文獻中提及蚊子之生物幫浦系統有三個致動器官分別為Oral cavity pump(CP)、Check valve(C-P Valve)及Pharynx pump(PP)，CP及PP作為傳輸流體之幫浦使用，C-P Valve則是能有效阻止流體回流，使流體具有單一方向之淨流量。本研究以蚊子之生物幫浦系統為範本進行仿生微幫浦之數值模擬與實驗，首先在二維模擬中比較有無C-P Valve兩種模型之流場情況，藉由改變CP及PP之作動方式及時間參數(α、β、T)設定後，使無致動閥門微幫浦體積流率(Q)表現與有致動閥門時相同，證實此改變下確實能取代中間閥門功能，接著依據實際晶片進行全尺寸之三維模擬，進一步觀察微幫浦內部流體流動情形。最後以聚二甲基矽氧烷(PDMS: polydimethylsiloxan)作為材料製作微幫浦系統實驗，並以正/負壓氣源作為兩薄膜之驅動力，使薄膜對流道內之流體具有擠壓及吸取兩作動，在量測實驗之體積流率(Q)後分析其與各參數之關係。

According to the literatures, mosquitoes use Oral cavity pump (CP), Check valve (C-P Valve) and Pharynx pump (PP) for their blood-sucking. CP and PP play the role of transporting fluid, and C-P Valve prevents backflow of fluid. In this research, pumping mechanism of mosquito's biological pump is studied by using both numerical simulation and experimental approaches and used as a model for current micropump design, At first, 2D numerical simulation of a mosquito's biological pump is used to investigated flow field, as well as net flow rate. By adjusting the actuation parameters (α、β、T) of CP and PP, one can achieve the same pumping performance that flow rate of the micropump without C-P Valve has the same flow rate as the micropump with C-P Valve. Therefore, C-P Valve within mosquito pumps can be removed in the current biomimetic micropump design. Secondly, according the sizes of biomimetic micropump design, 3D simulation model is built to study its pumping performance. Finally biomimetic micropump fabricated by PDMS (polydimethylsiloxan) is driven with both positive and negative air pressure. Two PDMS membranes with actuation mechanism like mosquito's biological pump can draw and squeeze the fluid in a micro-channel. The pumping performance is evaluated by adjusting actuation parameters including frequency, phase shift, ratio of volume, height of channel, width of channel between two membranes and duty ratio. The experimental results found that we can control the flow rate range from 0 to 60l/min or from -35l/min to -240l/min by adjusting frequency.

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