本研究利用基因重組酵母菌檢測臺灣河川及工業園區廢水處理廠內外之類戴奧辛物質，並以Rec assay偵測具有高類戴奧辛活性或在高濃縮倍率下抑制酵母菌生長之樣本的基因毒性。此外，亦使用高效率液相層析儀分離具有高類戴奧辛活性和高基因毒性之樣本，以詳細調查河川水體、懸浮固體和底泥樣本中之類戴奧辛及基因毒性污染物，期能找出同時具有類戴奧辛活性和基因毒性之物質。
實驗結果顯示河川水體、懸浮固體或底泥樣本中均存在類戴奧辛物質。水體樣本BaP當量濃度介於70.0 - 10847.7 ng BaP-EQ/L，乾季朴子溪、將軍溪、二仁溪和阿公店溪，及濕季將軍溪、鹽水溪和阿公店溪受類戴奧辛物質污染較嚴重。懸浮固體樣本BaP當量濃度介於64.9 - 186041.1 ng BaP-EQ/L，乾季朴子溪牛稠溪橋、朴子大橋和二仁溪南萣橋，及濕季急水溪鐵線里和高屏溪里嶺大橋有較高之BaP當量濃度。河川底泥樣本BaP當量濃度介於340.0 - 36288.3 ng BaP/g-sediment-EQ，以淡水河和阿公店溪受到類戴奧辛物質污染較嚴重。經液相層析儀分離後，水體樣本、懸浮固體樣本、底泥樣本大多可在20至30分鐘收集之分段樣本中測得類戴奧辛活性。
具有高基因毒性或高類戴奧辛活性之水體、懸浮固體、及底泥樣本經由液相層析儀分離後，其分段樣本之基因毒性皆明顯下降，僅於二仁溪ER1與ER7濕季水體樣本之20至21分鐘分段樣本中測得較高之基因毒性，其中可能存在具有類戴奧辛活性及基因毒性之物質。

In this study, a recombinant yeast assay was used to detect dioxin-like contaminants in Taiwanese rivers and wastewater from industrial park. In addition, Rec assay was also used to detect genotoxicity in samples showing, high dioxin-like activity or yeast growth inhibition. These samples were further fractionated by high performance liquld (HPLC) to investigate potential substances exhibiting both dioxin-like activity and genotoxicity.
Experimental results showed that dioxin-like activity was detected in most of the water, suspended solids, and sediment samples. Benzo(a)pyrene equivalent concentration (BaP-EQ) of water samples was from 70.0 to 10847.7 ng BaP-EQ/L. Higher BaP-EQ was detected in PZ, JJ, ER and AGD rivers during the dry season, and JJ, YS and AGD rivers during the wet season. BaP-EQ of suspended solids samples was from 64.9 to 186041.1 ng BaP-EQ/L. Significant BaP-EQ was detected in PZ and ER rivers during the dry season, and JS and GP rivers during the wet season. BaP-EQ of sediment samples was from 340.0 to 36288.3 ng BaP/g sediment-EQ, and DS and AGD rivers were severely contaminated by dioxin-like compounds.
After HPLC fractionation, dioxin-like activity was mostly detected in fractions collected from 20 to 30 minutes. However, higher genotoxicity was only found in ER1 and ER7 water samples collected at Erren river during the wet season. Potential candidates showing dioxin-like activity and/or genotoxicity were present in the 21th fraction of ER1 and ER7 water samples.

參考文獻
1. 高肇藩 (ed.): 給水工程 (衛生工程 自來水篇); 1987.
2. 全國環境水質監測資訊網 [http://wqshow.epa.gov.tw/]. In.
3. 胡惠宇: 淡水河流域污染整治管理計畫. In.; 2008.
4. 賴慶昌: 98年度新竹縣頭前溪及鳳山溪流域生態治理區域水質監測暨成效評估計畫. In.; 2009.
5. 曾晴賢: 中港溪、客雅溪、南崁溪流域水環境生物指標監測教材及應用資料. In: 中港溪、客雅溪、南崁溪流域水環境生物指標監測教育研習會. Edited by 行政院環保署; 2003.
6. 秦詩漣: 九十三年度新竹縣 河川水質採樣、飲用水水質採樣研析計畫. In.: 新竹縣環境保護局; 2004.
7. 莊維修: 九十三年度新竹科學園區廢（污）水排放水稽查及管理計畫. In.: 新竹市環境保護局; 2004.
8. 蔡俊銘: 九十三年河川環境水體整體調查監測計畫[客雅溪(含三姓公溪)與北港溪流域]. In.; 2004.
