本研究成功開發出具可大量分析、穩定度佳及易操作特色之全自
動糞便潛血定量免疫分析儀；在硬體方面利用滾珠螺桿、步進馬達、
馬達驅動器、高精準度移液系統、輸送帶以及四軸控制器設計出全自
動糞便潛血定量免疫分析儀主體， 並使用可程式控制器(Programmable Logic Controller, PLC)撰寫出檢測控制程式；軟體部分為光學檢測模組並裝載Advanced RISC Machine (ARM)開發板、Universal Serial Bus (USB)攝影機、LED 光源、LCD 觸控式液晶顯示器、整合式電路及光學暗房。在檢測程式方面使用Python 整合模組OpenCV、NumPy 及Matplotlib 撰寫出影像處理程式，再以Python 模組Sqlite 紀錄資訊並由觸控式螢幕顯示檢測資訊。最後使用Raspberry pi (樹莓派)將硬體、軟體做整合，開發出全自動糞便潛血全自動定量免疫分析儀。由研究結果可發現，開發之全自動糞便潛血定量免疫分析儀可精準達到完整檢測自動化之目的，並在滴入五滴孔卡匣上之糞便潛血檢測試劑下，能正確建立出線性度極佳之濃度與灰階值校正曲線，作為量化分析依據，該曲線決定係數可達0.95，在重複實驗下仍可保持良好的精準度。

This thesis presents a highly sensitive and stable automated immunoanalyzer system for fecal occult blood tests (FOB). The inspection is based on immunochromatographic assay, which is widely applied to fecal occult blood detection. Combined with the immunochromatographic strip, the automated immunoanalyzer system is able to detect and calculate the concentration of sample. The system, designed by SolidWorks, is composed of hardware and software. The analyzing programs include a main program, QR code, and image processing program compiled by Python.

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