變遷關係. Journal of Taiwan Agricultural Engineering, 65(3).
童, 淑., 徐孟鴻,楊士賢,林財富. (2002). 利用氧化劑去除原水中 2-MIB 之研究. 第二十七屆廢水處理技術研討會論文集.
黃, 富., 李俊福, 古煥林, 賴允偉, 陳淳圓, 吳承恩, . . . 劉彥君. (2005). 以多變量統計分析探討水庫水質優養化最適指標.
薛, 志. 賴. 張. 王. 張. (2019). 傳統淨水處理程序因應原水中 微囊藻毒處理策略之研究.
謝, 汶. (2013). 2-MIB 與 Geosmin 在土壤-水系統分佈及淨水程序之處理研究.