由於廣泛的應用，奈米銀逐漸增加其暴露生物組織及環境的可能，其綜合的生物及毒理資訊仍舊缺乏，與人群健康及環境衛生的暴露及風險資訊仍未被系統性探索。本研究使用野生型斑馬魚胚胎動物模式建立毒性測試平台，藉以評估不同粒徑大小及不同表面修飾之奈米銀微粒毒性，探討合適毒性評估指標。亦探討暴露奈米銀微粒是否會對基因轉殖斑馬魚Trangenic line huORFZ 胚胎產生 ER Stress 反應，藉以評估其應用在毒性測試之可行性。利用模式生物斑馬魚胚胎建立毒性測試準則，胚胎暴露奈米銀微粒之毒性試驗，胚胎存活率會隨暴露濃度及暴露時間增加而遞減。斑馬魚胚胎暴露奈米銀微粒造成之畸形現象為：體型縮小；脊椎彎曲；心臟水腫；尾部彎曲。48 hpf 時，各組別之孵化率在高濃度組別與控制組有顯著差異。使用 Acute-to-Chronic Estimation (ACE) 軟體預測長期無效應濃 No-Effect Concentrations (NOEC) 之結果，四種奈米銀之長期 NOEC 預測值範圍約在 µg/L 範圍。毒性指標之實驗結果將有助於奈米材料危害風險評估。Homozygous huORFZ 胚胎暴露奈米銀微粒 SCS 實驗。初步發現暴露時間增加，更低濃度即會引起胚胎 ER Stress 表現。大致上胚胎 ER Stress 表現率會隨暴露時間增加而增強。而表現 ER Stress 之部位亦有隨時間進程而增加，依序為體表皮膚，感官嗅器，腦部與脊髓。huORFZ 胚胎能夠動態反映生理壓力，適合做為奈米材料毒性評估生物模式。

Silver nanoparticles (AgNPs) are widely used in a growing number of applications. However, with the accelerating production of AgNPs into commercial products, which raises health and environmental concerns. Potential toxicological implications are not sufficiently known. The objectives of the present study were to establish a toxicity screening platform with zebrafish model, to test the hypothesis that transgenic line huORFZ generate ER Strees after exposed to AgNPs. Adverse effects of AgNPs on hatching and development of zebrafish embryos are in line with previous reports. Among the methods, LC50 values ranged from 0.13 to 2.24 µg/ml. Chronic NOEC value estimated by ACE software with MPA model ranging from 0.00001 to 0.00014 µg/ml. These data provides valuable information for nanamaterial risk assessment. When homozygous huORFZ embryos exposed to AgNP (SCS), expression of ER Stress in GFP signals occured in skin, olfactory bulb, brain and spinal cord. The percentage of GFP signals and severity increase along with time.

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