近年來，化學動力學治療被廣泛研究並應用於癌症治療上，雖然化學動力學治 療已被證實可以抑制腫瘤細胞的生長，但腫瘤細胞內源性過氧化氫濃度仍不足以使 芬頓催化劑提供理想的治療效果，為此，本研究設計了一種具有原子分散金之零價 銅奈米立方體，稱為金銅奈米立方體(AuxCu1-x)，透過原子分散金增強零價銅之連續 性類氧化酶與類芬頓反應 (O2→H2O2→•OH)，而大量生成具高細胞毒性之羥基自由基，以有效增強化學動力學治療。

首先，合成零價銅奈米立方體，接著透過伽凡尼取代藉由控制金鹽的體積製備不同金銅莫耳比且具原子分散金之AuxCu1-x (x = 0.02, 0.05, 0.1, 0.5)，並於表面包覆硬脂酸以避免材料提早與氧氣進行反應。當材料通過胞吞作用進入腫瘤細胞時，硬脂酸 會與細胞膜融合而釋出裡面的AuxCu1-x，使零價銅開始氧化並降解為銅離子(Cu0 → Cu+ + e- → Cu2+ + e-, Cu0 → Cu2+ + 2e-)，同時丟出電子還原氧氣生成過氧化氫(O2 + 2H+ + 2e- → H2O2)，並透過銅離子與過氧化氫之類芬頓反應產生羥基自由基(Cu+ + H2O2 → Cu2+ + •OH + OH-)，促使腫瘤細胞凋亡。實驗結果證實，Au0.02Cu0.98生成 •OH的 反應效率最好。DFT計算結果表明，在Au0.02Cu0.98上O2兩次被氫化 (O2→OOH, OOH→H2O2) 的活化能 (路徑1：0.76 eV, 0.72 eV、路徑2：0.96 eV, 1.08 eV) 均比Cu NCs (1.31 eV, 1.31 eV) 低，因此Au0.02Cu0.98相較於Cu NCs具有更快的H2O2 生成速率，使Au0.02Cu0.98具有更好的化學動力學治療療效。最後，材料在進入腫瘤細胞後會完全降解為離子，具生物可降解性。

Chemodynamic therapy (CDT) is limited by endogenous H2O2 levels. To improve the insufficient therapeutic efficacy, this study reports a degradable zero-valent copper nanocubes (AuxCu1-x) with atomically dispersed golds, which exhibited cascade oxidase- and Fenton-like catalytic activities, where Au0.02Cu0.98 possess the most efficient catalytic activity. AuxCu1-x synthesized through galvanic replacement was subsequently encapsulated with stearic acid (SA) to avoid decomposition outside the cancer cells. After AuxCu1-x@SA endocytosis into cancer cells, AuxCu1-x will be released, which possessed single-atom golds assist Cu0 in activating O2 to H2O2 and simultaneously generated Cu+ for subsequently augment Fenton-like reactions for CDT. The results show that Au0.02Cu0.98 could generate larger amount of H2O2 and ·OH than Cu NCs due to single-atom golds assistance. In addition, Au0.02Cu0.98 had the best cancer treatment efficacy and finally AuxCu1-x degraded.

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