當兩個振盪頻率相近的系統結合，交換能量的過程中能保留多過於失去的能量，此現象稱之為強耦合。在此領域研究的推進下，已經克服低溫與真空的環境要求，實現常溫常壓下光與物質的強耦合，也已經將體積縮小到奈米級，這樣的系統給光學量子元件的相關研究提供一個基礎，對於科技量子化的未來，具有極高的價值與龐大的潛力。先前本實驗室已經開發了一個具有上述優良特性的強耦合系統，透過修飾有硫醇鍵與Cy5螢光分子的單鏈DNA，將奈米金粒子吸附到金膜上，形成一個約1 nm的間隙作為共振腔，並使Cy5螢光分子伴隨DNA嵌入共振腔中，當光被侷限在共振腔中，引發光與金屬間的電漿共振，並與螢光分子耦合，就會引發強耦合現象，出現全新的模態。
本研究選用連續胸腺嘧啶序列的單鏈DNA，利用紫外光誘發胸腺嘧啶形成二聚體，並開發在固體乾燥環境及溶液中的表面增強拉曼散射方法來讀取DNA本身微小的拉曼訊號，希望藉此驗證二聚體的產生。固體乾燥環境中的方法直接在石英片上滴製並乾燥金粒子與DNA，利用聚成帶狀分布的粒子群做為SERS基板來量測DNA，或是利用不同的預先吸附方式將DNA預先吸附在金粒子上，再將粒子撒在金膜上，使DNA被夾在粒子與金膜之間達成SERS條件，這些方法有著方便快速且節省材料成本的優點。溶液環境中的方法使用碘化鉀清潔金粒子表面的檸檬酸，混合金粒子與DNA後，加入硫酸鎂使粒子聚集，並將DNA夾在粒子間隙之間，達成SERS條件，這種方法能減少檸檬酸的拉曼訊號干擾，使DNA訊號更明顯。透過這兩種方法確定誘導二聚體方法的可行性後，未來就有機會將方法套用到強耦合系統中的DNA，二聚體的形成有可能間接牽動Cy5螢光分子，使其發生位移或轉動的結構改變，進一步將其中偶極矩的方向，調整至與腔內電場方向一致，進而提高耦合共振腔的產率及強度。

This work uses UVC to induce poly-thymine ssDNA dimerization and develops several surface-enhanced Raman scattering methods for validation. One employs naturally diffused SERS substrates to measure dry DNA samples, and the other method involves pre-adsorption of gold nanoparticles and DNA in a low pH environment, as well as utilizing the electrostatic properties of PAH to coat PAH and DNA onto gold nanoparticles. Both methods achieve SERS structures on gold films. The final method uses gold nanoparticles whose citrate caps have been removed, to sandwich the DNA strands in aqueous solutions. This method possesses high stability, enabling clear observation of spectral changes after thymine dimerization induction. A crucial element in our lab's strong coupling system involves fluorescent molecules embedded in ssDNA. By altering ssDNA conformation with dimerization, it is possible to finely tune the dipole moment orientation of fluorescent molecules, which can enhance its coupling strength to a nanocavity.

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