9. 行政院環境保護署水質保護處、康城工程顧問公司、美商西圖工程顧問國際公司: 北港溪、朴子溪流域污染整治規劃 - 規劃總報告: 行政院環境保護署水質保護處; 1993.
10. 趙嘉雍: 「屏東縣流域及水污染稽查管制計畫」 -東港溪流域. In.: 屏東縣政府環境保護局; 2010.
11. 張大偉、簡傳彬: 應用生態工法整治河川污染調查研究計畫-以北港溪為例. In.: 行政院環境保護署、財團法人農業工程研究中心; 2008.
12. 勇興台、劉惠民: 99年度南部地區河川污染整治推動、輔導及評析計畫. In.: 行政院環境保護署; 2010.
13. 葉庭芳、蔡俊銘: 98年河川環境水體整體調查監測計畫. In.: 行政院源境保護署; 2009.
14. 高雄市環境保護局 [http://www.ksepb.gov.tw/ ]
15. 全球公民基金會 [http://met.ngo.org.tw/]
16. 吳豐帥、高志明、唐家慶: 高屏溪流域水污染源航照判釋及評析計畫. In., 行政院環境保護署 edn: 行政院環境保護署; 2009.
17. 高屏溪流域整治綱要檢討計畫(98-103年)規劃報告. In.: 經濟部水利署第七河川局; 2009.
18. 屏東縣高屏溪流域河川水質改善及污染削減具體行動計畫. In.: 屏東縣政府環境保護局; 2010.
19. 李澤民: 二仁溪及將軍溪流域污染削減執行計畫. In.: 水保處、財團法人中技社環境技術發展中心; 2002.
20. 李澤民: 二仁溪、將軍溪及鹽水溪流域污染削減執行計畫. In.: 水保處、財團法人中技社環境技術發展中心; 2003.
21. 洪瑋濃、林高弘: 南科液晶電視及產業支援工業區環境友善回饋計畫 - 周界環境監督計畫放流水成分及生物影響研究. In.; 2011.
22. Alok Bhandari RYS, Craig D. Adams, Pascale Champagne, Say Kee Ong, R. D. Tyagi and Tian C. Zhang (ed.): Contaminants of Emerging Environmental Concern; 2009.
23. Petrović M, Gonzalez S, Barceló D: Analysis and removal of emerging contaminants in wastewater and drinking water. TrAC Trends in Analytical Chemistry 2003, 22(10):685-696.
24. Radjenovic J, Petrovic M, Barceló D: Analysis of pharmaceuticals in wastewater and removal using a membrane bioreactor. Analytical and Bioanalytical Chemistry 2007, 387(4):1365-1377.
25. Bolong N, Ismail AF, Salim MR, Matsuura T: A review of the effects of emerging contaminants in wastewater and options for their removal. Desalination 2009, 239(1–3):229-246.
26. William Battaglin JD, Bret Bruce, and Mike McHugh: Contaminants of emerging concern in the environment. In: Water Resource IMPACT. vol. 9; 2007.
27. Macova M, Escher BI, Reungoat J, Carswell S, Chue KL, Keller J, Mueller JF: Monitoring the biological activity of micropollutants during advanced wastewater treatment with ozonation and activated carbon filtration. Water Research 2010, 44(2):477-492.
28. Castiglioni S, Bagnati R, Fanelli R, Pomati F, Calamari D, Zuccato E: Removal of Pharmaceuticals in Sewage Treatment Plants in Italy. Environmental Science & Technology 2005, 40(1):357-363.
29. Damià Barceló MP, Jelena Radjenovic: Treating emerging contaminants (pharmaceuticals) in wastewater and drinking water treatment plants. In: Technological Perspectives for Rational Use of Water Resources in the Mediterranean Region. 2009.
30. Dagnino S, Gomez E, Picot B, Cavaillès V, Casellas C, Balaguer P, Fenet H: Estrogenic and AhR activities in dissolved phase and suspended solids from wastewater treatment plants. Science of The Total Environment 2010, 408(12):2608-2615.
31. Reiner E, Clement R, Okey A, Marvin C: Advances in analytical techniques for polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and dioxin-like PCBs. Analytical and Bioanalytical Chemistry 2006, 386(4):791-806.
32. EPA. US: Health Assessment Document for Polychlorinated Dibenzo-P-Dioxins. In. Edited by Agency USEP. Washington; 1985.
33. EPA. U: Health Assessment Document for 2,3,7,8-Tetrachlorodibenzo-P-Dioxin (TCDD) and Related Compounds In. Edited by Agency USEP. Washington; 1994.
