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研究生：	莊朝惠 Zhuang, Chao-Hui
論文名稱：	多孔性水膠薄膜的製備及仿淋巴液過濾應用研究 Fabrication of Porous Hydrogels and Their Applications on Artificial Lymphatic Drainage
指導教授：	劉俊彥 Liu, Chun-Yen
學位類別：	碩士 Master
系所名稱：	工學院 - 材料科學及工程學系 Department of Materials Science and Engineering
論文出版年：	2022
畢業學年度：	110
語文別：	英文
論文頁數：	74
中文關鍵詞：	淋巴引流、水膠、雙電性高分子、過濾膜、抗細胞貼附
外文關鍵詞：	lymphatic drainage, hydrogels, polyampholytes, filtration film, cell anti-adhesion
相關次數：	點閱：65 下載：0
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本研究合成了一系列多孔性水膠，並製備了仿淋巴液過濾裝置。使用自由基聚合方法，合成了含有正電單體及負電單體的多孔性水膠。透過電子顯微鏡分析結果，所合成出的多孔性水膠之平均孔洞大小為10μm。加入丙烯醯胺(Acrylamide)降低了平均孔洞大小且降低了水膠的機械性質。此外，增加雙電性高分子的含量會大幅地降低平均孔洞大小，且含有較多雙電性高分子的水膠展現出較好的抵抗外力的能力。為了研究水膠的抗細胞貼附特性，將水膠用於人類真皮纖維細胞(NHDF)的培養。結果顯示，水膠具有很好的抗細胞貼附特性，原因為水膠整體的帶電性為微負電性。在螢光顯微鏡的觀察下，PA151水膠表現出最好的抗貼附特性。另外，水膠前驅液用於濾紙表面的塗布，改質後的濾紙表現出極佳的仿淋巴液過濾效果，仿淋巴液中的細胞皆可被擋住，不通過濾紙。由上述的實驗結果可得知，水膠前驅液及改質過的濾紙可被實際應用於淋巴液的引流。此研究中所使用的是具新穎性且簡單的製備方法，透過此方法製備的水膠過濾膜可以成功達到淋巴過濾的目的。

In this study, we demonstrate the synthesis of porous hydrogels and the fabrication of filters for artificial lymph drainage. A series of porous hydrogels with positive and negative charged pendant groups was synthesized via free-radical copolymerization. Based on the SEM analysis, the synthesized average pore size is about 10 μm. The incorporation of acrylamide (AM) increases the average pore size and decreases the mechanical strength due to the decrease of ionic pendant group interactions. Furthermore, increase of polyampholyte content decreases the average pore size obviously. Hydrogels with more polyampholyte (PA) contents demonstrate the better resistance of external force. To study the anti-adhesion of the synthesized hydrogels, normal human dermal fibroblast (NHDF) cells were seeded on to the porous hydrogels. Due to the predesigned negative charge, the synthesized hydrogels show significant anti-adhesion of cells. Based on the fluorescence imaging results, the synthesized PA151 hydrogel shows the best cell anti-adhesion. Moreover, the synthesized hydrogels were used to modify the surface of paper filters showing excellent artificial lymph fluid filtration. The fluorescence images of the filtrates via the synthesized surface modified filters show that NHDF cells were successfully filtered out. Based on the results, the predesigned hydrogel precursors and the surface modified paper filters are available for the lymphatic drainage. The fabrication method created in this study is a novel idea and it is an easy way for the preparation of lymph fluid filtration equipment.

Abstract I
中文摘要 III
誌謝 IV
Contents V
List of Figures VIII
List of Tables XIII
1.	Introduction 1
1-1	Preface 1
1-2	Research motivation 2
2.	Literature Review 4
2-1 Lymph circulation 4
2-2 Introduction of Lymphedema 6
2-2-1 Treatments of Lymphedema 8
2-3 Introduction of Hydrogels 12
2-4 Characteristics of Hydrogels 13
2-4-1 Swelling ratio 14
2-4-2 Porosity 15
2-4-3 Mechanical Properties 17
2-5 Supramolecular Hydrogels 19
2-6 Characteristics of Poly(HEMA) 23
3.	Experimental Section 24
3-1 Materials 24
3-2 Instruments 25
3-3 Experimental Section 27
3-3-1 Synthesis of Polymerized Porous Hydrogel Film 27
3-3-2 Preparation of Porous Hydrogel Films 30
3-3-3 Solid State 13C-NMR (SSNMR) Analysis 32
3-3-4 Equilibrium Water Content Measurement 33
3-3-5 Surface Morphology Analysis 33
3-3-6 Compression Test of Polymerized Porous Hydrogel Membrane	33
3-3-7 Rheological Measurement of Polymerized Porous Hydrogels	34
3-3-8 Cell Anti-Adhesion Test of Porous Hydrogels 35
3-3-9 Thermal Properties of Polymerized Porous Hydrogel Membrane 35
3-3-10 Preparation of Artificial Lymph 36
3-3-11 Construction of Lymph Filtration Device 37
4.	Results and Discussion 39
4-1 Characterization of Porous Hydrogel Film 39
4-1-1 Solid-State 13C-NMR Characterization 39
4-1-2 Effect of Crosslinking Degree on Porous Hydrogels 43
4-1-3 Effect of Ionic Monomer Contents on Porous Hydrogels 43
4-1-4 Effect of Acrylamide Concentration 46
4-1-5 Effect of Polyampholyte on Hydrogels 51
4-1-6 Cell Adhesion Analysis Stained by CFSE 58
4-1-7 Fabrication of Filtration Device via Hydrogel Modified Filter 60
4-1-8 Analysis of Hydrogel Modified Paper Filter 63
4-1-9 Filtration Test Using the Fabricated Hydrogel Modified Filter	66
5.	Conclusions 68
6.    Reference 70
                                    

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校內：2025-08-31公開
校外：2025-08-31公開

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