導引骨再生為口腔顎面外科重建骨缺損之重要治療方式，臨床上多仰賴骨移植材料搭配再生膜與鈦網以維持再生空間。然而，近年文獻指出過度的細胞阻隔可能影響血管新生，且再生膜與鈦網本身仍存在暴露風險、需二次手術移除等臨床限制。富含血小板纖維蛋白所製備之黏性骨因兼具良好操作性、空間維持能力及生長因子釋放潛能，已被廣泛應用於骨缺損重建，並在部分情況下可不依賴再生膜而達成良好臨床效果。然而，現行黏性骨製備方式仍存在凝固時間較長、生長因子分布不均、力學性質受限等問題。
台灣齒列再生研究學會基於既有臨床成果，提出一種創新改良之黏性骨製備策略，稱為複合再生基質。此新創方法透過整合自體纖維蛋白原膠與固態富血小板纖維蛋白壓榨液中之凝血酶，並藉由多次混合與重塑步驟，使骨移植顆粒表面可均勻浸潤高濃度生長因子，同時避免傳統剪碎富血小板纖維蛋白所可能引發之纖維蛋白結構破壞，可以提升材料之凝固效率與結構穩定性。
現存的問題是：複合再生基質所號稱的優點僅是臨床結果與使用經驗所衍伸出的推測，未經基礎研究數據證實。
本研究之目的分為兩大目標：其一，分析固態富血小板纖維蛋白壓榨液、自體纖維蛋白原膠及其混合物的差異；其二，以基礎研究證實台灣齒列再生研究學會所提出之複合再生基質的優點。
材料與方法如下：自血液捐贈者採集靜脈血液，製成固態富血小板纖維蛋白壓榨液、自體纖維蛋白原膠並定量其凝血因子與生長因子，並測量自體纖維蛋白原膠與兩者的混合液的凝固時間與凝塊的抗壓強度。以三種作法製備出黏性骨並定量其生長因子、孔隙率與抗壓強度。以 MG-63 細胞評估三種黏性骨的細胞增生與細胞分化能力。
研究結果表明，固態富血小板纖維蛋白壓榨液富含凝血酶，自體纖維蛋白原膠富含纖維蛋白原與多種生長因子。固態富血小板纖維蛋白壓榨液可以加快凝固速度與凝塊的抗壓強度。複合再生基質可以提升骨粉的填充率以達到減少孔隙率，且抗壓強度也顯著高於其他製備方法；但在生長因子的釋放與細胞實驗中並沒有得到提升，反而是降低，顯示固態富血小板纖維蛋白壓榨液的加入可能導致所含的生長因子被稀釋。
總結來說，本研究闡明固態富血小板纖維蛋白壓榨液與自體纖維蛋白原膠的成分與交互作用效果，而複合再生基質的做法可以提升其機械性質，但是沒有提升其生物性質。

To overcome the limitations of existing bone graft materials in osteogenesis and mechanical stability, this study evaluated the biological and biomechanical performance of sticky bone, with particular emphasis on a novel formulation termed the Composite Regenerative Matrix (CRM). Three preparation strategies were systematically compared: a conventional method combining autologous fibrin glue (AFG) with hydroxyapatite (HA), an updated method incorporating fragmented platelet-rich fibrin (PRF) membranes, and the CRM approach produced by repeated kneading of AFG, PRF serum, and HA to induce multi-step fibrin network formation. Biochemical analyses demonstrated that AFG contained abundant fibrinogen and growth factors, whereas PRF serum exhibited significantly higher thrombin levels, contributing to accelerated coagulation and enhanced clot strength when combined with AFG. Mechanical testing and micro-computed tomography revealed that CRM achieved the highest compressive strength, the most homogeneous HA particle distribution, and a well-organized pore architecture among all groups. In vitro assays using MG-63 osteoblast-like cells confirmed the biocompatibility of all formulations, with CRM showing superior or comparable effects on cell proliferation, TGF-β1 secretion, and early osteogenic differentiation. Collectively, these findings indicate that while conventional and updated methods offer procedural simplicity and rapid moldability, respectively, CRM provides enhanced mechanical stability and structural integrity, making it particularly suitable for large bone defect reconstruction requiring sustained support.

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