34. Birnbaum LS, Tuomisto J: Non-carcinogenic effects of TCDD in animals. Food Additives and Contaminants 2000, 17(4):275-288.
35. Kogevinas M: Human health effects of dioxins: cancer, reproductive and endocrine system effects. Human Reproduction Update 2001, 7(3):331-339.
36. Tohyama C: Low-Dose Exposure to Dioxin, Its Toxicities and Health Risk Assessment. Environ Sci 2002, 9:37-50.
37. Sciences TNAo: Health Risks from Dioxin and Related Compounds: Evaluation of the EPA Reassessment. In. Washington: The National Academics; 2006.
38. Whysner J, Williams GM: 2,3,7,8-Tetrachlorodibenzo-p-dioxin mechanistic data and risk assessment: Gene regulation, cytotoxicity, enhanced cell proliferation, and tumor promotion. Pharmacology & Therapeutics 1996, 71(1–2):193-223.
39. Poland A, Knutson JC: 2,3,7,8-Tetrachlorodibenzo-p-Dioxin and Related Halogenated Aromatic Hydrocarbons: Examination of the Mechanism of Toxicity. Annual Review of Pharmacology and Toxicology 1982, 22(1):517-554.
40. Schwarz M, Appel KE: Carcinogenic risks of dioxin: Mechanistic considerations. Regulatory Toxicology and Pharmacology 2005, 43(1):19-34.
41. Fernandez-Salguero PM, Hilbert DM, Rudikoff S, Ward JM, Gonzalez FJ: Aryl-hydrocarbon Receptor-Deficient Mice Are Resistant to 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Induced Toxicity. Toxicology and Applied Pharmacology 1996, 140(1):173-179.
42. Hagenmaier H, Lindig C, She J: Correlation of environmental occurrence of polychlorinated dibenzo-p-dioxins and dibenzofurans with possible sources. Chemosphere 1994, 29(9–11):2163-2174.
43. Kulkarni PS, Crespo JG, Afonso CAM: Dioxins sources and current remediation technologies — A review. Environment International 2008, 34(1):139-153.
44. Harrad SJ, Jones KC: A source inventory and budget for chlorinated dioxins and furans in the United Kingdom environment. Science of The Total Environment 1992, 126(1–2):89-107.
45. Bergknut M, Laudon H, Jansson S, Larsson A, Gocht T, Wiberg K: Atmospheric deposition, retention, and stream export of dioxins and PCBs in a pristine boreal catchment. Environmental Pollution 2011, 159(6):1592-1598.
46. Ahlborg UG, Brouwer A, Fingerhut MA, Jacobson JL, Jacobson SW, Kennedy SW, Kettrup AAF, Koeman JH, Poiger H, Rappe C et al: Impact of polychlorinated dibenzo-p-dioxins, dibenzofurans, and biphenyls on human and environmental health, with special emphasis on application of the toxic equivalency factor concept. European Journal of Pharmacology: Environmental Toxicology and Pharmacology 1992, 228(4):179-199.
47. 中華民國行政院環保署: 飲用水水質標準. In. Edited by 中華民國行政院環保署; 2001.
48. 中華民國行政院環保署: 土壤污染管制標準. In. Edited by 中華民國行政院環保署; 2011.
49. Hankinson O: The Aryl Hydrocarbon Receptor Complex. Annual Review of Pharmacology and Toxicology 1995, 35(1):307-340.
50. Mimura J, Fujii-Kuriyama Y: Functional role of AhR in the expression of toxic effects by TCDD. Biochimica et Biophysica Acta (BBA) - General Subjects 2003, 1619(3):263-268.
51. Schmidt JV, Su GH, Reddy JK, Simon MC, Bradfield CA: Characterization of a murine Ahr null allele: involvement of the Ah receptor in hepatic growth and development. Proceedings of the National Academy of Sciences 1996, 93(13):6731-6736.
52. Lahvis GP, Bradfield CA: ahr null alleles: distinctive or different? Biochemical Pharmacology 1998, 56(7):781-787.
53. Mimura J, Yamashita K, Nakamura K, Morita M, Takagi TN, Nakao K, Ema M, Sogawa K, Yasuda M, Katsuki M et al: Loss of teratogenic response to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice lacking the Ah (dioxin) receptor. Genes to Cells 1997, 2(10):645-654.
54. L F Bjeldanes JYK, K R Grose, J C Bartholomew, and C A Bradfield: Aromatic hydrocarbon responsiveness-receptor agonists generated from indole-3-carbinol in vitro and in vivo: comparisons with 2,3,7,8-tetrachlorodibenzo-p-dioxin. Proceedings of the National Academy of Sciences of the United States of America 1991.
