為了在成像平台上傳輸斑馬魚幼魚，長期以來一直透過使用鑷子或機械泵浦進行傳輸。這些方法在運輸過程中對斑馬魚幼魚的脆弱身體會產生不利的損害。為了解決這個問題，在本論文中，設計一種在微流體環境中運輸斑馬魚幼魚的光驅動技術。針對斑馬魚幼魚的視覺運動行為反應通過電腦動畫產生移動光柵(條紋)控制，用於在微流體內運輸。據觀察結果討論，在1.5Hz的最佳光柵頻率和1：1的光柵寬度比下，授精5天後斑馬魚幼魚可分別在0.63和2.49s的最小和最大時間段內運輸。這項技術可用於在微流體環境內斑馬魚幼魚以及斑馬魚核心設施的多自動運輸。此外，也利用此技術進行斑馬魚毒性檢測觀察其行為差異性作為判斷此物質對於斑馬魚幼魚是否有影響，由於目前乙醇為已知的發展毒性物質，對於胚胎生長有明顯影響性，在本論文中採用不同濃度乙醇作為待測液體，據觀察結果討論，在體積濃度 1.5 % (vol/vol) 下其身體長度與控制組差異達23 % 此過程需使用成像系統進行觀察，此外在行為觀測中其傳輸時間差異達54 %，在觀測中結果中，乙醇對於行為影響可以明顯被觀測出來，因此光驅動技術不僅可以作為斑馬魚於微流道平台中之傳輸用途也可作為毒性檢測中之初步的觀察以判斷此物質對於斑馬魚是否有害。

Transferring the zebrafish larvae on an imaging platform has long been performed manually by the use of forceps or through mechanical pumping. These methods induce detrimental damages to the fragile bodies of zebrafish larvae during the transportation. To address this issue, in this work we are devising a light driven technique to transport zebrafish larvae within a microfluidic environment. In particular, an optomotor behavioral response of the zebrafish larvae was controlled through the computer animated moving gratings for their transportation within a microfluidics chamber. It was observed that with an optimum grating frequency of 1.5 Hz and a grating width ratio of 1:1, a 5 days-post fertilization zebrafish larva can be transported within minimum and maximum time periods of 0.63 and 2.49 s, respectively. This proposed technique can be utilized towards multi-automatic transportation of zebrafish larvae within the microfluidic environment as well as the zebrafish core facility. In addition to it, this platform was used to realize the behavioral differences of juvenile larvae to realize the effect of ethanol toxicity.

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