為了解神經細胞的於二維平面活動的行為，必須將神經細胞培養在位於修飾過的基材表面上，由細胞外基質或細胞黏著因子所構成之幾何設計圖形中。最近的研究中由於微米壓印技術的使用，使得控制細胞的位置和生長得以實踐。我們的研究目的是設計出簡單不昂貴的顯微鏡微米壓印器來調控可引導細胞生長的圖形於基材表面上的位置。 於此研究中，利用以微米製程所製造出的聚二甲基矽氧火完 (PDMS) 的微米印磋，將右旋光性聚離胺 (PDL)， 細胞黏著子，轉印至經修飾過的基材表面上。而且，我們觀察PDL溶液濃度，即印墨濃度，與由壓力感測器所得之轉印壓力的搭配關係， 對染有螢光的多肽類所壓印而成之圖形的線寬所造成的影響。對不同印墨濃度範圍及施加壓力間所造成的結果是為更進一步的研究應用中所需要的，因此我們提供更進一步的觀察。我們相信我們的設計搭配更進一步的配備與修理飾能提供實用的的微米壓印器來幫助研究神經細胞的行為,如形成神經細胞構成的圖形及神經網路，且可避免於壓印時施加過大的壓力而造成印磋的變形，導致其印磋上的圖紋之身寬比降低而使得印墨較低的有效轉印或拓印等情形。然而轉印的結果亦和PDL的濃度有關。

　　To culture neuronal cells grown in a geometric pattern on a modified substrate plate is essential to understand the neuronal cell behavior. Recent studies have made it possible to control neuronal cell positioning as well as outgrowth by using the microcontact printing technique. The aim of this study was to design an inexpensive microscopy-based microcontact printing device for aligning the pattern to the substrate for guiding neuronal growth. In the study, the microfabrication of the microcontact printing stamp of Polydimethylsiloxane (PDMS) was used to define the cytophilic region by transferring poly-D-lysine (PDL), cell adhesion factor, to surface-modified substrate. The cytophobic region was then developed by immersing the printed PDL substrate in bovine serum albumin (BSA). Our study examined the concentrations of PDL to form cytophilic region and provides a micro contacting scheme to produce a reliable structural pattern on the substrate. Furthermore, we investigate the relationships between the ink concentration and printing force monitored from torque sensor to the peptide pattern from fluorescent image of printing lines. Further studies are needed to systematically investigate the suitable ranges for varied ink concentrations. It is believed that our device with some further modification can provide an inexpensive microcontact device to study neuronal behaviors, i.e. neuronal patterning and network/synapse formation. Too low in applying force might cause the poor transfer rate but the too high in applying force might cause the deformation of stamp due to the low ratio of depth to width of the stamp. However, this result is affected by the PDL concentration applied.

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