近年來光介電泳平台(ODEP)廣泛的應用在微流體系統內進行微粒子和細胞之操控和分離。當以投影機光源投射在光導材料上時，此時因為電子施體(donor)-受體(acceptor)間之接觸面積較大，材料吸收光能轉換為激發子(exciton)後，能有效被分離(dissociation)成電子與電洞，在主動層中的電子與電洞將引發不均勻電場來對介電物質產生一排斥或吸引的力。藉由移動或改變光的圖案可以對細胞或微粒子進行操控。光介電泳平台在微流體相關應用是一個強大的工具，但卻很少有藉由理論分析模型來得到正確的光介電泳之力量大小。傳統的介電泳模型(DEP)無法套用在光介電泳平台(ODEP)。
光介電泳平台有許多會影響力量大小的變數例如頻率、電壓大小的改變或者液體的種類等。對於開發一個可以準確預測光電泳力量大小的模型是重要且必須的，使得可以在實驗前就知道不同情況下力量大小的比較，以達到光介電泳平台的最佳化設計和提高其他相關應用的可行性。本研究提出一個嶄新的－“電壓轉換效率”模型計算跨在操控微粒子的液體層之有效的電壓來提高光介電泳力的數值分析的準確性。並採用高斯分布之邊界條件來設定光照射區域之虛擬電極分布有效區別傳統介電泳電極的邊界條件。在兩個已發表的操控結果－聚苯乙烯珠和本文的乳化液滴實驗，我們的模擬結果與實驗值合理的吻合，其中最大誤差範圍在低於百分之六點二五以下，這是傳統模擬光介電泳模型所難以達到的。未來此模型也可以應用在藉由增加光的強度和微調光波長或使用其他光導材料(例如高分子聚合物)或最佳化光導材料的厚度來計算增加電場大小和力量大小。“電壓轉換效率＂模型對於未來光介電泳平台在微流體、微機電等相關應用提供一個可以用來預測光所導引出的電場分布及光介電泳力大小的可靠分析工具。
本研究也成功地以光介電泳力來實現對水包油乳化液滴的操控和分離。藉由使用改變光不同的掃描速度，水包油乳化液滴能被高解析度的分離並且進行單顆的乳化液滴的操控，有別於傳統分離和操控乳化液滴技術。首先，不同大小的乳化液滴(40-43, 20-30, and 2-5微米)可以先成功地被粗分成三等份。以漸進梯度改變光的移動速度成功細分大小差異2.5微米的乳化液滴。為了避免液滴的碰撞和重疊現象影響分離效果，本研究提出一個新的設計－“光軌＂來分配不同大小的乳化液滴在各自的光定義之移動軌道中來進行分離。根據此方式五種不同大小的乳化液滴 (30, 20, 10, 7.5, and 5微米)成功的分離開。最後，本研究也利用光介電泳平台針對在微流體乳化晶片中產生乳化液滴時伴隨著衛星液滴的困擾，成功地結合乳化晶片並將衛星液滴和主要液滴做有效的分離。此發展平台對於控制且提升高品質的乳化液滴有著很高的潛力，並且可以應用在許多乳化技術的產業例如化妝品工業、食物配製、藥物應用，與去氧核醣核酸膠囊、微米粒子的藥物傳遞系統、單分子酵素分析等相關應用。

ODEP platform is a powerful light-based technique for microfluidic application. When illuminated by a projected light beam, the photo-induced charge carriers created by the electron transfer of excitons at a donor/acceptor interface in the photoconductive layer, disturbs the uniformly-distributed electric field applied on the ODEP devices. However, only a few attempts to characterize the induced ODEP forces yielded satisfactory accuracy. It is imperative to develop an effective model which can accurately simulate the ODEP force with consideration of the underlying parameters, like changes in frequency, voltage, medium, etc., which can significantly affect the capability of other applications in the ODEP platform. This study reports on the results from numerical simulation of the ODEP platform using a new model based on a voltage transformation ratio (VTR), which takes the effective electrical voltage into consideration and the ODEP force can be numerically calculated with a reasonable accuracy. Gaussian distribution of the light patterns was adopted to depict the light propagation on the ODEP electrode edge to distinguish with the conventional dielectrophoresis (DEP) electrodes. The ODEP forces for emulsion droplets and polystyrene beads were investigated. Results showed that the numerical simulation is in reasonably agreement with experimental data for the manipulation of polystyrene beads and emulsion droplets, with a variation less than 6.2%. The proposed model can be applied to simulations of the ODEP force and may provide a reliable tool for estimating induced dielectrophoretic forces and electric fields. In order to increase the electric field and the resulting ODEP force, one can increase the intensity of the light, fine-tune the wavelength of the light, use other photoconductive materials (such as polymers), or optimize the thickness of the photoconductive materials. The proposed VTR model provides a new approach to calculate the ODEP force to calibrate the voltage transformation ratio. This may provide researchers a useful tool for accurately predicting the force on beads, droplets or cells, which is crucial for microfluidic applications.
A microfluidic platform for dynamic manipulation and separation of oil-in-water emulsion droplets by using optically-induced dielectrophoresis (ODEP) is also reported in this study. By utilizing different scanning speeds of a moving light beam, the oil-in-water emulsion droplets can be moved and separated with a high separation resolution. A first demonstration of this platform is pre-separation and fine separation of emulsion droplets. Three groups of droplets with different sizes (40-43, 20-30, and 2-8 m) can be roughly separated first. The fine separation of emulsion droplets with a radius difference of 2.5 m can be performed using a moving light beam with a gradual gradient of moving speeds. To avoid the collision and overlapping of the droplets, a new separation scheme - “light-track" to assign individual moving track for each droplet was adopted by using well-defined moving light patterns. Accordingly, droplets with five different sizes (30, 20, 10, 7.5, and 5 μm) can be successfully separated. The second demonstration is to separate satellite and master emulsion droplets generated from microfluidic emulsion chips. The ODEP force was also characterized by balanced with drag force. It was found that conductivity of the medium play an important role on the ODEP force. The bigger conductivity is, the less ODEP force is. The developed ODEP platform may be promising for enhancing the quality of the emulsions, which can be applied a variety of fields such as cosmetics, food preparation, and medicine applications, encapsulation of DNA, nano-particles for drug delivery system, single-molecule enzyme analysis.

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