微生物製劑目前大多使用植物生長促進根系細菌與促進植物生長之真菌進行開發，然而內生菌為植物生長發育中重要的參與者，其中種子內生菌參與了種子的初期萌發與微生物族群的建立，並可以藉由母株垂直傳播至子代，具有微生物製劑開發的潛力。本篇論文研究分離高氏柴胡與兔尾草之種子內生菌，探討其促進植物生長細菌 (PGPB) 的能力，並評估其作為微生物製劑的可能性。結果顯示多數菌株皆有生產IAA的能力，且有51.6%的菌株擁有溶磷能力。對峙結果顯示分離自高氏柴胡的假單胞菌屬BKEP3_20可以抑制5個屬7種土傳性病害，分離自兔尾草的泛菌屬UCEP4_2則專一抑制白絹病。經由VOCs的測試發現BKEP3_20與UCEP4_2可以釋放揮發性有機物顯著抑制對白絹病真菌的生長。盆栽試驗使用胡蘿蔔來驗證促進植物生長能力，分別施用BKEP3_20、UCEP4_2與共接種來驗證。結果證實施用UCEP4_2可以顯著促進胡蘿蔔生長，擁有作為微生物肥料的能力；種子萌芽試驗證明了此兩株分離株於白絹病病土環境下，可以增加胡蘿蔔的發芽率。綜上所述，假單胞菌屬之BKEP3_20更適合開發作為廣效型生物防治劑，而泛菌屬的UCEP4_2除了適合做為微生物肥料使用之外，也適合開發為胡蘿蔔白絹病的生物防治劑。

Endophytic bacteria are crucial participants in plant growth and development, with seed endophytes playing a significant role in the initial germination of seeds and the establishment of microbial communities. These bacteria can be vertically transmitted from the parent plants to the offspring, indicating their potential for development as an microbial agent. This study focuses on isolating seed endophytes from Bupleurum kaoi and Uraria crinita, exploring their plant growth-promoting bacteria (PGPB) abilities, and evaluating their potential as microbial agent. The results show that most isolates are capable of producing indole-3-acetic acid (IAA), with 51.6% of the isolates possessing phosphate solubilization ability. Antagonistic tests revealed that Pseudomonas sp. BKEP3_20, isolated from B. kaoi , inhibited seven soil-borne pathogens across five genera, while Pantoea sp. UCEP4_2, isolated from U. crinita, specifically inhibited Sclerotium rolfsii. Volatile organic compounds (VOCs) tests showed that both BKEP3_20 and UCEP4_2 release VOCs that significantly inhibited the growth of S. rolfsii. Pot experiments using carrots demonstrated that UCEP4_2 significantly promoted plant growth, suggesting its potential as a biofertilizer. Seed germination tests indicated that both BKEP3_20 and UCEP4_2 increase carrot germination rates in soils infected with S. rolfsii. In conclusion, Pseudomonas sp. BKEP3_20 is suitable for development as a broad-spectrum biocontrol, while Pantoea sp. UCEP4_2 is appropriate not only as a biofertilizer but also as a biocontrol agent for carrot southern blight.

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