55. Gillner M BJ, Cambillau C, Alexandersson M, Fernström B, Gustafsson JA.: Interactions of indolo[3,2-b]carbazoles and related polycyclic aromatic hydrocarbons with specific binding sites for 2,3,7,8-tetrachlorodibenzo-p-dioxin in rat liver. Molecular Pharmacology 1993.
56. Rannug U, Rannug A, Sjöberg U, Li H, Westerholm R, Bergman J: Structure elucidation of two tryptophan-derived, high affinity Ah receptor ligands. Chemistry & Biology 1995, 2(12):841-845.
57. Gradelet S, Astorg P, Leclerc J, Chevalier J, Vernevaut MF, Siess M-H: Effects of canthaxanthin, astaxanthin, lycopene and lutein on liver xenobiotic-metabolizing enzymes in the rat. Xenobiotica 1996, 26(1):49-63.
58. Washburn BS, Rein KS, Baden DG, Walsh PJ, Hinton DE, Tullis K, Denison MS: Brevetoxin-6 (PbTx-6), a Nonaromatic Marine Neurotoxin, Is a Ligand of the Aryl Hydrocarbon Receptor. Archives of Biochemistry and Biophysics 1997, 343(2):149-156.
59. Kleman MI, Övervik E, Mason GGF, Gustafsson J-Å: In vitro activation of the dioxin receptor to a DNA-binding form by food-borne heterocyclic amines. Carcinogenesis 1992, 13(9):1619-1624.
60. Bittner M, Hilscherová K, Giesy JP: Changes of AhR-mediated activity of humic substances after irradiation. Environment International 2007, 33(6):812-816.
61. Jeuken A, Keser BJG, Khan E, Brouwer A, Koeman J, Denison MS: Activation of the Ah Receptor by Extracts of Dietary Herbal Supplements, Vegetables, and Fruits. Journal of Agricultural and Food Chemistry 2003, 51(18):5478-5487.
62. Amakura Y, Tsutsumi T, Nakamura M, Kitagawa H, Fujino J, Sasaki K, Yoshida T, Toyoda M: Preliminary Screening of the Inhibitory Effect of Food Extracts on Activation of the Aryl Hydrocarbon Receptor Induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Biological and Pharmaceutical Bulletin 2002, 25(2):272-274.
63. Hilscherova K, Machala M, Kannan K, Blankenship A, Giesy J: Cell bioassays for detection of aryl hydrocarbon (AhR) and estrogen receptor (ER) mediated activity in environmental samples. Environmental Science and Pollution Research 2000, 7(3):159-171.
64. Giesy JP, Hilscherova K, Jones PD, Kannan K, Machala M: Cell bioassays for detection of aryl hydrocarbon (AhR) and estrogen receptor (ER) mediated activity in environmental samples. Marine Pollution Bulletin 2002, 45(1–12):3-16.
65. Delgado-Saborit JM, Stark C, Harrison RM: Carcinogenic potential, levels and sources of polycyclic aromatic hydrocarbon mixtures in indoor and outdoor environments and their implications for air quality standards. Environment International 2011, 37(2):383-392.
66. Collins JF, Brown JP, Alexeeff GV, Salmon AG: Potency Equivalency Factors for Some Polycyclic Aromatic Hydrocarbons and Polycyclic Aromatic Hydrocarbon Derivatives. Regulatory Toxicology and Pharmacology 1998, 28(1):45-54.
67. Schneider K, Roller M, Kalberlah F, Schuhmacher-Wolz U: Cancer risk assessment for oral exposure to PAH mixtures. Journal of Applied Toxicology 2002, 22(1):73-83.
68. MACKENZIE KM, ANGEVINE DM: Infertility in Mice Exposed in utero to Benzo(a)pyrene. Biology of Reproduction 1981, 24(1):183-191.
69. Penn A BG, Soloman J, Burns F, Albert R.: Dose-dependent size increases of aortic lesions following chronic exposure to 7,12-dimethylbenz(a)anthracene. Cancer Res 1981:588-592.
70. Paigen B, Havens MB, Morrow A: Effect of 3-Methylcholanthrene on the Development of Aortic Lesions in Mice. Cancer Research 1985, 45(8):3850-3855.
71. Hardin JA, Hinoshita F, Sherr DH: Mechanisms by which benzo[a]pyrene, an environmental carcinogen, suppresses B cell lymphopoiesis. Toxicology and Applied Pharmacology 1992, 117(2):155-164.
