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研究生：	郭振武 Kuok, Chan-mou
論文名稱：	比生長速率對以鏈球菌醱酵生產玻尿酸之影響 Effect of Specific Growth Rate on Hyaluronic Acid Production by Streptococcus zooepidemicus
指導教授：	陳特良 Chen, Teh-Liang
學位類別：	碩士 Master
系所名稱：	工學院 - 化學工程學系 Department of Chemical Engineering
論文出版年：	2007
畢業學年度：	95
語文別：	中文
論文頁數：	68
中文關鍵詞：	鏈球菌、玻尿酸、醱酵、比生長速率
外文關鍵詞：	fermentation, Streptococcus zooepidemicus, Specific Growth Rate, Hyaluronic Acid
相關次數：	點閱：85 下載：1
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本研究探討在以Streptococcus zooepidemicus醱酵生產玻尿酸的程序中，細胞比生長速率對玻尿酸合成效率之影響。於探討最佳的葡萄糖與酵母萃取物濃度比例的批次實驗中，當比例為2.5時，可得到最大的玻尿酸比產率(YP/X = 0.80)；當比例為1.33時，得到較低的玻尿酸比產率(YP/X = 0.61)。同時，菌體的比生長速率亦隨著此比例改變。吾人在此最佳的比例(2.5)下，控制菌體在不同的比生長速率下生長，以進行玻尿酸生產。實驗結果發現玻尿酸的比產率不會受到菌體比生長速率的影響，而葡萄糖與酵母萃取物之比例才是影響玻尿酸合成效率的主要因素。

In this research, we focused on the effect of specific growth rate on hyaluronic acid production by Streptococcus zooepidemicus. At first, we tried to find out the best ratio of glucose to yeast extract in the medium from batch experiments. The specific productivity of hyaluronic acid would have a maximum value (YP/X = 0.80) when at a ratio of 2.5; and a low value (YP/X = 0.61) would result if a ratio of 1.33. We also observed that the specific growth rate changed with changing the ratio of glucose to yeast extract . Then, we used this best ratio in the fed-batch cultivation. It was found that the specific productivity of hyaluronic acid would not change with changing the specific growth rate, rather, it changed with changing the ratio of glucose to yeast extract.

中文摘要.................................................Ⅰ
英文摘要.................................................Ⅱ
誌謝.....................................................Ⅲ
總目錄...................................................Ⅳ
圖目錄...................................................Ⅷ
表目錄...................................................XI
符號表..................................................XII

第一章	緒論.............................................1
1-1	前言.............................................1
1-2 玻尿酸的發現..........................................2
1-3 玻尿酸的結構..........................................2
1-3-1 一級結構............................................2
1-3-2 二級結構............................................2
1-3-3 三級結構............................................4
1-4 玻尿酸的來源及合成....................................4
1-4-1 玻尿酸的來源........................................4
1-4-2 玻尿酸的合成........................................6
1-5 玻尿酸的黏彈性質......................................6
1-6 玻尿酸的醫療用途......................................9
1-7 玻尿酸應用於藥物輸送系統..............................9
1-7-1 皮膚藥物輸送.......................................10
1-7-2 眼科藥物輸送.......................................10
1-7-3 鼻腔藥物輸送.......................................10
1-7-4 肺部藥物輸送.......................................10
1-7-5 非口服藥物輸送.....................................11
1-7-6 脂質體藥物輸送.....................................11
1-7-7 殖入性藥物.........................................11
1-7-8 基因輸送...........................................13
1-8 醱酵策略.............................................13
1-9 文獻回顧及研究動機...................................14
1-9-1 文獻回顧...........................................14
1-9-2 研究動機...........................................16
第二章　實驗材料與方法...................................18
2-1 實驗菌株與藥品.......................................18
2-1-1 實驗菌株...........................................18
2-1-2 實驗藥品...........................................18
2-2 實驗儀器與裝置.......................................19
2-2-1 實驗儀器...........................................19
2-3 培養基...............................................21
2-4 實驗方法.............................................21
2-4-1 菌株保存...........................................21
2-4-2 菌株活化...........................................23
2-4-3 前培養.............................................23
2-4-4 批次醱酵...........................................23
2-4-5 兩階段批次.........................................23
2-4-6 饋料批次醱酵.......................................25
2-4-7 饋料批次進料模式...................................25
2-4-8 實驗流程圖.........................................28
2-5 分析方法.............................................29
2-5-1 菌體濃度分析.......................................29
2-5-2 玻尿酸濃度分析.....................................31
2-5-3 葡萄糖濃度分析.....................................32
2-5-4 有機酸分析.........................................35
2-5-5 分子量分析.........................................39
第三章　實驗結果與討論...................................41
3-1 批次實驗結果.........................................41
3-2 兩階段批次及饋料批次實驗.............................51
3-2-1 兩階段批次.........................................51
3-2-2 饋料批次實驗.......................................51
第四章　結論.............................................64
參考文獻.................................................65
自述.....................................................68
                                    

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校內：立即公開
校外：2007-07-26公開

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