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研究生: 歐雅靖
Setiyawan, Aussie Amalia
論文名稱: 鹽度及TMAH對工業廢水生物脫氮作用之影響評估
Effects of salinity and tetramethylammonium hydroxide (TMAH) on biological nitrogen removal for industrial wastewater
指導教授: 黃良銘
Whang, Liang-Ming,
學位類別: 碩士
Master
系所名稱: 工學院 - 環境工程學系
Department of Environmental Engineering
論文出版年: 2017
畢業學年度: 105
語文別: 英文
論文頁數: 73
外文關鍵詞: Salinity, organic solvent, ammonia, sequencing batch reactor (SBR), aerobic degradation
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  • The amount of produced industrial wastewater, especially the semiconductor and thin-film transistor liquid crystal display (TFT-LCD), increases due to an increasing production of these electronic items. The wastewater from those industrial sector contain many chemical such as salts and TMAH which is difficult to treat and suspected as inhibitor in nitrogen removal process. Nowadays, Biological nitrification and denitrification is the most studied process for nitrogen removal from wastewaters.
    This study evaluated the effects of salinity on the nitrification and denitrification of ammonia in two (aerobic and anoxic) sequencing batch reactors (SBRs). Aerobic SBR (O-SBR) and anoxic SBR (A-SBR) were tested with three salinity levels (1x, 2x, and 3x conductivity). For A-SBR, isopropyl acetate (IPA) was used as the bacterial carbon source. Nitrite accumulation was observed in the end nitrification process. The biomass level decreased with increasing salt concentration due to the adverse effect of salt on microorganisms. Increased salinity levels of microorganism resulted good nitrification and denitrification rate to treat high salinity containing wastewater.
    The nitrification performance of TMAH biodegradation using SBR under aerobic condition was also investigated in study. Results showed that complete nitrification achieved in the end of TMAH biodegradation. Batches were conducted to study the kinetics of nitrification in different TMAH concentrations. Specific ammonia oxidation rate had better result rather than specific nitrification rate with 0.97 mg-NH4/g-VSS hr treating 2500 mg/L TMAH.

    TABLE OF CONTENT ABSTRACT I ACKNOWLEDGMENT III TABLE OF CONTENT V LIST OF TABLE VII LIST OF FIGURES VIII Chapter 1 Introduction 1 Chapter 2 Literature Review 4 2.1 Sources and characteristics of TFT-LCD organic wastewater 4 2.2 Characteristics and degradation mechanisms of TMAH 5 2.3 Nitrogen Removal Process 7 2.3.1 Nitrogen Cycle 7 2.3.2 Effect of nitrogen to the aquatic system 9 2.3.3 Principal of Biological Removal of Nitrogen 11 2.3.4 Nitrification 12 2.3.5 Denitrification 14 2.4 Sequencing Batch Reactor 15 2.5 Nitrifying Bacteria in Wastewater Treatment 16 2.5.1 Ammonia Oxidizing Bacteria 18 2.5.2 Nitrite Oxidizing Bacteria 18 2.6 Molecular Methods Applied in Nitrifying Bacteria 20 2.6.1 Polymerase Chain Reaction (PCR) 21 2.6.2 Real-time PCR 24 Chapter 3 Materials and Methods 28 3.1 Configuration and operation of salinity sequencing batch reactors (SBRs) 28 3.2 Configuration and operation of TMAH biodegradation aerobic sequencing batch reactor (SBR) 30 3.3 Experimental Set-up 32 3.3.1 Effect salinity on nitrification for treating semiconductor wastewater 32 3.3.2 Effect salinity on denitrification for treating semiconductor wastewater 33 3.3.2 TMAH biodegradation SBR under aerobic condition 34 3.3.3 Batch test for nitrification performance in different TMAH levels under Aerobic Condition 34 3.4 Chemical Analytical methods 35 3.4.1 General analysis 35 3.4.2 Instrumental analysis 36 3.5 Molecular Biology Analytical Methods 36 3.5.1 DNA Extraction 36 3.5.2 DNA Concentration Measurement 37 3.5.3 Polymerase Chain Reaction (PCR) Amplification 37 3.5.4 Real-time PCR 41 Chapter 4 Results and Discussion 44 4.1 Performance of the salinity sequencing batch reactors (SBRs) 44 4.1.1 Nitrification performance of O-SBR 44 4.1.2 Denitrification performance of A-SBR 51 4.2 Performance of nitrification in TMAH biodegradation using SBR under aerobic condition 57 4.1.2 Performance of TMAH biodegradation SBR 57 Chapter 5 Conclusion and Suggestions 64 References 65

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