72. Armstrong EC, Bonser GM: Squamous carcinoma of the fore-stomach and other lesions in mice following oral administration of 3:4:5:6-dibenzcarbazole. British journal of cancer 1950, 4(2):203-211.
73. Menzie CA, Potocki BB, Santodonato J: Exposure to carcinogenic PAHs in the environment. Environmental Science & Technology 1992, 26(7):1278-1284.
74. Suarez MP, Rifai HS, Palachek R, Dean K, Koenig L: Distribution of polychlorinated dibenzo-p-dioxins and dibenzofurans in suspended sediments, dissolved phase and bottom sediment in the Houston Ship Channel. Chemosphere 2006, 62(3):417-429.
75. Lohmann R, Nelson E, Eisenreich SJ, Jones KC: Evidence for Dynamic Air−Water Exchange of PCDD/Fs:  A Study in the Raritan Bay/Hudson River Estuary. Environmental Science & Technology 2000, 34(15):3086-3093.
76. Dag Broman CN, Carl Rolff, Yngve Zebuhr: Occurrence and Dynamics of Polychlorinated Dibenzo-p -dioxins and Dibenzofurans and Polycyclic Aromatic Hydrocarbons in the Mixed Surface Layer of Remote Coastal and Offshore Waters of the Baltic. Environ Sci Technol 1991, 25:1850-1864.
77. Kakimoto H, Oka H, Miyata Y, Yonezawa Y, Niikawa A, Kyudo H, Tang N, Toriba A, Kizu R, Hayakawa K: Homologue and isomer distribution of dioxins observed in water samples collected from Kahokugata Lagoon and inflowing rivers, Japan. Water Research 2006, 40(10):1929-1940.
78. Liu Y, Peng Pa, Li X, Zhang S, Ren M: Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in water and suspended particulate matter from the Xijiang River, China. Journal of Hazardous Materials 2008, 152(1):40-47.
79. Götz R, Enge P, Friesel P, Roch K, Kjeller LO, Kulp SE, Rappe C: Sampling and analysis of water and suspended particulate matter of the river Elbe for polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs). Chemosphere 1994, 28(1):63-74.
80. Thuan NT, Tsai CL, Weng YM, Lee TY, Chang MB: Analysis of polychlorinated dibenzo-p-dioxins and furans in various aqueous samples in Taiwan. Chemosphere 2011, 83(6):760-766.
81. Kiguchi O, Kobayashi T, Wada Y, Saitoh K, Ogawa N: Polychlorinated dibenzo-p-dioxins and dibenzofurans in paddy soils and river sediments in Akita, Japan. Chemosphere 2007, 67(3):557-573.
82. Kim K-S, Lee SC, Kim K-H, Shim WJ, Hong SH, Choi KH, Yoon JH, Kim J-G: Survey on organochlorine pesticides, PCDD/Fs, dioxin-like PCBs and HCB in sediments from the Han river, Korea. Chemosphere 2009, 75(5):580-587.
83. Maskaoui K, Zhou JL, Hong HS, Zhang ZL: Contamination by polycyclic aromatic hydrocarbons in the Jiulong River Estuary and Western Xiamen Sea, China. Environmental Pollution 2002, 118(1):109-122.
84. Chen B, Xuan X, Zhu L, Wang J, Gao Y, Yang K, Shen X, Lou B: Distributions of polycyclic aromatic hydrocarbons in surface waters, sediments and soils of Hangzhou City, China. Water Research 2004, 38(16):3558-3568.
85. Shi Z, Tao S, Pan B, Fan W, He XC, Zuo Q, Wu SP, Li BG, Cao J, Liu WX et al: Contamination of rivers in Tianjin, China by polycyclic aromatic hydrocarbons. Environmental Pollution 2005, 134(1):97-111.
86. Guo W, He M, Yang Z, Lin C, Quan X, Wang H: Distribution of polycyclic aromatic hydrocarbons in water, suspended particulate matter and sediment from Daliao River watershed, China. Chemosphere 2007, 68(1):93-104.
87. Zhang S, Zhang Q, Darisaw S, Ehie O, Wang G: Simultaneous quantification of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and pharmaceuticals and personal care products (PPCPs) in Mississippi river water, in New Orleans, Louisiana, USA. Chemosphere 2007, 66(6):1057-1069.
88. Countway RE, Dickhut RM, Canuel EA: Polycyclic aromatic hydrocarbon (PAH) distributions and associations with organic matter in surface waters of the York River, VA Estuary. Organic Geochemistry 2003, 34(2):209-224.
89. Ross JRM, Oros DR: Polycyclic aromatic hydrocarbons in the San Francisco Estuary water column: Sources, spatial distributions, and temporal trends (1993–2001). Chemosphere 2004, 57(8):909-920.
