本研究利用電性鑑別法應用於人類絨毛膜促性腺激素(Human Chorionic Gonadotropin, hCG)三明治免疫反應之檢測，結合阻抗式檢測電極晶片並利用金奈米粒子抗體標定技術，以助於偵測待測檢體之免疫反應並定量阻抗量測分析結果鑑別。實驗中利用電感、電容、電阻量測儀(LCR Meter)偵測阻抗晶片之電性訊號，藉由阻抗值改變分析判斷免疫反應變化，避免免疫色層分析法使用人眼辨別造成之誤差，並建立電性量測三明治免疫分析系統。為避免硝化纖維膜與檢測電極間間隙造成電阻抗訊號量測不穩定的狀態發生，直接將硝化纖維膜透過高分子溶液相轉換方式固定於檢測電極表面，成功利用阻抗變化、金奈米粒子標定技術和三明治免疫分析，達成進行人類絨毛膜促性腺激素(hCG)定量分析。其免疫檢測阻抗分析檢測試劑晶片之極限達6.25 mIU/mL，成功地突破檢測閾值25 mIU/mL限制，且電性阻抗與hCG濃度之檢量線決定係數(Coefficient of Determination)達0.9899，即表示本研究可利用此線性分析轉換曲線進行定量量測分析，且於免疫檢測分析上提供了一個全新的方向與思維。

This study, a novel electrode biochip for immunoassay was applied to detect sandwich-immunoassay of human chorionic gonadotropin, a gold nanoparticles (AuNPs) as a label of antibody to enhance immunoassay impedance quantitative analysis detection. Compared with traditional enzyme-linked immunosorbent assay (ELISA), we utilized the LCR meter instrument to detect the electric signal on electrode biochip. By detecting the impedance changes because of sandwich-immunoassay reaction, a sandwich-immunoassay detection system was established. In order to avoid a gap between a nitrocellulose membrane and electrode bringing about the unstable electric signal, a nitrocellulose membrane was fixed on a surface of electrode chip. In the study, combined impedance changing detection and gold nanoparticles labelled in sandwich-immunoassay achieves quantitative the concentration of human chorionic gonadotropin(hCG) antigen. The results show that the cut-off value decreased from 25 mIU/mL to 6.25 mIU/mL. The coefficient of determination between electric signal and concentration of hCG has come to 0.9899.

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