1992年白點症於中國首次爆發，作用機制至今尚未被完全瞭解，因此解決此疾病為日前重要的議題。藉由先前實驗室的研究，我們發現白點症病毒在感染後的蝦血細胞中，所出現的有氧糖解和脂質代謝現象可有利病毒複製，然而糖解酵素和其他下游路徑的改變仍未被探討。本研究目的是由分子層面探討白點症病毒感染期間，參與有氧糖解的酵素群對病毒複製之影響，本研究著重酵素群HK、PFK、PK 和LDH在病毒感染前後的活性及基因表現量的變化。結果顯示在病毒感染後十二小時，這些酵素之基因表現量皆無顯著差異;而酵素活性除了HK外，其他關鍵酵素於蝦血細胞内皆有所上升；但此現象在感染二十四小時後則消失。在HK、PFK和LDH基因默化，或是葡萄糖類似物的處理下，可導致病毒之基因體和基因表現量降低。先前實驗室研究也證實病毒感染晚期的脂質代謝對於病毒複製十分重要，且糖解作用後的Acetyl-CoA是脂質代謝的重要原料，它能夠透過ACC的作用以利脂肪酸的生合成。本研究卻發現在ACC抑制劑或基因默化干擾下，發現即使細胞内之病毒基因體數降低，但整體病毒釋放量有著非預期的上升現象。由此可知，參與有氧糖解酵素群對於在病毒複製具有重要性，但ACC 在其中所扮演之角色仍有待釐清。

White Spot Syndrome has become an important issue to counter in aquaculture since the first outbreak of it in China in 1992. White Spot Syndrome Virus (WSSV) was previously discovered to induce aerobic glycolysis, also named Warburg effect, and lipid metabolism in WSSV-infected hemocytes. However, the change of aerobic glycolytic enzymes and downstream factors during WSSV infection is still unclear. The objective of this study is to examine the behavior of glycolytic enzyme during WSSV infection at a molecular level. Four influential glycolytic enzymes, Hexokinase (HK), Phosphofructokinase (PFK), Pyruvate kinase (PK) and Lactate dehydrogenase (LDH), were selected and subjected to the mRNA and activity analysis. Results showed that mRNA expression of these enzymes was not significantly induced at 12 hpi. These enzymes showed a high enzyme activity in hemocytes at 12 hpi except HK. At 24 hpi , the upregulation of four glycolytic enzymes was no longer observed. Silencing of HK, PFK and LDH and glycolysis interruption by 2-DG demonstrate a lower viral gene expression and virus genomic copies. On the other hand, Lipid metabolism is important for the virus replication and it is also one of the downstream effects of aerobic glycolysis as Acetyl-CoA is a key metabolite linking glycolysis to lipogenesis. The Silencing or inhibiting ACC revealed that virus release per cell is increased, even though the virus genome copies is low compared to other control group. In conclusion, WSSV-induced aerobic glycolysis plays an important role in the WSSV replication, but the role of ACC in WSSV infection require further clarification.

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