在本論文中，我們製備了以螢光量子點(QD)修飾的DNA分子梳並將其應用在具備分子感應及偵測的一維奈米陣列上。於陣列上，我們使用具有目標針對性的量子點以及螢光共振能量轉移(FRET)的技術，在捕捉及檢測到目標分子時，以FRET來說明捕捉的完成及檢測的結果。我們由實驗上證實利用這樣DNA分子梳的設計不僅能夠藉捕捉更多的目標分子來提高FRET的訊號，而且還能藉獨特的雙重激發機制來進一步提高FRET效率。

關鍵字：DNA分子梳、螢光共振能量轉移、一維生物奈米感測器。

In this thesis, we prepare molecular comb of DNA having functionalized luminescent quantum dots (QDs) along its backbone and turn this setup into a one-dimensional nanoarray for molecular sensing and detection. The detection is realized by fluorescence resonance energy transfer (FRET) in which target-specific QDs are used to capture desired ssDNA molecules followed by a transmission of the fluorescence energy in between to signal the binding. We demonstrate that this DNA-comb biosensor is not only capable of amplifying FRET signals by capturing more targeted molecules, but also able to raise the FRET efficiency due to the unique double excitation mechanism resulting from the one-dimension geometry.

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