本研究主要以發展糖化血紅素分子模版感測電極為主要目標，架構分子模版電極時，以定電流聚合導電性高分子模版，以砒咯單體為主，然後以鐵氰化鉀 (K3Fe(CN)6) 與亞鐵氰化鉀 (K4Fe(CN)6) 的氧化還原反應做為電流感測之指示劑，並配合循環伏安法來記錄氧化還原峰值。首先先研究聚砒咯在不同厚度(聚合時間)以及電流密度時，對鐵氰化鉀與亞鐵氰化鉀的氧化還原，發現在定電流超過 3µA 後，氧化還原峰值從17~19µA 掉至 4~6µA，而聚合時間在超過 180秒之後，加入糖化血紅素時，無法產生訊號。研究糖化血紅素是否會在聚砒咯膜上在 -0.2~0.6V vs. Ag/AgCl 之間是否會氧化還原反應，發現作了清洗前、後還有再吸附都發現無氧化還原。還有再吸附不同時間的氧化峰值約在1個小時後便達飽和。
再吸附不同濃度糖化血紅素發現在約 0~60 µg/ml 得到線性關係較佳。從飽和吸附曲線，我門按照 Scatchard plot 的公式

[B] 是吸附在分子模版上的目標物濃度， [F] 是在未鍵結餘分子模版的目標物濃度， Kd是解離平衡常數，N 是辨識性孔洞總量，因此做 [B]/[F] 對 [B] 的圖，線性迴歸後可得 Kd 以及 N ，在本實驗中我門得到 Kd= 1250 µg/ml，N=187.75 µg/ml。在選擇性方面我門做了血紅素與糖化血紅素，選擇比率約0.44，還有糖化血紅素對免疫球蛋白 ( IgG )、溶菌酶( Lysozyme )、人血清白蛋白 ( HSA )，選擇比率各約0.38、0.68、0.54。後面並附上一些分子模版表面觀察。並有與崑山科技大學電機系黃俊岳教授的碩士班張志弘同學合作，他製作 home-made potential state 接上糖化血紅素分子模版電極後來量測糖化血紅素，經修正後得到較好的圖。另還有與成功大學工程科學系李國賓教授的碩士班學生黃朝均同學合作微流體血糖感測器。

In this research, we develop Hemoglobin-A1c MIP sensor for purpose, to construct this MIP sensor, we choose Pyrrole as a monomer, generate a template by galvanostatic electropolymerization, then consider red prussiate of potash and yellow prussiate of Potash as indicator for sensing Hemoglobin-A1c, we record the redox current by Cyclic Voltammetry. We study first is that different film thickness versus redox current and different applied current for electropolymerization versus redox current, we find that we can’t sense redox current when electropolymerization over 180sec with HbA1c, and the applied current over 3µA, the redox current reduce from 17~19µA to 4~6µA . And we also study if we just use HbA1c on Polypyrrole film based on Pt electrode to scan CV how the redox current change, compare the before wash, after wash and rebinding HbA1c redox current, all redox current can’t be seen.
Study the linearity of different rebinding Hba1c concentration versus decrease current, we got better linearity between 0 to 60 µg/ml. And before we illustrate scathchard plot, need make saturated curve first then take the data to scatchard plot equation

There [B] is target concentration adsorbed on MIP surface, [F] is the target concentration of unbind HbA1c in solution, Kd is dissociation constant, N is selective porous on MIP surface, so we take [B]/[F] versus [B] to make a curve, after linear regression we can get the slope and intercept, Kd=1250µg/ml, N=187.75µg/ml. Also we research how’s the selectivity of Hb/HbA1c, IgG/HbA1c, Lysozyme/HbA1c, HAS/HbA1c , and we got 0.44, 0.38, 0.68 and 0.54 respectly.
In the last study, I have some data from cooperation with the E.E graduate student C. H Chang of Prof. C. Y. Huang from KunShan university, he develop a home-made potential state, and we compare the data with EG&G 263A potential state. The other hand we also cooperate with E. S. graduate student C. J. Huang of Prof. G. B. Lee from ChengKung university, we develop a microfluidic system for sensing glucose.

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