90. Fernandes MB, Sicre MA, Boireau A, Tronczynski J: Polyaromatic hydrocarbon (PAH) distributions in the Seine River and its estuary. Marine Pollution Bulletin 1997, 34(11):857-867.
91. Doong R-a, Lin Y-t: Characterization and distribution of polycyclic aromatic hydrocarbon contaminations in surface sediment and water from Gao-ping River, Taiwan. Water Research 2004, 38(7):1733-1744.
92. Luo X-J, Chen S-J, Mai B-X, Yang Q-S, Sheng G-Y, Fu J-M: Polycyclic aromatic hydrocarbons in suspended particulate matter and sediments from the Pearl River Estuary and adjacent coastal areas, China. Environmental Pollution 2006, 139(1):9-20.
93. Louiz I, Kinani S, Gouze ME, Ben-Attia M, Menif D, Bouchonnet S, Porcher JM, Ben-Hassine OK, Aït-Aïssa S: Monitoring of dioxin-like, estrogenic and anti-androgenic activities in sediments of the Bizerta lagoon (Tunisia) by means of in vitro cell-based bioassays: Contribution of low concentrations of polynuclear aromatic hydrocarbons (PAHs). Science of The Total Environment 2008, 402(2-3):318-329.
94. Koh CH, Khim JS, Kannan K, Villeneuve DL, Senthilkumar K, Giesy JP: Polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs) and 2,3,7,8-TCDD equivalents (TEQs) in sediment from the Hyeongsan River, Korea. Environmental Pollution 2004, 132(3):489-501.
95. Suares Rocha P, Azab E, Schmidt B, Storch V, Hollert H, Braunbeck T: Changes in toxicity and dioxin-like activity of sediments from the Tietê River (São Paulo, Brazil). Ecotoxicology and Environmental Safety 2010, 73(4):550-558.
96. Kinani S, Bouchonnet S, Creusot N, Bourcier S, Balaguer P, Porcher J-M, Aït-Aïssa S: Bioanalytical characterisation of multiple endocrine- and dioxin-like activities in sediments from reference and impacted small rivers. Environmental Pollution 2010, 158(1):74-83.
97. Li AP, Heflich RH (eds.): Genetic toxicology; 1991.
98. Tiana L, Caib Q, Wei H: Alterations of Antioxidant Enzymes and Oxidative Damage to Macromolecules in Different Organs of Rats During Aging. Free Radical Biology and Medicine 1998, 24(9):1477-1484.
99. Drabløs F, Feyzi E, Aas PA, Vaagbø CB, Kavli B, Bratlie MS, Peña-Diaz J, Otterlei M, Slupphaug G, Krokan HE: Alkylation damage in DNA and RNA—repair mechanisms and medical significance. DNA Repair 2004, 3(11):1389-1407.
100. Spencer JPE, Whiteman M, Jenner A, Halliwell B: Nitrite-induced deamination and hypochlorite-induced oxidation of DNA in intact human respiratory tract epithelial cells. Free Radical Biology and Medicine 2000, 28(7):1039-1050.
101. Kent C (ed.): Basics of toxicology; 1998.
102. Friedberg EC: DNA damage and repair. Nature 2003, 421:436-440.
103. COOKE MS, EVANS MD, DIZDAROGLU M, LUNEC J: Oxidative DNA damage: mechanisms, mutation, and disease. The FASEB Journal 2003, 17(10):1195-1214.
104. Severin I, Dumont C, Jondeau-Cabaton A, Graillot V, Chagnon M-C: Genotoxic activities of the food contaminant 5-hydroxymethylfurfural using different in vitro bioassays. Toxicology Letters 2010, 192(2):189-194.
105. Combes RD, Haveland-Smith RB: A review of the genotoxicity of food, drug and cosmetic colours and other azo, triphenylmethane and xanthene dyes. Mutation Research/Reviews in Genetic Toxicology 1982, 98(2):101-243.
106. Sevastyanova O, Binkova B, Topinka J, Sram RJ, Kalina I, Popov T, Novakova Z, Farmer PB: In vitro genotoxicity of PAH mixtures and organic extract from urban air particles: Part II: Human cell lines. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 2007, 620(1–2):123-134.
107. Sevastyanova O, Novakova Z, Hanzalova K, Binkova B, Sram RJ, Topinka J: Temporal variation in the genotoxic potential of urban air particulate matter. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2008, 649(1–2):179-186.
