本研究是以一種新穎分析方法，依據界面活性劑的特性，結合高性能液相色譜分析(HPLC)法，嘗試萃取水相中的多苯環芳香烴碳水化合物(polycyclic aromatic hydrocarbons，PAHs)進行微量分析，並同時探討界面活性劑相行為和鹽類的添加對於萃取程序的影響及預濃縮因子(preconcentration)、回收效率等參數之變化關係。在此研究中，我們嘗試以界面活性劑的液晶相進行萃取水中六種含有2至4個環狀結構的微量多苯環芳香烴碳水化合物。所運用的界面活性劑以Brij 30 (也就是C12E4)為主。在研究方面，我們也比較、整理出溫度效應、加鹽效應、界面活性劑之濃度以及分析物初濃度等等效應對相分離萃取程序之影響和種種變因控制之探討，且在預濃縮效果及萃取回收效率(recovery efficiency)方面顯示了非常不錯的結果。

　　A novel but simple surfactant-based extraction process is developed to preconcentrate the trace of selected polycyclic aromatic hydrocarbons (PAHs) with the use of high performance liquid chromatography (HPLC) for the separation and the micro analysis. This study investigated the effects of the surfactant phase behavior, additives; and the influences on the phase-volume ratio, preconcentration factor, and recovery efficiency. These compounds are ubiquitous in the environment and are released from anthropogenic or natural origins (for example: the sea water, the river water, the soil, the industrial wastes, the ashes, or the solid wastes), with the use of the readily biodegradable nonionic surfactant Brij 30 as extractant. The concentrations of PAHs mixtures in the spiked samples were determined with such phase-separation extraction process at different practical temperature (30~50oC) followed by HPLC with the UV detection. No special washing step is, thus, required to remove surfactant before HPLC analysis. And it shows as a good result, satisfied recovery percentages were reached.

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