微流道晶片技術之發展有以下數個優點，包含試劑與樣本上的使用量少、反應時間與分析時間縮短、高通量和可攜帶性。我們先前在糖尿病視網膜的診斷中成功地偵測到蛋白質，如淚脂質運載蛋白(LCN-1)和血管內皮生長因子(VEGF)。該研究利用光電動技術抓取目標抗原及免疫螢光粒子增強，形成微珠式免疫分析法。在免疫分析後螢光訊號的出現暗指有成功的三明治免疫複合物。然而分布稀疏的粒子僅會產生微弱的螢光訊號。為了克服這個缺點，一種有效的光電動方法，採用快速電動圖紋法(REP)聚集粒子達到免疫螢光增強的目的。此外，透過結合快速圖紋法、磁珠及開放式的孔洞，改善由高介質導電度所導致的快速電動圖紋法之限制。這項技術上的結合能克服先前技術的缺點。在本篇研究，針對一種在很多疾病中常見的細胞激素:腫瘤壞死因子，我們提出了快速免疫感測方法。免疫分析法裡使用磁性粒子可以幫助維持電導度，並在開放式孔洞的操作下提供主要的優勢。和捕獲抗體接合的磁性粒子製備樣本直接加入微孔中，達成目標抗原捕獲。形成三明治結構後，粒子必定散發螢光，標示為成功地抓取目標抗原。一旦免疫分析完成，使用微流道晶片底下的磁鐵重複沖洗。為了降低樣本的導電度，將磁性粒子懸浮於去離子水中。
免疫螢光增強結果指出在快速電動圖紋法中高出了約5.6倍的讀取值。除了得益於免疫分析法，受到此技術帶動，螢光訊號在1分鐘內達到最大值並產生2.9 pg/mL 的檢測極限。人體的淚液樣本在實際測試中是利用發展佳的裝置及利用酵素結合免疫吸附分析法(ELISA)去驗證。趨勢上兩者有達到良好的一致性。開放性孔洞裝置提供在未來簡化臨床診斷一個前景。微流道晶片結合免疫分析法的主要目的是藉由省去繁雜且耗時的工作流程，達到降低操作不易性。

Developing a lab-on-chip technology has numerous advantages, including significant reduction of reagents and sample volume, short reaction and analysis time, high throughput, and portability. Previously, we had effectively identified proteins, like Lipocalin-1 (LCN-1) and Vascular Endothelial Growth Factor (VEGF), for diabetic retinopathy diagnosis. Diabetic retinopathy diagnosis constituted a bead-based immunoassay for target capture with a combination of immunofluorescence enhancement with an optoelectrokinetic technique. The fluorescent signal after the immunoassay implies a successful sandwiched immunocomplex. However, sparsely distributed particles yield low and poor fluorescent signals. To overcome the shortcoming, a powerful optoelectrokinetic tool, Rapid Electrokinetic Patterning (REP), was adopted here to concentrate the particles for immunofluorescence enhancement. In addition, we improve the limitations of REP resulting from high, medium conductivity by integrating the REP with magnetic particles and an open well. The combination enables overcoming the drawbacks of the previous technique. This study proposes rapid immuno-sensing, a specific cytokine biomarker found in many diseases, tumor necrosis factor. Magnetic particles utilized in immunoassay can help maintain conductivity and supply a significant advantage in open well operation. A preprepared sample of magnetic particles conjugated with capture antibody is directed into well for target antigen capture. After forming the sandwiched structure, the particle must emit fluorescence to mark successful target capture. Once the immunoassay is complete, we perform repeated on-site washing with a magnet placed at the bottom of the chip. Magnetic particles are then suspended in deionized water to promote the reduction in sample conductivity. The immunofluorescence enhancement results exhibited about 5.6X times better read-out with REP. In addition to benefits from immunoassays, the fluorescent signal driven by the technique reached its maximum in 1 min and yielded a limit of detection at 2.9 pg/mL. For a practical test, the developed device measured human tear samples and validated them with a standard ELISA. Both data showed good agreement in trend. The open-well device provides insight into future simplistic clinical diagnoses. The primary purpose of immunoassay into lab-on-chip is to ease the labor by cutting several tedious and time-consuming work procedures.

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