本論文係有關NaYF4:Yb,Er/二氧化矽/阿拉伯膠近紅外光上轉換複合奈米粒子之製備及作為兼具螢光顯影與藥物釋放功能之生醫奈米載體的應用研究。關於其製備，首先利用奧士瓦熟成法合成NaYF4:Yb,Er奈米粒子，接著以溶凝膠法在逆微胞系統中被覆二氧化矽奈米殼層在NaYF4:Yb,Er奈米粒子表面，製得NaYF4:Yb,Er-SiO2核殼型奈米粒子(NaYF4:Yb,Er@SiO2)。接著，以(3-氨基丙基)三乙氧基矽烷將二氧化矽表面修飾成具有氨基，最後再將阿拉伯膠(GA)以碳二醯胺活化法接枝在氨基化之二氧化矽表面，製得阿拉伯膠修飾之NaYF4:Yb,Er@SiO2複合奈米粒子(NaYF4:Yb,Er@SiO2@GA)。透過利用穿透式電子顯微鏡(TEM)、高解析電子顯微鏡(HR-TEM)、X光繞射分析、傅立葉轉換紅外光(FIIR)光譜、界面電位(zeta potential)、及紫外光/可見光/近紅外光光譜(UV/VIS/NIR)分析產物之粒子形態、粒徑、晶體結構、界面電位及光學性質，可證實本研究成功製備出NaYF4:Yb,Er@SiO2@GA複合奈米粒子。將此NaYF4:Yb,Er@SiO2@GA複合奈米粒子作為藥物載體，發現它們並無顯著的生物毒性，但在負載抗癌藥物DOX後，可有效毒殺人類子宮頸癌細胞。此外，以近紅外雷射照射，它們因近紅外光上轉換特性亦可發出可見光螢光。如此，本研究所製備之NaYF4:Yb,Er@SiO2@GA複合奈米粒子兼具螢光生物顯影與藥物釋放的功能，預期在生醫應用上甚具潛力。

This thesis concerns the fabrication of NaYF4:Yb,Er/silica/gum arabic near-IR up-conversion composite nanoparticles and their application as a biomedical nanocarrier with the functions of bio-imaging and drug release simultaneously. For their fabrication, NaYF4:Yb,Er nanoparticles were obtained by ostwald ripening method at first. Then, SiO2 nanoshells were coated on NaYF4:Yb,Er nanoparticles by sol gel method in a reverse micellar system to yield the NaYF4:Yb,Er-SiO2 core-shell nanoparticles (NaYF4:Yb,Er@SiO2). After modification with (3-Aminopropyl)triethoxysilane to generate amino groups on the surface of SiO2 nanoshell, gum arabics (GA) were grafted onto the amino-functionalized surface of SiO2 via carbodiimide activation to form the GA-modified NaYF4:Yb,Er@SiO2 composite nanoparticles (NaYF4:Yb,Er@SiO2@GA). From the characterization of morphology, size, structure, functional group, zeta potential, and optical property by TEM, HR-TEM, XRD, RTIR, zeta potential, and UV/VIS/NIR spectrum, the successful fabrication of NaYF4:Yb,Er@SiO2@GA composite nanoparticles has been demonstrated. The NaYF4:Yb,Er@SiO2@GA composite nanoparticles were used as the drug carrier. It was found that they had no significant cytotoxicity but could kill the HeLa cells effectively after loading with the anti-cancer drug doxorubicin (DOX). Furthermore, they also could emit visible fluorescence while exposed to a near-IR laser owing to the near-IR up-conversion property. Thus, the resultant NaYF4:Yb,Er@SiO2@GA composite nanoparticles had the functions of fluorescence bio-imaging and drug release simultaneously and were expected to have great potential in the biomedical application.

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