本研究為一自動化之整合型微流體晶片系統平台，藉由磁珠上具有與C-反應蛋白(C-reactive protein, CRP)高親和力以及高特異性的單股去氧核醣核酸適合體(CRP ssDNA aptamers)，進行磁珠適合體連結免疫分析法檢測流程，藉以偵測C-反應蛋白濃度的冷光值。CRP的濃度為人體發炎反應的指標，文獻中顯示CRP在低濃度時亦與心血管疾病的發生率呈現正相關，因此高敏感CPR(High-sensitivity CRP, Hs-CRP)可視為心血管疾病之風險評估因子。
利用微流體晶片系統平台之優點，如體積小、低耗能、反應快及靈敏度高等，可將傳統磁珠酵素連結免疫分析法縮小化於一晶片上。此晶片中包含微型幫浦、微型閥門及微型漩渦式混合器等元件，文中針對各元件作介紹與分析。不僅將微幫浦與微被動式閥門作一整合成具有新式微蠕動幫浦，提升了原先蠕動式幫浦的效率約4倍，背壓在微幫浦輸入頻率20 psi、90 Hz的情況下高達了85 cm-H2O。而微型漩渦式混合器在7 Hz、氣壓20 psi， 1.5秒內混合指數可由17.08% 增加到96.74%，使檢體能在最短的時間內有最佳的混合效率並且有效的提升檢測的靈敏度。接著將所有元件進行整合達到自動化傳輸流體、混合的功能，最後將微流體系統晶片應用於磁珠適合體連結免疫分析法C-反應蛋白之定量分析。
本研究成功的使偵測極限由傳統手動的0.125 mg/L，提高到0.0125 mg/L，並且檢體量減少為傳統檢測的一半，反應時間也由2小時30分縮短至25分，線性度也優於傳統檢測分析，並且透過外部控制系統可以同時平行處理四組磁珠適合體連結免疫分析法的C-反應蛋白之定量分析。證明了利用此C-反應蛋白檢測晶片在生醫檢測分析上確實大大地提升檢測的效能與靈敏度。

This study presents an integrated microfluidic chip consisting of pneumatic micropumps, normally-close microvalves and vortex-type micromixers for C-reactive protein (CRP) detection. CRP is a protein produced during inflammatory process. It was reported that serum CRP can be used for risk assessment of cardiovascular disease. In this study, CRP can be measured by using an immunoassay involving magnetic beads surface-modified with CRP-specific single-strain DNA (ssDNA) aptamers. The transportation of samples and reagents was performed by using the pneumatic micropumps and microvalves. The incubation and reaction processes were operated by using the vortex-type micromixer. The entire process can be then performed automatically on a single chip. The experimental results showed that the new pneumatic micropump could successfully increase the pumping rate about 4-fold higher than that without passive valves with the back pressure about 85 cm-H2O at the conditions of 20 psi and 90Hz. For the vortex-type micromixer, the mixing index could be increased from 17.08% to 96.74% within 1.5 seconds at the conditions of 20 psi and 7 Hz. The reaction time can be significantly reduced utilizing the proposed vortex-type micromixer. The functionality of the device has been demonstrated through its improved CRP detection limit from 0.125mg/L to 0.0125 mg/L when compared with traditional methods.

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