多年以來，吸煙所產生之二手菸危害廣為人知，二手菸中所含有微量之多環芳香烴（PAHs）與香菸中特有亞硝胺（TSNAs）等化合物均已被美國EPA標定為致癌物，在國外之文獻中，也清楚的提到二手菸中之微量物質會對人體產生可遺傳的細胞突變。而香菸所產生之煙霧有分兩種，主流菸（Mainstream smoke）與側流菸（Sidestream smoke），主流菸即由吸煙者經由濾嘴吸入之煙霧，而側流菸為菸頭燃燒而形成之煙霧，由於側流菸為不完全燃燒，故產生之毒性物質可為主流菸之數倍，而二手菸之組成即為80%的側流菸加上20%的主流菸，故本研究以香菸側流菸之致突變性來評估二手菸對人體造成的基因毒性。
　　許多學者指出，二手菸具有之致突變性物質幾乎都會附著於空氣的懸浮微粒之中進入人體的肺部而造成傷害。本研究為探討不同種類之菸品所產生之側流菸，故準備了八種不同濃淡口味、不同菸草配方之香菸與雪茄，點燃後以濾紙吸附住空氣中之懸浮微粒，以GC-MS分析其中之16種多環芳香烴（PAHs），並以元素分析儀得知菸葉本身之各種元素含量，最後以Ames測試之沙門桿菌變異種TA98與TA100測試其致突變性之強弱。
　　結果可知，香菸煙霧中之氣膠在Ames測試中均需以鼠肝酵素（S9）活化之後才會有致突變性的展現，故香菸煙霧中大多為間接致突變性物質，且PAHs 含量與煙草中之氮含量為對致突變性影響最大之因子。而雪茄產生之側流菸的基因毒性最強，而若焦油含量相同之紙捲菸，雖然以烤菸葉的英式捲菸PAHs之含量會略高於美式捲菸，但因美式混合型捲菸之氮含量較高，故基因毒性為美式捲菸較高。而焦油含量僅為濃菸之一半的淡菸，側流菸中之致突變性物質僅略低於濃菸。香菸中薄荷香料的添加則對致突變性無明顯的影響。

　During the past decade or so cigarette smoking had been proven to be hazardous to health. The chemical compounds that envrionmental tobacco smoke (ETS) contain, such as Polycyclic Aromatic Hydrocarbons (PAHs) and Tobacco specific N-Nitrosamines (TSNAs) all be marked as the potent carcinogenic compounds. Many refernces also indicate that environmental tobacco smoke contain trace component which will cause genetic mutation.
　ETS can divide into mainstream smoke (MS) and sidestream smoke (SS) .MS means the smoke which smoker draw in through the filter, SS means the smoke caused by burns of the top of cigarette. Because SS result from incomplete burning, the toxic compounds are times of MS. The form of ETS is about 80% SS and 20% MS, so the study of this research analyse mutagenicity of SS to estimate the genotoxicity of ETS.
　Many refernces show that genotoxic compounds of environmental tobacco smoke will enter Human’s lung via adsorbing the aerosol. The study of this research discusses about SS of different formula type cigarettes, so, we prepare eight kinds of different flavor and formula cigarettes, collect the particulates from side-stream smoke on filters using a standard cigarette test method, each filter is then solvent extracted, cleaned up, concentrated, and then analyzed by a GC/MS for PAHs. Elemental analysis on selected metals will also be carried out. Each concentrated sample is then tested for genotoxicity with Ames Test. By camparing the result of this research, we can find out the genotoxicity of environmental tobacco smoke from different cigarettes.
　In conclusion, our results show that the aerosol in ETS is indirect- acting mutagen compounds, because they only show mutagenic reaction by adding S9 liver homogenate, and our data indicate that PAHs in ETS and the nitrogen content of tobacco are the most important factor of the SS genotoxicity. The SS caused by cigar has the strongest genotoxicity. SS result from cigarette made from England type has much PAHs than American type, cigarette made from American type of formula still has higher than England type if this two types of cigarettes has same “tar” content, because American type of formula contain more nitrogen element than England type. Light flavor cigarette which “tar” content is only half of dense flavor, but genotoxicity in SS just slight less than it. The adding of mint perfume will not affect genotoxicity in cigarette smoke.

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