108. Sánchez-Guerra M, Pelallo-Martínez N, Díaz-Barriga F, Rothenberg SJ, Hernández-Cadena L, Faugeron S, Oropeza-Hernández LF, Guaderrama-Díaz M, Quintanilla-Vega B: Environmental polycyclic aromatic hydrocarbon (PAH) exposure and DNA damage in Mexican children. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2012, 742(1–2):66-71.
109. Liu J-R, Pang Y-X, Tang X-L, Dong H-W, Chen B-Q, Sun C-H: Genotoxic activity of organic contamination of the Songhua River in the north-eastern region of the People's Republic of China. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2007, 634(1–2):81-92.
110. Fatima RA, Ahmad M: Genotoxicity of industrial wastewaters obtained from two different pollution sources in northern India: A comparison of three bioassays. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2006, 609(1):81-91.
111. Žegura B, Heath E, Černoša A, Filipič M: Combination of in vitro bioassays for the determination of cytotoxic and genotoxic potential of wastewater, surface water and drinking water samples. Chemosphere 2009, 75(11):1453-1460.
112. Delgado-Rodriguez A, Ortíz-Marttelo R, Graf U, Villalobos-Pietrini R, Gómez-Arroyo S: Genotoxic activity of environmentally important polycyclic aromatic hydrocarbons and their nitro derivatives in the wing spot test of Drosophila melanogaster. Mutation Research/Genetic Toxicology 1995, 341(4):235-247.
113. Tuominen J, Salomaa S, Pyysalo H, Skytta E, Tikkanen L, Nurmela T, Sorsa M, Pohjola V, Sauri M, Himberg K: Polynuclear aromatic compounds and genotoxicity in particulate and vapor phases of ambient air: effect of traffic, season, and meteorological conditions. Environmental Science & Technology 1988, 22(10):1228-1234.
114. Garganta F, Krause G, Scherer G: Base-substitution profiles of externally activated polycyclic aromatic hydrocarbons and aromatic amines determined in a lacZ reversion assay. Environmental and Molecular Mutagenesis 1999, 33(1):75-85.
115. Behnisch PA, Hosoe K, Sakai S-i: Bioanalytical screening methods for dioxins and dioxin-like compounds — a review of bioassay/biomarker technology. Environment International 2001, 27(5):413-439.
116. Mark E H: Biomarkers and bioassays for detecting dioxin-like compounds in the marine environment. Science of The Total Environment 2002, 289(1-3):49-69.
117. Poland CABaA: A competitive binding assay for 2, 3, 7, 8-tetraxhlorodibenzo-p-dioxin and related ligand of the Ah receptor. Molecular Pharmacology 1988.
118. Yao EF, Denison MS: DNA sequence determinants for binding of transformed Ah receptor to a dioxin-responsive enhancer. Biochemistry 1992, 31(21):5060-5067.
119. Sawyer T, Safe S: PCB isomers and congeners: Induction of aryl hydrocarbon hydroxylase and ethoxyresorufin O-deethylase enzyme activities in rat hepatoma cells. Toxicology Letters 1982, 13(1–2):87-93.
120. Petrulis JR, Chen G, Benn S, LaMarre J, Bunce NJ: Application of the ethoxyresorufin-O-deethylase (EROD) assay to mixtures of halogenated aromatic compounds. Environmental Toxicology 2001, 16(2):177-184.
121. Murk AJ LJ, Denison MS, Giesy JP, van de Guchte C, Brouwer A.: Chemical-activated luciferase gene expression (CALUX): a novel in vitro bioassay for Ah receptor active compounds in sediments and pore water. FUNDAMENTAL AND APPUED TOXICOLOGY 1996, 33:149-160.
122. GARRISON PM, TULLIS K, AARTS JMMJG, BROUWER A, GIESY JP, DENISON MS: Species-Specific Recombinant Cell Lines as Bioassay Systems for the Detection of 2,3,7,8-Tetrachlorodibenzo-p-dioxin-like Chemicals. Toxicological Sciences 1996, 30(2):194-203.
123. Scott R. Nagy JRS, Bruce D. Hammock, and Michael S. Denison: Development of a Green Fluorescent Protein-Based Cell Bioassay for the Rapid and Inexpensive Detection and Characterization of Ah Receptor Agonists. Toxicological Sciences 2001, 65:200-210.
124. Nagy SR, Liu G, Lam KS, Denison MS: Identification of Novel Ah Receptor Agonists Using a High-Throughput Green Fluorescent Protein-Based Recombinant Cell Bioassay†. Biochemistry 2001, 41(3):861-868.
125. Ames BN, McCann J, Yamasaki E: Carcinogens are mutagens: A simple test system. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 1975, 33(1):27-28.
