將選擇性乙醯基化之γ環糊精以3-glycidoxypropyltrimethoxy silane (GPTMOS)之矽烷化合物鍵結於粒徑5μm矽膠粒子上，將之充填入100μm內徑融矽石英管柱並藉溶膠-凝膠反應製備成整體管柱。利用高效率液相層析及毛細管電層析對多環芳香族分離混合物、1-phenylethane-1,2-diol及 dansyl serine對掌異構物化合物進行分離。結果顯示對多環芳香族滯留時間因分析物於動相之溶解度及主-客錯合物穩定度造成不同滯留順序。對於1-phenylethanol， 1-phenylethane-1,2-diol及 dansyl serine對掌異構物化合物而言，1-phenylethanol 於2,3-乙醯基化之γ環糊精較2,3-乙醯基化之β環糊精有較好解析，1-phenylethane-1, 2diol於2,3-及2-乙醯基化之γ環糊精則顯示不同解析能力，dansyl serine於γ環糊精則具有基線解析。相較於β環糊精，γ環糊精空腔較大且具彈性結構有助於乙醯基化之γ環糊精解析對掌異構物化合物。

　　Native and selectively acetylated γ-cyclodextrins were synthesized as chiral stationary phases. Compound, 3-glycidoxypropyltrimethoxy silane (GPTMOS), is used to modify the hydroxyl groups of 6-positions of γ-cylcodextrins for bonding on silica gel.A piece of 100 I.D.μm fused silica capillary (10 to 20 ㎝) was packed with thoses particles, and then proceeding sol-gel reaction to produce monolithic columns for CEC an μ-HPLC.

　　Results show different retention order for polyaromatic hydrocarbons (PAHs) influenced by the stability of analytes-CDs inclusion complex, solubility of analytes in mobile phase. Results also show dansyl serine with native γ-cyclodextrin has baseline resolution which is better than native β- cyclodextrin. 1-phenylethanol was better resolved with 2, 3-O-diacetyl-γ-cyclodextrin than 2, 3-O-diacetyl-β- cyclodextrin. 1-phenylethane-1, 2diol was resolved by native γ-cyclodextrin, 2, 3-O-diacetyl-γ-cyclodextrin and 2-O-acetyl-γ-cyclodextrin.

　　It is due to the fact that γ-cyclodextrin has the wider cavity width and more flexible than β-cyclodextyrin, which the modification of acetyl groups – more stereo than methyl at different positions of hydroxyl groups of γ-cycyclodextrin facilitate chiral recognition.

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