目的：本研究探討雙磷酸鹽及耐力運動介入對切除卵巢大鼠之骨代謝、能量代謝、骨組織型態與骨組織生物力學特性之影響。方法：以週齡35週之雌性Sprague Dawley (SD) 大鼠為研究對象，依照卵巢切除手術 (ovariectomy, OVX) 或偽手術 (sham surgery, SHA)、注射兩種劑量 (4 & 20, μg/kg) 之雙磷酸鹽 (藥名：zoledronic acid, ZA) 及耐力運動 (endurance exercise, EXE) 等實驗設計分做七組，分別為Sham (n=9)、OVX (n=8)、OVX+EXE (n=8)、OVX+ZA4 (n=9)、OVX+ZA4 +EXE (n=9)、OVX+ZA20 (n=9)、OVX+ZA20+EXE (n=9)。12週實驗介入後，動物安樂死所得骨骼樣本進行骨密度學、動態與靜態組織型態學分析，及以三點彎曲方法測量股骨之各項骨骼生物力學特性；血液樣本則進行各項骨代謝指標分析。統計方法以雙因子變異數分析進行去卵巢組別之各變項組間差異性比較，以獨立樣本t檢定進行SHA組與OVX組之差異性比較及皮爾森相關對於骨代謝血液指標與血糖調節指標相關性分析，當p＜.05時，視為達統計上之顯著水準。結果：經過12週實驗介入，OVX大鼠在接受耐力運動後，體重、三酸甘油脂與瘦體素濃度皆顯著下降，而且全股骨BMD、全股骨BMC與海綿骨BMD顯著大於無運動的OVX大鼠。另外，OVX大鼠注射ZA後降低瘦體素、骨鈣素濃度及次海綿骨區內之皮質骨區的礦化程度，但在海綿骨量與骨骼生物力學強度上皆顯著提升，而且全股骨BMC亦有顯著提升，其中注射ZA4的OVX大鼠在全股骨、股骨皮質骨及股骨海綿骨的BMD皆顯著大於注射ZA20的OVX大鼠。結論：骨頭透過ZA4注射後，不僅防止骨質大量流失而且骨頭負荷的表現亦有顯著提升。若再合併具有促進骨代謝效率之EXE，不僅促使各種骨骼組織之測試皆能有效改善去卵巢手術的影響，亦能降低體重與三酸甘油脂。

Purpose: The purpose of this study is to investigate the effects of bisphosphonate and endurance exercise (EXE) on bone metabolism, histomorphometry and biomechanical properties in ovariectomized rats. Methods: Thirty-five-week-old Sprague Dawley (SD) rats were used for the current study. All animals were assigned to seven groups in accordance with ovariectomy (OVX)/sham surgery (SHA)、zoledronic acid (ZA) injection at low or medium doses (4 & 20, μg/kg) and endurance exercise (EXE), which were Sham (n=9), OVX (n=8) , OVX+EXE (n=8), OVX+ZA4 (n=9) , OVX+ZA4 +EXE (n=9) , OVX+ZA20 (n=9) and OVX+ZA20+EXE (n=9). After 12-week interventions, all animals were euthanized. Samples of serum and bone were collected for analyses including serum marker assays, densitometry, static histomorphometry, dynamic histomorphometry and biomechanical three-point bending testing. Two-way ANOVA was used for data processing among ovariectomized groups. T-test was used for comparing the differences between the SHA group and the OVX group. Pearson’s correlation was used for analysising the relationship between serum of bone metabolism and glucose metabolism. Results: EXE rats were lower in weight, triglyceride, leptin and BMD of total femur, cortical bone and second spongiosa as comparing to non-EXE rats. On the other hand, ZA rats were lower in leptin, osteocalcin, and the mineralization degrees of second spongiosa and endocrinal bone comparing to non-injection rats. And, ZA rats were significantly higher in spongy bone volume, bone mechanical strength and total femoral BMD. Interestingly, ZA4 rats were higher in total, cortical and spongy BMD of femur when comparing to ZA20 rats. Conclusion: OVX rats treated by ZA4 had less bone loss and increased bone strength. Rats treated by ZA combined with EXE not only showed increased bone tissue but also decreased body weight and triglyceride.

林欣仕 (2008) 短期落地訓練對成長中母鼠的局部骨骼代謝之影響 (未出版碩士論文) 。國立成功大學，台南市。
蘇亦秀 (2013) 甲硫胺酸限制飲食與耐力運動對去卵巢大鼠骨骼之代謝, 組織型態與生物力學特性的影響 (未出版碩士論文) 。國立成功大學，台南市。
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