126. Green MHL, Muriel WJ: Mutagen testing using TRP+ reversion in Escherichia coli. Mutation Research/Environmental Mutagenesis and Related Subjects 1976, 38(1):3-32.
127. David J. Brusick VFS, Herbert S. Rosenkranz, Verne A. Ray and Robert S. Stafford: An evaluation of the Escbericbia coli WP2 and WP2 uvrA reverse mutation assay. Mutation Research 1980, 76:169-190.
128. Thomas Johnson B: An evaluation of a genotoxicity assay with liver s9 for activation and luminescent bacteria for detection. Environmental Toxicology and Chemistry 1992, 11(4):473-480.
129. Hauser B, Schrader G, Bahadir M: Dependence of Genotoxicity of Benzo[a]pyrene Suspensions in Mutatox Test on Dissolved Concentration and S9 Addition. Ecotoxicology and Environmental Safety 1997, 38(3):224-226.
130. Békaert C, Rast C, Ferrier V, Bispo A, Jourdain MJ, Vasseur P: Use of in vitro (Ames and Mutatox tests) and in vivo (Amphibian Micronucleus test) assays to assess the genotoxicity of leachates from a contaminated soil. Organic Geochemistry 1999, 30(8, Part 2):953-962.
131. Girotti S, Ferri EN, Fumo MG, Maiolini E: Monitoring of environmental pollutants by bioluminescent bacteria. Analytica Chimica Acta 2008, 608(1):2-29.
132. Hideo S, Takeshi K, Tsuneo I, Kozo M, Atsuo N: Cloning and characterization of the umu operon responsible for inducible mutagenesis in Escherichia coli. Gene 1983, 23(2):167-174.
133. Oda Y, Nakamura S-i, Oki I, Kato T, Shinagawa H: Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens. Mutation Research/Environmental Mutagenesis and Related Subjects 1985, 147(5):219-229.
134. Fairbairn DW, Olive PL, O'Neill KL: The comet assay: a comprehensive review. Mutation Research/Reviews in Genetic Toxicology 1995, 339(1):37-59.
135. R. R. Tice EA, D. Anderson, B. Burlinson, A. Hartmann,, H. Kobayashi YM, E. Rojas, J.-C. Ryu, and Y. F. Sasaki: Single Cell Gel/Comet Assay: Guidelines for In Vitro and In Vivo Genetic Toxicology Testing. Environmental and Molecular Mutagenesis 2000, 35:206-221.
136. Miller Iii CA: A Human Aryl Hydrocarbon Receptor Signaling Pathway Constructed in Yeast Displays Additive Responses to Ligand Mixtures. Toxicology and Applied Pharmacology 1999, 160(3):297-303.
137. Kawanishi M, Sakamoto M, Ito A, Kishi K, Yagi T: Construction of reporter yeasts for mouse aryl hydrocarbon receptor ligand activity. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2003, 540(1):99-105.
138. Rodbard D (ed.): Mathematics and statistics of ligand assay: An illustrated guide. New York: Masson; 1981.
139. Kada T, Tutikawa K, Sadaie Y: In vitro and host-mediated “rec-assay” procedures for screening chemical mutagens; and phloxine, a mutagenic red dye detected. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 1972, 16(2):165-174.
140. Takigami H, Matsui S, Matsuda T, Shimizu Y: The Bacillus subtilis rec-assay: a powerful tool for the detection of genotoxic substances in the water environment. Prospect for assessing potential impact of pollutants from stabilized wastes. Waste Management 2002, 22(2):209-213.
141. Oksuzoglu E, Temiz-Arpaci O, Tekiner-Gulbas B, Eroglu H, Sen G, Alper S, Yildiz I, Diril N, Aki-Sener E, Yalcin I: A study on the genotoxic activities of some new benzoxazoles. Medicinal Chemistry Research 2008, 16(1):1-14.
142. Misaki K, Kawami H, Tanaka T, Handa H, Nakamura M, Matsui S, Matsuda T: Aryl hydrocarbon receptor ligand activity of polycyclic aromatic ketones and polycyclic aromatic quinones. Environmental Toxicology and Chemistry 2007, 26(7):1370-1379.
143. Kim H-k, Masaki H, Matsumura T, Kamei T, Magara Y: Removal efficiency and homologue patterns of dioxins in drinking water treatment. Water Research 2002, 36(19):4861-4869.
144. Smirnov AD, Schecter A, Päpke O, Beljak AA: Conclusions from Ufa, Russia, drinking water dioxin cleanup experiments involving different treatment technologies. Chemosphere 1996, 32(3):479-489.