Peptide nucleic acid ( PNA )是一個模仿DNA/RNA 的類似物。 PNA是由Peter Nielson所發表的概念，他設計的PNA 分子是以psuedo-peptide 的骨架，取代原本 DNA 的 ribose-phosphate 骨架。一種能與 DNA 或 RNA產生氫鍵，且其主幹並非五碳醣及磷酸所組成的結構。PNA 分子沒有磷酸根的存在，因此結構不帶電荷，不會有電荷排斥的現象，因此預期 PNA 與 DNA 會有好的親合力。 PNA 具有良好的熱穩定性，且對於蛋白酶及核酸酶也有很好的抵抗力，因此應用antigene 與 antisense 上，範圍很廣。

我們設計一個新的PNA分子結構，由原本的PNA改良而來，藉由我們的合成策略，我們成功的合成新的PNA分子。將來，我們將改變鹼基或是替換鹼基為新合成出來的5-cyanouracil類似物，合成出新的PNA分子，試著將與DNA結合，與原本的PNA分子做比較，觀察新的PNA分子應用於生化上的生物活性如何 。

Peptide nucleic acid ( PNA ) is an analogue which imitate DNA/RNA. PNA is a concept which has been supply by Peter Nielsen, which PNA molecule he designed replace DNA ribose-phosphate backbone with psuedo-peptide backbone, it can be combine with DNA or RNA, and its backbone does not been compose by ribose-phosphate.Phosphate is not exist in the structure of PNA. since there are no charge in the structure of PNA, the binding between PNA and DNA is stronger then between DNA and DNA due to lack electrostatic repulsion. The high thermal stability and resistance protease and nuclease make PNA’s ideal reagent is an anti-gene and anti-sense study.

We design a new PNA structure form original PNA. We can synthesis the new PNA structure by our synthesis strategy. We will change organic base or replace organic base with 5-cyanouracil analogues to synthesis more new PNA structures like this new structure, we will combine the PNA with DNA and compare with the original PNA. We want to know the result than new PNA monomer applied on biochemistry.

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