調控哺乳類動物和果蠅肌肉發育的基因網絡是高度保留的(conversed)，因此可藉由遺傳學方式來探討果蠅骨骼肌發育過程以利於了解哺乳類動物肌肉的發育。本論文探討一個尚未被研究過的CG 基因。CG 基因在果蠅胚胎發育中期，除了會表現在骨骼肌前驅細胞中，也會表現在平滑肌前驅細胞中。為了要了解CG 在骨骼肌發育過程中所扮演的角色，利用 FRT-FLP 重組技術移除CG 並觀察CG 突變時的表現型態，結果發現，跟 wide type 相比，wide type 在胚胎發育後期會有肌纖維的形成，但
CG 突變時的表現型態卻沒有肌纖維的形成，反而呈現未融合的細胞狀態，因此將CG 基因命名為round - up 基因，推測round - up 基因可能跟調控骨骼肌的細胞融合有關。此外，也觀察round - up 基因過量表現時的肌肉發育形態，結果發現，在胚胎發育後期，肌纖維的數量有異常現象而且平滑肌也不能把中腸正常的收縮成四個腔室。為了探討CG 基因表現如何被調控，選殖round - up 基因的候選調控區域放置於報導基因綠螢光基因( GFP )前並建立帶有此片段的轉殖果蠅株，收集其胚胎並利用非螢光免疫染色觀察，結果發現，此候選調控區域表現形態跟內源性
CG 基因表現形態一致，表示此候選調控區域為round - up 基因的調控序列。

Somatic muscle development in Drosophila is analogous to mammals. To dissect Drosophila muscle development by genetics helps understand mammalian muscle development. I analyzed the role of an uncharacterized CG gene in mesoderm development during Drosophila embryogenesis. During Drosophila mid-embryogenesis, expression of CG was in the somatic muscle precursor cells and the visceral muscle precursor cells. In order to characterize the role of the CG gene in Drosophila somatic muscle development, I used FRT-FLP recombination to remove CG and examined phenotype of mutant CG embryos. Wild type embryos form muscle fibers at late embryogenesis, but I found that mutant embryos of the CG gene didn’t form muscle fibers. Instead, the founder cells remained unfused and appear in a rounded shape. I therefore named the gene round up. It implied that the gene round up may be involved in myoblast fusion in somatic muscle development. I then overexpressed round-up in mesoderm and found that embryos with round-up overexpression is defecting in the somatic muscle and abnormal constrictions in the visceral muscle during embryogenesis. To understand how CG gene expression is regulated, I cloned a candidate cis-regulatory region of round-up gene upstream a reporter gene GFP, and established transgenic fly carring this construct. I used non-fluorescence immunostaining to examine the expression of GFP which is driven by the candidate cis-regulatory region. I found that the expression pattern of the candidate genomic region was similar to round-up endogenous expression.

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