本實驗室過去以外消旋Naproxen三氟乙硫酯為基質，利用Lipase MY配合三辛基胺為鹼觸媒之動態動力分割反應已有相當好的成果，但是在長時間反應時，酵素會漸漸失活而降低反應速率。許多文獻報導以聚乙烯亞胺(PEI, polyethyleneimine)修飾酵素，可提升酵素的反應性及穩定性。因此，本論文之研究目的在於使用PEI修飾後的固定化酵素，配合有機鹼1,8-Diazabicyclo[5.4.0]undec-7-ene(1,5-5) (DBU)進行外消旋Naproxen三氟乙硫酯之動態動力分割。

論文中嘗試改變PEI修飾固定化酵素條件，並配合動力分割進行條件篩選，發現經由5 % PEI水溶液、攪拌60分鐘處理的固定化酵素，在動力分割方面約提高了2倍酵素活性，故進一步選擇此條件下處理的固定化酵素以進行外消旋Naproxen三氟乙硫酯之動態動力分割反應。利用實驗值擬合出動力參數，進而比較理論值與實驗值，推測反應進行時應考慮產物抑制、鹼水解之影響。

在添加10 mM DBU進行分割時，發現酵素反應性提高約10倍，顯示有機鹼會對經由PEI修飾後的固定化酵素有活化效果。

A dynamic kinetic resolution process of using racemic Naproxen trifluoroethyl thioester as the substrate as well as Lipase MY and trioctylamine as the biocatalyst and recemization catalyst, respectively, has been developed. However, enzyme deactivation was found for a long period time of reaction. Literatures have reported that PEI (polyethyleneimine) could be used to modify enzymes in order to increase enzyme activity and stability. Therefore, the purpose of this research is aimed to develop a dynamic kinetic resolution process of using (R, S)-Naproxen trifluoroethyl thioester as the substrate. Immobilized lipase modified with 5 % PEI solution with agitation for 60 minutes can increase two folds of enzyme activity. By using this condition for the dynamic kinetic resolution process, the kinetic parameters were estimated and experimented data were compared with theoretical results by further considering the effect of product inhibition and hydrolysis by the organic base. Besides, the activity of the modified enzyme increased ten times when adding 10 mM of DBU during the dynamic kinetic resolution.

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