當神經束受到病變影響產生斷裂時，如果斷裂間隙過大，必須考慮使用神經導管手術進行神經修復。如果可以在神經導管內施加適當之電刺激，一般相信可以使再生神經纖維束更有效地連結。本研究主要目的為探討神經細胞軸突在電場刺激下，對其生長定位與機械性質之影響。
本研究實驗對像為PC-12類神經細胞，方法為針對同一顆細胞做原子力顯微鏡多點定位壓深實驗，並搭配免疫螢光染色。因為為高解析度、低壓深實驗，因此同時考慮原子力顯微鏡真實探針幾何形狀，使用鈍角錐力學模型分析壓深實驗資料，計算出細胞軸突末端視楊氏模數。
實驗結果顯示，施加電場使軸突生長方向逐漸偏向電場方向。視楊氏模數部分，本研究發現細胞軸突末端視楊氏模數與骨架分佈具有正相關性，另外，電場刺激會造成細胞軸突末端骨架重組並導致視楊氏模數增強。當神經細胞受到電場刺激時，會使生長錐上鈣離子濃度增加，造成軸突生長方向偏向負極。另外板足(filopodium)及偽足(lamellpodium)結構上也會受到鈣離子影響，使軸突末端視楊氏模數增加。

The nerve may be fractured due to trauma or disease, if the gap between nerve segments is too far from each other, the use of nerve conduits is often utilized for nerve repair. By applying appropriate electrical stimulation in nerve conduit, it is believed that regeneration of nerve fibers can enhanced. The main purpose of this thesis was to explore outgrowth orientation and mechanical properties of the nerve axons affected by the electrical field stimulation.
The subjects in this study are PC-12 neuron-like cells. Methods for multi-point indentation using atomic force microscope and immunofluorescence image for the same cell. A blunted-pyramid model by considering the real probe geometry was employed to calculate the apparent Young’s modulus of the axon
The results showed that the orientation of axon moves to the cathode progressively. The mechanical properties of axon are highly correlated to the distribution of cytoskeleton. The calcium ion concentration at growth cone may be increased due to electric field and the ultra structure filopodium and lamellpodium might be reorganized and their apparent Young’s modulus were increased.

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