因為現今有許多細胞表面性質的病變是造成許多疾病的病因，而且當細胞分裂時，細胞表面的性質也會隨著分裂階段的不同而有所改變，因此針對細胞表面從事相關的研究以及檢測便有它的重要性。
本論文利用黏彈式原子力顯微鏡對活體PC12類神經細胞量測其細胞表面的機械性質。黏彈式原子力顯微鏡是以載台振盪帶動探針頭振盪，並以動態壓痕的方式使細胞在量測時能夠表現出其黏彈性質。而在液面下黏彈性材料的動態壓痕彈性模數理論中則以佛格脫固體(Voigt solid)模型來表示細胞的黏彈性質，使理論分析也能夠以黏彈性質來表現。
由實驗可以得到細胞表面形貌、探針頭振盪振幅以及探針頭振盪振幅與相位落後餘弦此三種實驗結果，再將其代入液面下黏彈性材料的動態壓痕彈性模數理論中便能得到其楊氏模數的定量值大小。此外，本論文還利用表面形貌以及探針頭的幾何關係來修正正向接觸投影面積，不但可以去除形貌對楊氏模數的影響，還可以排除量測過程中錯誤的資訊。
最後我們所推得的楊氏模數不論在尺度上還是隨壓深不同而改變的趨勢上皆與正常情況相符，但是其值還是有受到其他表面形貌效應的影響，這說明在本研究中影響楊氏模數的因素並不只有一個。

In this study, we used VE-AFM to examine mechanical properties of PC12 neuron-like cells. Cells displayed their viscoelasticity by the way of dynamic indentations in which the tip of VE-AFM oscillated from the oscillation of the specimen holder. It also displayed viscoelasticity in elastic modulus theory, in which a Voigt solid model was employed, of dynamic indentations for viscoelastic materials in liquid.
We got the experiment data of topography, tip oscillation amplitude, and the cosine of the tip oscillation amplitude and the phase lag, and we got the Young’s modulus after putting the data into the elastic modulus theory. Besides, we employed the relations between topography and the tip geometry to eliminate the effect of topography and exclude errors in experiments.
The Young’s modulus we got was not only in same order but also in same trend in different indentation depth with true situation, but it was still affected by other topographical effects.

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