三維交錯微管道具有許多應用的可能，例如流體轉換、混合等。此論文主要目的為延續前人的研究，詳細探討管道之不同參數(參數包括角度、深寬比、l參數)對微混合器混合功能的影響，並找出最佳參數組合，使混合效能提高。
本研究利用CFD-RC 數值模擬軟體來確認此三維結構中的流場與混合特性，再以墨水、螢光染劑、螢光顆粒進行實驗，比對數值模擬結果與實驗結果是否有一致性。實驗的後處理方法是使用ImageJ 影像處理軟體對螢光顆粒計數及染料光強度的結果進行分析，得知混合效能的好壞。相信經過這些參數測試後，能對相關元件中的流體控制特性提供一些有用的參考。
結果顯示，角度90度、l參數為25 μm、深寬比第一到第四截面為0.4的混合器具備了分離合併（SAR）的多層板流與混沌的混合機制，展現了相當優異的混合效能，未來具有流體輸送、控制、混合應用的可能。

Three-dimensional crossing microchannel can be applied in many ways, such as liquid switching and mixing. To extend previous studies, this thesis investigates the effects of different parameters―angles, aspect ratio, and l parameter on the efficiency of micromixer. Attempts have been made to find out the optimized combination of parameters so as to enhance the mixing efficiency.
This thesis utilized CFD-RC simulation software to calculate the flow and mixing characteristics―followed by experiments using ink dye and fluorescent dye to examine the consistency between the results of the simulation and experiments. The post-processing software ImageJ was used to analyze particle counting and light intensity so as to quantify the mixing efficiency. This thesis aims to provide useful guidelines for the control of flow in microfluidic after the comprehensive investigation of these parameters.
As the results showed, microchannel with the crossing angle of 90 degrees, l parameter equal to 25 μm, and aspect ratio of 0.4 enhanced mixing efficiency significantly. In this regard, microchannel with 3-D crossing structure features a simple but robust mixing device.

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