Tetra-methyl ammonium hydroxide ((CH3)4NOH, TMAH)被廣泛應用於半導體及光電產業製程中作為顯影劑， TMAH是高毒性、高氮污染物質，若無妥善處理就排出，則會造成嚴重的環境汙染。
本研究主要目的是想藉由應用多種分子生物技術，建立一個分析監測系統，評估現場高濃度TMAH廢水之厭氧生物處理系統中菌相結構的變化，即時監測現場處理系統菌群的消長，確實掌握水質處理成效。結果顯示，此分子生物技術分析監測系統確實具有良好的即時監測現場甲烷菌群的功能性，而且可以評估菌相是否有益於降解TMAH，未來甚至可以應用於不同目標菌群、不同目標化合物的分析。
本研究也以現場厭氧污泥為植種源進行批次實驗，並配合多種分子生物技術，探究具有直接降解TMAH能力之菌群。結果顯示，Methanomethylovorans spp.、Methanococcoide spp.和Methanosarcina spp.具有直接降解TMAH的潛力；實驗結果顯示，Methanosaeta spp.未參與TMAH降解過程。

Tetra-methyl ammonium hydroxide ((CH3)4NOH, TMAH) is widely applied as a developer in semiconductor and thin-film transistor liquid crystal display (TFT-LCD) industrial processes. This nitrogenous and highly toxical compound might cause serious environmental pollution if without a proper management.
There are two main parts in this research. First, build an analysis monitoring system by using multiple molecular biological techniques to investigate the change of microorganism population structure in anaerobic biological TMAH-containing wastewater treatment system. Additionally, monitor the growth and death of microorganisms in full-scale treatment system immediately and control the efficiency of wastewater treatment. The results showed that the analysis monitoring system could record the function of methanogens and evaluate the microorganism distribution have benefit for TMAH degradation or not. This system might be applied for the analysis of different microorganisms and compounds in the future.
Second, conduct batch experiment with anaerobic sludge from full-scale and apply multiple molecular biological techniques to find out those microorganisms which could degrade TMAH directly. From batches, Methanomethylovorans spp., Methanococcoide spp. and Methanosarcina spp. might have the potential candidates of TMAH degraders, and Methanosaeta spp. was presumably not related to TMAH degradation.

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