| 研究生: |
李居佑 Li, Chi-Yu |
|---|---|
| 論文名稱: |
快速微流體電化學傳感器用於檢測微量白蛋白尿 Rapid Microfluidic Electrochemical Biosensor for Microalbuminuria detection |
| 指導教授: |
傅龍明
Fu, Lung-Ming |
| 學位類別: |
碩士 Master |
| 系所名稱: |
工學院 - 工程科學系 Department of Engineering Science |
| 論文出版年: | 2020 |
| 畢業學年度: | 108 |
| 語文別: | 中文 |
| 論文頁數: | 83 |
| 中文關鍵詞: | 電化學傳感器 、紙基裝置 、微流體技術 、白蛋白 |
| 外文關鍵詞: | Electrochemical, Paper-based, Microfluidic, Albumin |
| 相關次數: | 點閱:88 下載:0 |
| 分享至: |
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尿液白蛋白對於腎臟病及心血管疾病之早期檢查是極為重要的項目,尿液白蛋白的自我評估檢測可以使病患在早期出現病徵時就能夠及早發現,同時能及早治療或控制病情,防範疾病於未然才是生醫檢測的未來趨勢。
而紙基微流體分析技術具有便利、快速、成本效益、不需要大型設備和專業操作人員等等的優勢而難以取代。同時也僅僅只需要微量的樣品和反應試劑,即可提供快速、高靈敏度的檢測,具有相當龐大的市場潛力,使得人們生活更加豐富及便利。
本研究介紹了定量微量蛋白尿的紙基碳糊電極電化學生物傳感器和採用絲網印刷電極的測量方法。紙基碳糊電極檢測方法主要是利用電活性物質莧菜紅與白蛋白之間的相互作用,結合成大分子的電惰性聚合物,使莧菜紅濃度下降其響應電流也會下降;而絲網印刷電極的測量方法則是利用施加電位使莧菜紅吸附在電極表面,而後與白蛋白之相互作用產生電惰性分子,在電極表面電形成惰層,導致電子轉移阻抗增加,產生響應電流下降的現象。
接著利用伏安法探討不同白蛋白濃度導致響應電流以及峰電位之變化,以小型電化學裝置進行微量白蛋白尿之定量分析。
Microalbuminuria is an extremely important item for the early examination of kidney disease. The rapid detection of microalbuminuria allows patients to aware of symptoms early and treat or control the disease. In order to prevent the disease from worsening, it must be the trend of biomedical detection in future. This study attempts to combine microfluidics convenient, fast, high sensitivity characteristics and advantages of low cost and so on electrochemistry, experimental design integration. Research and use the interaction between amaranth and albumin, which is an electroactive substance, to combine the properties of electrically inert polymers into macromolecules, conduct experimental discussions and design detection methods, and conduct paper-based carbon paste electrodes and screen-printed electrodes. The discussion, the use of electrochemical methods for experimental analysis. This research is designed through the idea of electrochemistry combined with rapid screening, and by comparing real sample analysis with hospital results, it is determined whether the experiment can be analyzed in real samples and with accuracy. The resulting recovery rate is about "100±10%", which is expected to be further researched and applied in the future.
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