本文首先由單鏈的陰、陽離子界面活性劑製備雙鏈的DTMA-DS (dodecyltrimethylammonium-dodecylsulfate)、TTMA-DS (tetradecyltrimethylammonium- dodecylsulfate)與HTMA-DS (hexadecyltrimethylammonium-dodecylsulfate)三種陰陽離子界面活性劑（catanionic surfactant），連同PL-90磷脂質分別做為液胞的材料，並採用升溫半自發製程，嘗試製備穩定的陰陽體(陰陽離子液胞)與脂質體。由實驗結果顯示此製程並不適合製備脂質體，但透過膽固醇與離子型界劑的添加，DTMA-DS與HTMA-DS可以製備出穩定的陰陽離子液胞。本研究並進一步探討在純水與緩衝溶液環境下，液胞組成對界面電位、初始粒徑、物理穩定性、與溶液稀釋的影響。
此外，亦以螢光劑CF (5[6] -carboxyfluorescein)做為探測分子，探討陰陽體的包覆效率與釋放行為。實驗結果顯示，只有帶負電的陰陽體可以包覆CF，帶正電的陰陽體則會與帶負電的CF作用導致沉澱與相分離。且從管柱分離過程的結果發現帶負電的HTMA-DS陰陽體通過管柱後會呈現相分離的現象。此一現象透過界面電位與螢光分析顯示可能是SDS分子無法緊密地嵌HTMA-DS的雙層殼膜之間的緣故所造成。帶負電的DTMA-DS陰陽體經過管柱分離後仍有良好的穩定性，各組成陰陽體的包覆效率約在0.65~0.7% 之間。此外，實驗結果也顯示帶負電的DTMA-DS陰陽體在常溫下釋放速率非常小，但隨著溫度的提升而增大，且在50℃前後有很明顯的改變。此一結果可望提供陰陽體作為藥物傳輸載體溫控釋放的可行性。

The phospholipid PL-90 and ion-pair amphiphiles (HTMA-DS, TTMA-DS, and DTMA-DS), which are double-chained surfactants prepared through precipitation by mixing cationic and anionic single-chained surfactants, were used as the main materials to form liposomes and catanionic vesicles, respectively, by a semispontaneous process at 70 ℃. The experimental results showed that this method is not applicable to the preparation of liposome even by the addition of cholesterol. On the other hand, positively and negatively charged catanionic vesicles can be successfully prepared by using HTMA-DS and DTMA-DS with the addition of cholesterol and various amounts of single-chained ionic surfactants. Effects of composition on the size, zeta-potential, and physical stability of the ensuring catanionic vesicles in water and buffer solutions were systematically studied.
Furthermore, the encapsulation and release behavior of the ensuring catanionic vesicles were also investigated by using 5(6) -carboxyfluorescein (CF) as fluorescent probe molecules. The experimental results showed that only negatively charged catanionic vesicles are able to encapsulate CF molecules. However, CF molecules were unable to be encapsulated by positively charged catanionic vesicle. This is due to the precipitation resulted from the electrostatic intertation between the oppositely charged CF molecules and catanionic vesicles. Furthermore, negatively charged HTMA-DS catanionic vesicles can’t survive the size exclusion chromatography during separation process. On the evidence of zeta-potential and fluorescence measurements, this may be due to the SDS molecules without incorporation intact between HTMA-DS molecules in the bilayer structure of vesicles. Encapsulation efficiencies around 0.65~0.7% were determined and effect of temperature on release rate was studied for negatively charged DTMA-DS catanionic vesicles. The release rates were found to increase with the increase of temperature and there existed a drastic change of release rate around 50 ℃. This may find their potential use in temperature-controlled drug release by using catanionic vesicle as carriers.

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