隨著醫療技術的快速發展，細胞治療正逐漸從學術研究轉而應用至臨床試驗及疾病的新興療法中，目前最熱門的領域為癌症治療與再生醫學。細胞治療的流程可大致分為三大步驟：獲得目標細胞來源、於體外進行放大培養，在達成目標細胞數量後移植進患者體內；其中，如何在有限的培養空間中以較高效率的方式將細胞進行放大培養，是完成細胞治療程序中值得優化且不可或缺的一環。
相較於常用的聚苯乙烯培養皿與培養瓶，由塑膠薄膜製成的軟式細胞培養袋更能靈活應用培養箱的空間，可滿足各種細胞的大規模培養需求。環境中的氧氣與二氧化碳可直接透過袋體材料進行交換，培養基的更換與細胞收集則透過袋體所連接的管路進行，培養環境更為封閉，可降低因人為操作或環境因素而造成的汙染。使用低水氣通透率的袋體材料，可避免培養基因蒸發造成的濃度改變。然而，培養基中添加的動物血清及細胞生長過程中產生的蛋白質，可能吸附在液體與袋體接觸的表面，同樣會使培養基營養物濃度改變，甚至因細胞殘留在袋體中而使細胞收穫效率低下，因此選用抗生物分子貼附的材料作為細胞培養袋亦是一個可優化的特性。
本研究與台塑集團合作，使用摻有不同濃度雙離子的乙烯-醋酸乙烯酯薄膜材料，旨在驗證其材料強度、氣體通透度、生物相容性及低生物分子殘留等特性，選定最適合作為細胞培養袋的候選材料。所有摻有雙離子的乙烯-醋酸乙烯酯薄膜皆展現高於國際規範的70%細胞存活率，且細胞凋亡比例皆小於15%； ZW20也於細胞貼附與蛋白質殘留實驗中，具備最少的細胞貼附密度2.12×103 cells/cm2及最少的蛋白質殘留濃度1.53 μg/mL，其中蛋白質殘留濃度更顯著低於市售對照品SAINT的3.82 μg/mL，展現其作為細胞培養袋基材的開發潛力。為將培養袋的適用性衍伸到錨固型細胞，亦著手研發溫感型細胞載體，以提供錨固型細胞在懸浮環境中的生長平台。

With the rapid development of medical technology, cell therapy is gradually shifting from academic research to clinical trials and emerging treatments for diseases. The cell therapy process can be divided into three major steps: obtaining the target cell source, amplifying and culturing the cells in vitro, and transplanting the cells into the patient's body once the required number of cells has been reached. Among these steps, amplifying and culturing the cells more efficiently in a limited culture space is a worthwhile improvement and an essential part of completing the cell therapy process.
Compared to the commonly used polystyrene Petri dishes and flasks, cell culture bags made of plastic film are more flexible in applying the space in the incubator. Commercially available cell culture bags with culture volumes ranging from a few milliliters to a few liters can satisfy the needs of large-scale culture of various types of cells. Oxygen and carbon dioxide can be exchanged directly through the bag material. Medium changes and cell collections occur through tubing connected to the bag. Thus, cell culture bags are more "closed" than traditional Petri dishes, reducing contamination caused by human operation or environmental factors. A closed cell culture bag also helps maintain a constant cell culture environment. Using a bag material with low water vapor permeability prevents changes in concentration caused by the evaporation of water from the culture medium and maintains environmental consistency for cell proliferation. However, proteins produced during cell growth and animal serum added to the culture medium may be adsorbed onto the surface of the bag in contact with the liquid. This changes the concentration of nutrients in the culture medium and can cause inefficient cell harvesting due to cell residue in the bag. Therefore, the use of anti-biomolecule adherence materials for cell culture bags is an ideal solution.
In this study, we cooperated with Formosa Plastics Corporation to use ethylene vinyl acetate (EVA) film doped with different concentrations of zwitterions, intending to verify their material strength, gas permeability, biocompatibility, and low biomolecule residue property, and to determine the most suitable material for the cell culture bag candidates. All the zwitterion-doped EVA films demonstrated a cell viability of 70% and an apoptosis rate of less than 15%. At the same time, ZW20 also showed the lowest cell attachment density of 2.12×103 cells/cm2 and the lowest protein residue concentration of 1.53 μg/mL in the cell attachment and protein residue assays. The protein residue concentration was significantly lower than that of SAINT (3.82 μg/mL), demonstrating its potential as a substrate for cell culture bags. In order to extend the applicability of the culture bag to anchorage-dependent cells, the thermoresponsive cell carrier was also developed to provide a platform for anchorage-dependent cells to grow in a suspended environment.

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