在本論文中，主要藉由飛秒雷射系統結合非線性光學之多光子激發效應(multiphoton excitation)的技術來進行微透鏡之製作。由於多光子激發效應能將有效激發區域侷限於一極小的體積之中，因此可用於高精緻三維結構的製作。首先藉由繪圖軟體設計微透鏡的直徑、高度、曲率等參數，並計算理論數值孔徑(numerical aperture，NA)、焦距以及微透鏡最佳聚焦點的大小。實驗過程中，分別使用三種材料來進行微透鏡製作之實驗。第一種配方使用光學樹脂Norland Optical Adhesive 81作為加工材料，透過光聚合反應，製作4×4的微透鏡陣列。第二種配方使用蛋白質材料，牛血清蛋白(bovine serum albumin，BSA)，透過交聯反應機制製作微透鏡。第三種配方以BSA作為支撐結構的材料並摻雜少許石墨烯量子點(graphene oxide quantum dots，GOQDs)製作微透鏡。為了檢驗加工出來的透鏡品質，初步透過掃描式電子顯微鏡(scanning electron microscope)檢視表面形貌並透過物鏡嘗試將微透鏡之聚焦點影像成像於感光耦合元件(charged-coupled device)上。從檢測結果顯示，BSA以及BSA摻雜GOQDs的實驗結果，其表面都有些許孔洞存在，若要用於微透鏡製作較不適合。以上述光學樹脂製作之透鏡，其表面緻密程度優於BSA配方和BSA摻雜GOQDs配方，光線在透鏡內部傳播較不會受到孔洞所造成的散射影響，在微透鏡的製作上相對較為理想。

In this thesis, using the femtosecond laser microfabrication system via a nonlinear optical effect called multi-photon excitation (MPE), a microlens can be fabricated. Owing to the fact that the excitation region can be confined to a tiny focal volume, sophisticated three-dimensional structures can be realized through MPE. In the thesis, the diameter, height and curvature of the microlens (plano-convex) are designed using drawing software. Furthermore, the theoretical numerical aperture (NA), focal length and focusing size of the microlenses were computed. In the study, three kinds of materials were used for microlens fabrication. In the first formulation, a 4×4 microlens array was fabricated using the optical resin, Norland Optical Adhesive 81, through photo-polymerization. In the second formulation, microlenses were processed using a protein, bovine serum albumin (BSA), through the crosslinking mechanism. In the third formulation, the BSA-dominant structure doped with the graphene oxide quantum dots (GOQDs) was tested tentatively. In order to check the morphology and focusing quality of the fabricated microlens, the morphology of the fabricated microlens was observed using a scanning electron microscope, and the focusing spot was imaged on the charged-couple device via an objective. According to the results of the experiment, the consequences of BSA and BSA doped with GOQDs lead to some porous holes on the surface.　This means that these two materials are not really suitable in microlens fabrication. The surface of the optical resin based microlens is more compact than the two other materials. When the light passes through the medium of the optical resin based material, the optical path is thus less affected by scattering due to the porous morphology. Therefore, an optical resin based material is relatively ideal in the production of a microlens.

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