急性肝胰腺壞死症 (Acute hepatopancreatic necrosis disease，AHPND) 在早期蝦苗養殖時期所造成100%死亡率，導致巨大的經濟損失，其病原體是一株攜帶毒素基因PirA/B特殊質體的副溶血弧菌 (Vibrio parahaemolyticus，VP) 。先前本實驗室已建立初步AHPND的致病機轉，但對於病蝦免疫反應尚未解析。在本研究中，主要針對蝦隻抵抗細菌的免疫路徑Toll及IMD路徑在感染AHPND病蝦的變化情形進行探討。其結果顯示在VP聚集且形成菌落的病蝦胃中，Toll及IMD所調控抗菌肽的表現量會因AHPND感染而被抑制，而此現象也可在蝦隻主要免疫組織的血球細胞發現。然而，在AHPND主要病灶組織的肝胰腺中，這些基因大部分卻是過度活化，特別是IMD免疫路徑所調控的基因群。本研究後續使用dsRNA默化IMD路徑中的關鍵訊息傳遞因子Relish，抑制IMD路徑的活化以探討IMD訊息傳遞路徑在AHPND所扮演的角色。從AHPND所導致死亡率結果顯示抑制Relish的組別明顯高於控制組，推測IMD免疫路徑的過度活化是由蝦體本身所驅動的免疫反應，藉此對抗AHPND病原體的入侵。本研究首次觀測蝦體免疫分子於AHPND感染過程中之變化，有助於未來AHPND病原體與宿主互動脈絡之建立。

That acute hepatopancreatic necrosis disease (AHPND) can cause up to 100% mortality in post-larvae shrimp makes it a costly problem for the shrimp industry. The etiology of AHPND is Vibrio parahaemolyticus (VP) which has a unique plasmid containing toxic genes, PirA and PirB. Our laboratory has already reported the basic pathogenesis of AHPND. However, the immune response of AHPND-infected shrimp remains unknown. In this study, we focus on two immune pathways, Toll and IMD, in shrimp during AHPND infection. In AHPND-infected shrimp, Toll- and IMD-regulated antimicrobial peptides (AMPs) were suppressed in the stomach (where VP colonized) and in hemocytes. However, in hepatopancreas (location with most profound lesion due to AHPND), these immune genes were significantly upregulated, especially IMD-regulated immune genes. To investigate the role of the IMD pathway in AHPND-infected shrimp, dsRNA was used to silence Relish, the key factor of the IMD pathway. Mortality was significantly higher in the Relish silencing group than the control group. We inferred that overexpression of the IMD pathway was caused by a host immune response against AHPND. This was apparently the first investigation of expression of immune genes in shrimp during AHPND infection. These results will provide the basis for future studies to elucidate interactions between the host immune network and AHPND.

賴虹喬，引發蝦隻急性肝胰腺壞死病之副溶血弧菌特性及辨識病原體之唐氏綜合症細胞黏附分子異構型抗性潛力應用分析，國立成功大學生物科技研究所碩士論文，2015。
蘇美安，雷帕黴素標靶蛋白訊息傳遞路徑在白點症病毒之致病機轉中所扮演之角色，國立成功大學生物科技研究所碩士論文，2013。
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